Construct: ORF TRCN0000472880

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007027.1_s317c1
Derived from:
ccsbBroadEn_01932
DNA Barcode:
GCCGCAAAGCACGGCGATCTAAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPST1 (8460)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472880
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8460 TPST1 tyrosylprotein sulfotransfe... NM_003596.4 100% 100%
2 human 8460 TPST1 tyrosylprotein sulfotransfe... XM_017012726.2 100% 100%
3 human 8460 TPST1 tyrosylprotein sulfotransfe... XM_017012727.2 100% 100%
4 human 8460 TPST1 tyrosylprotein sulfotransfe... XM_005250642.3 96.7% 97% (many diffs)
5 human 8460 TPST1 tyrosylprotein sulfotransfe... XM_017012724.1 96.7% 97% (many diffs)
6 human 8460 TPST1 tyrosylprotein sulfotransfe... XM_011516634.3 95.4% 95.4% 1043_1044ins51
7 human 8460 TPST1 tyrosylprotein sulfotransfe... XM_011516635.3 95.4% 95.4% 1043_1044ins51
8 mouse 22021 Tpst1 protein-tyrosine sulfotrans... NM_001130476.2 89.3% 96.4% (many diffs)
9 mouse 22021 Tpst1 protein-tyrosine sulfotrans... NM_013837.2 89.3% 96.4% (many diffs)
10 mouse 22021 Tpst1 protein-tyrosine sulfotrans... XM_006504418.2 85.3% 91.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1176
ORF length:
1110
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt tggaaagctg aagcagaact tactattggc atgtctggtg attagttctg
121tgactgtgtt ttacctgggc cagcatgcca tggaatgcca tcaccggata gaggaacgta
181gccagccagt caaattggag agcacaagga ccactgtgag aactggcctg gacctcaaag
241ccaacaaaac ctttgcctat cacaaagata tgcctttaat atttattgga ggtgtgcctc
301ggagtggaac cacactcatg agggccatgc tggacgcaca tcctgacatt cgctgtggag
361aggaaaccag ggtcattccc cgaatcctgg ccctgaagca gatgtggtca cggtcaagta
421aagagaagat ccgcctggat gaggctggtg ttactgatga agtgctggat tctgccatgc
481aagccttctt actagaaatt atcgttaagc atggggagcc agccccttat ttatgtaata
541aagatccttt tgccctgaaa tctttaactt acctttctag gttattcccc aatgccaaat
601ttctcctgat ggtccgagat ggCCGGGCAT CAGTACATTC AATGATTTCT CGAAAAGTTA
661CTATAGCTGG ATTTGATCTG AACAGCTATA GGGACTGTTT GACAAAGTGG AATCGTGCTA
721TAGAGACCAT GTATAACCAG TGTATGGAGG TTGGTTATAA AAAGTGCATG TTGGTTCACT
781ATGAACAACT TGTCTTACAT CCTGAACGGT GGATGAGAAC ACTCTTAAAG TTCCTCCAGA
841TTCCATGGAA CCACTCAGTA TTGCACCATG AAGAGATGAT TGGGAAAGCT GGGGGAGTGT
901CTCTGTCAAA AGTGGAGAGA TCTACAGACC AAGTAATCAA GCCAGTCAAT GTAGGAGCTC
961TATCAAAATG GGTTGGGAAG ATACCGCCAG ATGTTTTACA AGACATGGCA GTGATTGCTC
1021CTATGCTTGC CAAGCTTGGA TATGACCCAT ATGCCAACCC ACCTAACTAC GGAAAACCTG
1081ATCCCAAAAT TATTGAAAAC ACTCGAAGGG TCTATAAGGG AGAATTCCAA CTACCTGACT
1141TTCTTAAAGA AAAACCACAG ACTGAGCAAG TGGAGTACCC AACTTTCTTG TACAAAGTGG
1201TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1261CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1321AGCCGCAAAG CACGGCGATC TAAACACGCG TTAAGTCgac aatcaacctc tggattacaa
1381aatttgtgaa agatt

Download FASTA (ORF) (Full)