Construct: ORF TRCN0000472875

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016915.1_s317c1
Derived from:
ccsbBroadEn_06857
DNA Barcode:
CTCCCTATCTCTTAGGGTCGCCCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RFC4 (5984)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472875
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5984 RFC4 replication factor C subunit 4 NM_002916.4 99.9% 100% 798C>T
2 human 5984 RFC4 replication factor C subunit 4 NM_181573.2 99.9% 100% 798C>T
3 mouse 106344 Rfc4 replication factor C (activ... NM_145480.1 86.9% 90.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1155
ORF length:
1089
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca agcatttctt aaaggtacat ccatcagtac taaacccccg ctgaccaagg
121atcgaggagt agctgccagt gcgggaagta gcggagagaa caagaaagcc aaacccgttc
181cctgggtgga aaaatatcgc ccaaaatgtg tggatgaagt tgctttccag gaagaagtgg
241ttgcagtgct gaaaaaatct ttagaaggag cagatcttcc taatctcttg ttttacggac
301cacctggaac tggaaaaaca tccactattt tggcagcagc tagagaactc tttgggcctg
361aacttttccg attaagagtt cttgagttaa atgcatctga tgaacgtgga atacaagtag
421ttcgagagaa agtgaaaaat tttgctcaat taactgtgtc aggaagtcgc tcagatggga
481agccgtgtcc gccttttaag attgtgattc tggatgaagc agattctatg acctcagctg
541ctcaggcagc tttaagacgt accatggaga aggagtcgaa aaccacccga ttctgtctta
601tctGTAACTA TGTCAGTCGA ATAATTGAAC CCCTGACCTC TAGATGTTCA AAATTCCGCT
661TCAAGCCTCT GTCAGATAAA ATTCAACAGC AGCGATTACT AGACATTGCC AAGAAGGAAA
721ATGTCAAAAT TAGTGATGAG GGAATAGCTT ATCTTGTTAA AGTGTCAGAA GGAGACTTAA
781GAAAAGCCAT TACATTTCTT CAAAGCGCTA CTCGATTAAC AGGTGGAAAG GAGATCACAG
841AGAAAGTGAT TACAGACATT GCTGGGGTAA TACCAGCTGA GAAAATTGAT GGAGTATTTG
901CTGCCTGTCA GAGTGGCTCT TTTGACAAAC TAGAAGCTGT GGTCAAGGAT TTAATAGATG
961AGGGTCATGC AGCAACTCAG CTCGTCAATC AACTCCATGA TGTGGTTGTA GAAAATAACT
1021TATCTGATAA ACAGAAGTCT ATTATCACAG AAAAACTTGC CGAAGTTGAC AAATGCCTAG
1081CAGATGGTGC TGATGAACAT TTGCAACTCA TCAGCCTTTG TGCAACTGTG ATGCAGCAGT
1141TATCTCAGAA TTGTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1201CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1261TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTCCCTATCT CTTAGGGTCG
1321CCCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)