Construct: ORF TRCN0000472817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008101.1_s317c1
- Derived from:
- ccsbBroadEn_07046
- DNA Barcode:
- CCTAAATAGATCCTATTGTTCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEF (7008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472817
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7008 | TEF | TEF transcription factor, P... | NM_003216.4 | 98.2% | 98.3% | 462C>T;895_909del |
| 2 | human | 7008 | TEF | TEF transcription factor, P... | NM_001145398.2 | 84.6% | 83.6% | (many diffs) |
| 3 | mouse | 21685 | Tef | thyrotroph embryonic factor | NM_017376.3 | 89.6% | 95.7% | (many diffs) |
| 4 | mouse | 21685 | Tef | thyrotroph embryonic factor | NM_153484.3 | 80.8% | 83.6% | (many diffs) |
| 5 | mouse | 21685 | Tef | thyrotroph embryonic factor | XM_011245557.2 | 79.4% | 84.7% | (many diffs) |
| 6 | mouse | 21685 | Tef | thyrotroph embryonic factor | XM_011245558.2 | 71.7% | 74.1% | (many diffs) |
| 7 | mouse | 21685 | Tef | thyrotroph embryonic factor | XM_006520744.1 | 68.7% | 73.9% | (many diffs) |
| 8 | mouse | 21685 | Tef | thyrotroph embryonic factor | XM_011245559.1 | 60.9% | 65.4% | (many diffs) |
| 9 | mouse | 21685 | Tef | thyrotroph embryonic factor | XM_011245560.2 | 60.9% | 65.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgacgcgggc ggcggaaaga agccgcctgt ggacccgcag gcaggacccg 121 gtccggggcc ggggcgcgca gctggggaaa ggggcctgtc ggggtccttc cccctggtcc 181 tgaagaagct gatggagaac cccccgcgcg aggcgcgcct cgataaggaa aaggggaagg 241 aaaagctgga ggaggacgag gccgcagccg ccagcaccat ggctgtctca gcctccctca 301 tgccacccat ctgggacaag accatcccat atgatggcga atctttccac ctggagtaca 361 tggacctgga tgagttcctg ctggagaatg gcatccccgc cagccccacc cacctggccc 421 acaacctgct gctgcctgta gcagagctag aagggaagga gtctgccagc tcttccacag 481 catccccacc atcctcctcc actgccatct ttcagccctc tgaaactgtg tccagcacag 541 aatcttccct ggagaaggag agggagactc ccagtcccat cgaccccaat tgtgtggaag 601 tggatgtgaa cttcaatccg gaccccgccg accTGGTGCT CTCCAGTGTG CCAGGCGGGG 661 AGCTCTTCAA CCCTCGGAAG CACAAGTTTG CTGAGGAGGA CCTGAAGCCC CAGCCTATGA 721 TCAAAAAGGC CAAGAAGGTC TTTGTCCCCG ACGAGCAGAA GGATGAAAAG TACTGGACAA 781 GACGCAAGAA GAACAACGTG GCAGCTAAAC GGTCACGGGA TGCCCGGCGC CTGAAAGAGA 841 ATCAGATCAC CATCCGGGCA GCCTTCCTGG AGAAGGAGAA CACAGCCCTG CGGACGGAGG 901 TGGCCGAGCT ACGCAAGGAG GTGGGCAAGT GCAAGACCAT CGTGTCCAAG TATGAGACCA 961 AATACGGGCC CTTGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1021 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1081 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTAAATAGA TCCTATTGTT 1141 CCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt