Transcript: Mouse NM_001199187.2

Mus musculus activating signal cointegrator 1 complex subunit 1 (Ascc1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Ascc1 (69090)
Length:
1492
CDS:
115..1185

Additional Resources:

NCBI RefSeq record:
NM_001199187.2
NBCI Gene record:
Ascc1 (69090)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001199187.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000245397 ACGTTCGATGGACGGAATTAT pLKO_005 142 CDS 100% 15.000 21.000 N Ascc1 n/a
2 TRCN0000183596 GAGGCTCAATTCTATTCACAT pLKO.1 1098 CDS 100% 4.950 6.930 N Ascc1 n/a
3 TRCN0000184617 GAGAACCATGACTGACTGGAT pLKO.1 1201 3UTR 100% 2.640 2.112 N Ascc1 n/a
4 TRCN0000241160 ATCACTGGTCAGCATCGAAAT pLKO_005 424 CDS 100% 10.800 7.560 N Ascc1 n/a
5 TRCN0000241158 CATGAAGAAGACGAGGATTTC pLKO_005 196 CDS 100% 10.800 7.560 N Ascc1 n/a
6 TRCN0000241159 CTTGGCTTCTGTCCTTCATTC pLKO_005 1333 3UTR 100% 10.800 7.560 N Ascc1 n/a
7 TRCN0000184635 CCTTGGCTTCTGTCCTTCATT pLKO.1 1332 3UTR 100% 5.625 3.938 N Ascc1 n/a
8 TRCN0000137029 CCTCAATGAAGTTGAGGTTCA pLKO.1 531 CDS 100% 0.405 0.284 N ASCC1 n/a
9 TRCN0000241157 GAGATCCTGCAGCGCTGTAAA pLKO_005 703 CDS 100% 13.200 7.920 N Ascc1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001199187.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03168 pDONR223 100% 83.6% 86.5% None (many diffs) n/a
2 ccsbBroad304_03168 pLX_304 0% 83.6% 86.5% V5 (many diffs) n/a
3 TRCN0000472778 GGTTATAGAGAGTGTTGGTATGTT pLX_317 46.1% 83.6% 86.5% V5 (many diffs) n/a
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