Construct: ORF TRCN0000472795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018812.2_s317c1
- Derived from:
- ccsbBroadEn_14785
- DNA Barcode:
- CAGCGTCCGAGACTGGTGCCCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKAR1B (5575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472795
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164758.1 | 99.5% | 33.1% | (many diffs) |
| 2 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164759.1 | 99.5% | 33.1% | (many diffs) |
| 3 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164760.2 | 99.5% | 33.1% | (many diffs) |
| 4 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164761.1 | 99.5% | 33.1% | (many diffs) |
| 5 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164762.1 | 99.5% | 33.1% | (many diffs) |
| 6 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_002735.2 | 99.5% | 33.1% | (many diffs) |
| 7 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | XM_011515446.2 | 85% | 18% | (many diffs) |
| 8 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | XM_017012433.1 | 61.9% | 4.2% | (many diffs) |
| 9 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | NM_001253890.1 | 85.5% | 28.7% | (many diffs) |
| 10 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | NM_008923.3 | 85.5% | 28.7% | (many diffs) |
| 11 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504660.3 | 85.5% | 28.7% | (many diffs) |
| 12 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504661.3 | 85.5% | 28.7% | (many diffs) |
| 13 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504663.2 | 85.5% | 28.7% | (many diffs) |
| 14 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_017320719.1 | 85.5% | 28.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 498
- ORF length:
- 429
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctccccg cccgcctgcc cctcggagga ggacgagagc ctgaagggct 121 gtgagctgta cgtgcagctg cacgggatcc agcaggtcct caaagactgt atcgtccacc 181 tctgcatctc caagcccgaa cgccccatga agttcctccg ggagcacttc gagaagctgg 241 agaaggaaga aaacaggcag attttggcgc ggcaaaagtc aaactcacag tcggactccc 301 atgatgagga ggtgtcgccc acccccccga accctgtggt gaaggcccgc cgccggcgag 361 gaggcgtgag tgccgaggtg tacaccgagg aggacgccgn gtcctacgtc aggaaaggtg 421 attccccaag gactacaaaa accatgactg cgctggccca aggccatctc caagaacgtg 481 ctcttcgctc acctggatga caacgagagg agtgacatat tcgatgccat gttccctgtc 541 actcacatcg ctggggagac tgttatacag caagggaatg aaggagacaa cttctatgtc 601 gttgatcaag gggaagtgga tgtgtacgtg aacggagagt gggtgaccaa catcagcgag 661 ggaggcagct tcggggagct ggcgctcatc tacggcaccc ccagggctgc gaccgtgaaa 721 gccaagacgg acctcaagct ctgggggatc gaccgggaca gctaccggcg catccttatg 781 ggcagcacgc tgaggaaacg caagatgtac gaggagttcc TCAGCAAGGT CTCCATCCTA 841 GAGTCCCTGG AGAAGTGGGA GCGTCTGACC GTGGCGGATG CGCTGGAGCC CGTCCAGTTT 901 GAAGATGGAG AGAAAATTGT GGTCCAGGGA GAGCCTGGGG ACGACTTTTA CATCATCACG 961 GAGGGCACCG CGTCCGTGCT GCAGCGCCGG TCCCCCAATG AGGAGTACGT GGAGGTGGGG 1021 CGCCTGGGAC CCTCTGACTA CTTCGGGGAG ATTGCACTGC TGCTGAACCG GCCCCGGGCG 1081 GCCACTGTCG TGGCCCGGGG GCCCCTCAAG TGTGTGAAGC TGGACCGGCC CCGCTTCGAG 1141 CGTGTGCTGG GGCCCTGCTC TGAGATCCTC AAGAGGAACA TTCAGCGTTA CAACAGCTTC 1201 ATCTCCCTCA CCGTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 1261 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT 1321 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGCGTCCG AGACTGGTGC 1381 CCCTTACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt