Construct: ORF TRCN0000472880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007027.1_s317c1
- Derived from:
- ccsbBroadEn_01932
- DNA Barcode:
- GCCGCAAAGCACGGCGATCTAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPST1 (8460)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472880
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | NM_003596.4 | 100% | 100% | |
| 2 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | XM_017012726.2 | 100% | 100% | |
| 3 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | XM_017012727.2 | 100% | 100% | |
| 4 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | XM_005250642.3 | 96.7% | 97% | (many diffs) |
| 5 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | XM_017012724.1 | 96.7% | 97% | (many diffs) |
| 6 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | XM_011516634.3 | 95.4% | 95.4% | 1043_1044ins51 |
| 7 | human | 8460 | TPST1 | tyrosylprotein sulfotransfe... | XM_011516635.3 | 95.4% | 95.4% | 1043_1044ins51 |
| 8 | mouse | 22021 | Tpst1 | protein-tyrosine sulfotrans... | NM_001130476.2 | 89.3% | 96.4% | (many diffs) |
| 9 | mouse | 22021 | Tpst1 | protein-tyrosine sulfotrans... | NM_013837.2 | 89.3% | 96.4% | (many diffs) |
| 10 | mouse | 22021 | Tpst1 | protein-tyrosine sulfotrans... | XM_006504418.2 | 85.3% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tggaaagctg aagcagaact tactattggc atgtctggtg attagttctg 121 tgactgtgtt ttacctgggc cagcatgcca tggaatgcca tcaccggata gaggaacgta 181 gccagccagt caaattggag agcacaagga ccactgtgag aactggcctg gacctcaaag 241 ccaacaaaac ctttgcctat cacaaagata tgcctttaat atttattgga ggtgtgcctc 301 ggagtggaac cacactcatg agggccatgc tggacgcaca tcctgacatt cgctgtggag 361 aggaaaccag ggtcattccc cgaatcctgg ccctgaagca gatgtggtca cggtcaagta 421 aagagaagat ccgcctggat gaggctggtg ttactgatga agtgctggat tctgccatgc 481 aagccttctt actagaaatt atcgttaagc atggggagcc agccccttat ttatgtaata 541 aagatccttt tgccctgaaa tctttaactt acctttctag gttattcccc aatgccaaat 601 ttctcctgat ggtccgagat ggCCGGGCAT CAGTACATTC AATGATTTCT CGAAAAGTTA 661 CTATAGCTGG ATTTGATCTG AACAGCTATA GGGACTGTTT GACAAAGTGG AATCGTGCTA 721 TAGAGACCAT GTATAACCAG TGTATGGAGG TTGGTTATAA AAAGTGCATG TTGGTTCACT 781 ATGAACAACT TGTCTTACAT CCTGAACGGT GGATGAGAAC ACTCTTAAAG TTCCTCCAGA 841 TTCCATGGAA CCACTCAGTA TTGCACCATG AAGAGATGAT TGGGAAAGCT GGGGGAGTGT 901 CTCTGTCAAA AGTGGAGAGA TCTACAGACC AAGTAATCAA GCCAGTCAAT GTAGGAGCTC 961 TATCAAAATG GGTTGGGAAG ATACCGCCAG ATGTTTTACA AGACATGGCA GTGATTGCTC 1021 CTATGCTTGC CAAGCTTGGA TATGACCCAT ATGCCAACCC ACCTAACTAC GGAAAACCTG 1081 ATCCCAAAAT TATTGAAAAC ACTCGAAGGG TCTATAAGGG AGAATTCCAA CTACCTGACT 1141 TTCTTAAAGA AAAACCACAG ACTGAGCAAG TGGAGTACCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AGCCGCAAAG CACGGCGATC TAAACACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt