Construct: ORF TRCN0000472902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016496.1_s317c1
- Derived from:
- ccsbBroadEn_00166
- DNA Barcode:
- ATCCAAGTAAGTCGTATTTTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMP5 (653)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653 | BMP5 | bone morphogenetic protein 5 | NM_021073.4 | 100% | 100% | |
| 2 | human | 653 | BMP5 | bone morphogenetic protein 5 | NM_001329754.2 | 91.8% | 91.8% | 1103_1104ins111 |
| 3 | human | 653 | BMP5 | bone morphogenetic protein 5 | XM_011514817.3 | 77.9% | 74.4% | (many diffs) |
| 4 | human | 653 | BMP5 | bone morphogenetic protein 5 | NM_001329756.2 | 76.4% | 75% | (many diffs) |
| 5 | human | 653 | BMP5 | bone morphogenetic protein 5 | XM_024446524.1 | 50.4% | 50.2% | 682_683insG;687_688ins674 |
| 6 | mouse | 12160 | Bmp5 | bone morphogenetic protein 5 | NM_007555.4 | 89.4% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1428
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tctgactgta tttttactta agggtattgt gggtttcctc tggagctgct 121 gggttctagt gggttatgca aaaggaggtt tgggagacaa tcatgttcac tccagtttta 181 tttatagaag actacggaac cacgaaagac gggaaataca aagggaaatt ctctctatct 241 tgggtttgcc tcacagaccc agaccatttt cacctggaaa acaagcgtcc tctgcacctc 301 tctttatgct ggatctctac aatgccatga ccaatgaaga aaatcctgaa gagtcggagt 361 actcagtaag ggcatccttg gcagaagaga ccagaggggc aagaaaggga tacccagcct 421 ctcccaatgg gtatcctcgt cgcatacagt tatctcggac gactcctctg accacccaga 481 gtcctcctct agccagcctc catgatacca actttctgaa tgatgctgac atggtcatga 541 gctttgtcaa cttagttgaa agagacaagg atttttctca ccagcgaagg cattacaaag 601 aatttcgatt tgatcttacc caaattcctc atggagaggc agtgacagca gctgaattcc 661 ggatatacaa ggaccggagc aacaaccgat ttgaaaatga aacaattaag attagcatat 721 atcaaatcat caaggaatac acaaataggg atgcagatct gttcttgtta gacacaagaa 781 aggcccaagc tttagatgtg ggttggcttg tctttgatat cactgtgacc agcaatcatt 841 gggtgattaa tccccagaat aatttgggct tacagctctg tgcagaaaca ggggatggac 901 gcagtatcaa cgtaaaatct gctggtcttg tgggaagaca gggacctcag tcaaaacaac 961 cattcatggt ggccTTCTTC AAGGCGAGTG AGGTACTTCT TCGATCCGTG AGAGCAGCCA 1021 ACAAACGAAA AAATCAAAAC CGCAATAAAT CCAGCTCTCA TCAGGACTCC TCCAGAATGT 1081 CCAGTGTTGG AGATTATAAC ACAAGTGAGC AAAAACAAGC CTGTAAGAAG CACGAACTCT 1141 ATGTGAGCTT CCGGGATCTG GGATGGCAGG ACTGGATTAT AGCACCAGAA GGATACGCTG 1201 CATTTTATTG TGATGGAGAA TGTTCTTTTC CACTTAACGC CCATATGAAT GCCACCAACC 1261 ACGCTATAGT TCAGACTCTG GTTCATCTGA TGTTTCCTGA CCACGTACCA AAGCCTTGTT 1321 GTGCTCCAAC CAAATTAAAT GCCATCTCTG TTCTGTACTT TGATGACAGC TCCAATGTCA 1381 TTTTGAAAAA ATATAGAAAT ATGGTAGTAC GCTCATGTGG CTGCCACAAC CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGAATCCAAG TAAGTCGTAT TTTTTTAACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt