Construct: ORF TRCN0000473034
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009980.1_s317c1
- Derived from:
- ccsbBroadEn_03598
- DNA Barcode:
- CTCTTACCGCGGATAGGTAGGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35E3 (55508)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473034
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55508 | SLC35E3 | solute carrier family 35 me... | NM_018656.5 | 100% | 100% | |
| 2 | human | 55508 | SLC35E3 | solute carrier family 35 me... | NM_001354997.3 | 88.1% | 88.1% | 400_401ins111 |
| 3 | human | 55508 | SLC35E3 | solute carrier family 35 me... | NM_001354998.2 | 84% | 81.4% | (many diffs) |
| 4 | human | 55508 | SLC35E3 | solute carrier family 35 me... | NR_149144.3 | 5% | (many diffs) | |
| 5 | human | 55508 | SLC35E3 | solute carrier family 35 me... | NR_149143.3 | 5% | (many diffs) | |
| 6 | mouse | 215436 | Slc35e3 | solute carrier family 35, m... | NM_029875.2 | 89.4% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1005
- ORF length:
- 939
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc attgctggtg gaccgagtgc ggggccactg gcgaatcgcc gccgggctcc 121 tgttcaacct gctggtgtcc atctgcattg tgttcctcaa caaatggatt tatgtgtacc 181 acggcttccc caacatgagc ctgaccctgg tgcacttcgt ggtcacctgg ctgggcttgt 241 atatctgcca gaagctggac atctttgccc ccaaaagtct gccgccctcc aggctcctcc 301 tcctggccct cagcttctgt ggctttgtgg tcttcactaa cctttctctg cagaacaaca 361 ccataggcac ctatcagctg gccaaggcca tgaccacgcc ggtgatcata gccatccaga 421 ccttctgcta ccagaaaacc ttctccacca gaatccagct cacgctgatt cctataactt 481 taggtgtaat ccTAAATTCT TATTACGATG TGAAGTTTAA TTTCCTTGGA ATGGTGTTTG 541 CTGCTCTTGG TGTTTTAGTT ACATCCCTTT ATCAAGTGTG GGTAGGAGCC AAACAGCATG 601 AATTACAAGT GAACTCAATG CAGCTGCTGT ACTACCAGGC TCCGATGTCA TCTGCCATGT 661 TGCTGGTTGC TGTGCCCTTC TTTGAGCCAG TGTTTGGAGA AGGAGGAATA TTTGGTCCCT 721 GGTCAGTTTC TGCTTTGCTT ATGGTGCTGC TATCTGGAGT AATAGCTTTC ATGGTGAACT 781 TATCAATTTA TTGGATCATT GGGAACACTT CACCTGTCAC CTATAACATG TTCGGACACT 841 TCAAGTTCTG CATTACTTTA TTCGGAGGAT ATGTTTTATT TAAGGATCCA CTGTCCATTA 901 ATCAGGCCCT TGGCATTTTA TGTACATTAT TTGGCATTCT CGCCTATACC CACTTTAAGC 961 TCAGTGAACA GGAAGGAAGT AGGAGTAAAC TGGCACAACG TCCTTACCCA ACTTTCTTGT 1021 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1081 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT TATCTTGTGG 1141 AAAGGACGAC TCTTACCGCG GATAGGTAGG CTTACGCGTT AAGTCgacaa tcaacctctg 1201 gattacaaaa tttgtgaaag att