Construct: ORF TRCN0000472987
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014777.1_s317c1
- Derived from:
- ccsbBroadEn_13694
- DNA Barcode:
- ATCTCCGTTAAATGTTGACGAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTHFD2L (441024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472987
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008230.1 | 61.5% | 57.9% | (many diffs) |
| 2 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008234.1 | 61.3% | 57.2% | (many diffs) |
| 3 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001351310.1 | 61.3% | 57.8% | (many diffs) |
| 4 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001351311.1 | 61.3% | 57.8% | (many diffs) |
| 5 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008229.1 | 61.3% | 57.8% | (many diffs) |
| 6 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001351314.2 | 59.4% | 55.4% | (many diffs) |
| 7 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008228.1 | 58% | 54.2% | (many diffs) |
| 8 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001004346.4 | 47.7% | 46.3% | (many diffs) |
| 9 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_005265690.2 | 46.8% | 45.4% | (many diffs) |
| 10 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008225.1 | 46.8% | 45.4% | (many diffs) |
| 11 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008226.1 | 46.8% | 45.4% | (many diffs) |
| 12 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008224.1 | 42.2% | 40.9% | (many diffs) |
| 13 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001144978.2 | 39.8% | 38.6% | (many diffs) |
| 14 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008221.2 | 36.1% | 34.2% | (many diffs) |
| 15 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008220.2 | 36% | 34.3% | (many diffs) |
| 16 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008222.2 | 36% | 33.9% | (many diffs) |
| 17 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008223.2 | 35.5% | 34.6% | (many diffs) |
| 18 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008219.2 | 35.1% | 32.8% | (many diffs) |
| 19 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008218.2 | 30.4% | 29.3% | (many diffs) |
| 20 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741223.2 | 25.8% | 1_805del;1111_1280del;1340_1341ins73 | |
| 21 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NR_147097.2 | 16.6% | (many diffs) | |
| 22 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741224.2 | 14.7% | (many diffs) | |
| 23 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741222.2 | 14.6% | (many diffs) | |
| 24 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | XM_006535177.3 | 38.1% | 40.1% | (many diffs) |
| 25 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | NM_026788.1 | 36.4% | 38.4% | (many diffs) |
| 26 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | XM_006535176.3 | 33.6% | 35.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cccaagagtc agcggtatat tagttcagtt accactacca gaccacgttG 121 ATGAGCGAAC AATATGCAAT GGAATTGCCC CAGAAAAAGA TGTAGATGGA TTTCATATTA 181 TCAATATTGG AAGATTGTGC CTTGATCAGC ATTCTCTCAT ACCTGCCACT GCCAGTGCTG 241 TTTGGGAAAT AATAAAAAGA ACAGGAATTC AAACATTTGG AAAAAATGTG GTTGTGGCTG 301 GAAGATCCAA GAACGTAGGG ATGCCTATTG CCATGCTTTT ACACACTGAT GGAGAGCATG 361 AACGGCCAGG AGGTGATGCA ACTGTGACAA TAGCTCACAG ATACACCCCC AAAGAGCAAC 421 TGAAGATTCA TACGCAGCTG GCAGATATTA TCATAGTTGC TGCAGAGAGA TTTCACCATT 481 TTGCCCAGGA CCTCTCCAAC TCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCTCCGTTAA 661 ATGTTGACGA ACGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att