Construct: ORF TRCN0000472832

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017546.1_s317c1
Derived from:
ccsbBroadEn_00440
DNA Barcode:
ACCTGGGATCTTATTCATTATCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GSDME (1687)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472832
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1687 GSDME gasdermin E NM_001127453.2 100% 100%
2 human 1687 GSDME gasdermin E NM_004403.3 100% 100%
3 human 1687 GSDME gasdermin E XM_024446670.1 100% 100%
4 human 1687 GSDME gasdermin E NM_001127454.2 66.9% 66.9% 0_1ins492
5 human 1687 GSDME gasdermin E XM_017011802.1 66.9% 66.9% 0_1ins492
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1557
ORF length:
1488
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtttgccaaa gcaaccagga attttcttag agaagttgat gctgatggtg
121acctgattgc agtatcaaat ctgaatgact ctgataagtt acagcttcta agtctggtga
181caaaaaagaa gagattctgg tgctggcaga gacccaagta ccagttttta tccctcaccc
241ttggcgatgt actcatagaa gaccaatttc cgagtccagt ggtcgtggag tcggactttg
301tgaaatacga gggcaagttt gcaaaccacg tgagtggaac cctggagact gcactgggga
361aggtcaagct gaacctgggg ggcagcagcc gcgtagagag ccagtcttca tttggaaccc
421tgaggaagca ggaggtggat ttgcagcagc tcatcagaga ctctgccgag agaacaataa
481atctgagaaa ccctgtgctc cagcaggtgc tggaaggaag gaatgaggtc ctgtgcgttt
541tgacacagaa gatcacgacg atgcagaagt gtgtgatctc tgagcacatg caggtcgagg
601agaagtgtgg tggcatcgtg ggcatccaga ccaagacggt gcaggtgtca gcgacggagg
661atgggaatgt caccaaggac tccaacgtgg tgctggagat cccagctgcc accaccattg
721cctacggtgt cattgagtta tacgtgaaac tggacggcca gttcgagttc tgccttctcc
781gagggaagca aggtggcttc gagaacaaga agagaattga ctctgtctac ctggaccccc
841tggtctttcg agagtttgca ttcatagaca tgccagatgc tgcgcatggg atatcttccc
901aggatggacc attaagtgtt ttaaagcaag cgaccctgct cctggagagg aatttccatc
961catttgcgga gctgcctgag ccacaacaga cagctttgag tgacatcttc caggcggtcc
1021tatttgatga tgaactactc atggtcctgg aaccagtgtg cgatgacctg gtcagcggcc
1081tctcgcccac agtGGCGGTG CTGGGGGAGC TGAAGCCCCG GCAGCAGCAG GACCTTGTGG
1141CCTTCCTGCA GCTGGTGGGG TGCAGCTTAC AGGGTGGGTG TCCGGGCCCC GAGGATGCAG
1201GCAGCAAGCA GCTGTTTATG ACAGCCTACT TCTTGGTCAG TGCCCTCGCA GAAATGCCAG
1261ATAGCGCAGC AGCTCTGCTG GGCACTTGCT GCAAACTCCA GATCATTCCC ACACTGTGCC
1321ACTTGCTTCG TGCTCTGTCT GATGATGGAG TATCTGATCT TGAAGACCCA ACCTTGACTC
1381CCCTGAAAGA TACAGAAAGG TTTGGGATTG TGCAGCGCTT GTTTGCCTCA GCTGACATTA
1441GTCTGGAGAG ACTGAAGTCA TCTGTGAAAG CTGTCATTCT GAAGGACTCT AAAGTCTTCC
1501CACTGCTTCT TTGTATAACC CTGAATGGAC TCTGTGCTTT AGGCAGAGAA CATTCATTGC
1561CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1621TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1681ATATATCTTG TGGAAAGGAC GAACCTGGGA TCTTATTCAT TATCTAACGC GTTAAGTCga
1741caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)