Construct: ORF TRCN0000472977
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007925.1_s317c1
- Derived from:
- ccsbBroadEn_09049
- DNA Barcode:
- GAGCAGCCAAATAGGCTGCGAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B9D2 (80776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472977
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80776 | B9D2 | B9 domain containing 2 | NM_030578.4 | 99.8% | 99.4% | 33A>G |
| 2 | human | 80776 | B9D2 | B9 domain containing 2 | XM_011527349.2 | 99.8% | 99.4% | 33A>G |
| 3 | human | 80776 | B9D2 | B9 domain containing 2 | XM_011527350.2 | 69.7% | 69.7% | 0_1ins159 |
| 4 | mouse | 232987 | B9d2 | B9 protein domain 2 | NM_172148.1 | 88% | 96% | (many diffs) |
| 5 | mouse | 232987 | B9d2 | B9 protein domain 2 | XM_006539854.3 | 88% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaggtgcac gtgatcgggc agatcatggg ggccagcggt ttctcggaaa 121 gtagcctctt ctgcaagtgg ggcattcaca caggggcggc atggaagctc ctgtcaggcg 181 tgcgggaggg ccaaacgcaa gtggacaccc cgcagatagg ggacatggct tactgGTCCC 241 ACCCCATCGA CCTGCACTTC GCCACCAAAG GTCTTCAAGG CTGGCCCCGG CTCCATTTCC 301 AGGTGTGGTC CCAGGACAGC TTTGGCCGCT GCCAGCTTGC AGGCTATGGA TTTTGCCATG 361 TGCCCAGTAG CCCGGGCACC CACCAGCTGG CCTGCCCCAC GTGGCGGCCC CTGGGCAGTT 421 GGCGAGAACA GTTGGCACGG GCTTTCGTGG GTGGTGGGCC GCAGCTGCTG CATGGGGACA 481 CCATCTACAG TGGGGCCGAC CGCTATCGCC TGCACACAGC TGCTGGTGGC ACCGTGCACC 541 TGGAGATCGG CCTGCTGCTC CGCAACTTCG ACCGCTACGG CGTGGAGTGC TGCCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGAGAGC AGCCAAATAG GCTGCGAAAT ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt