Construct: ORF TRCN0000473044
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017290.1_s317c1
- Derived from:
- ccsbBroadEn_13046
- DNA Barcode:
- ACGGGCCTAAATAGGACCCCTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NT5C3B (115024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473044
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | XM_006721669.3 | 99.4% | 99.2% | (many diffs) |
| 2 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | XM_006721670.3 | 99.4% | 99.2% | (many diffs) |
| 3 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | NM_052935.5 | 85.2% | 85% | (many diffs) |
| 4 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | XM_011524277.3 | 64.9% | 65% | 1_105del;303A>G;543_544ins201 |
| 5 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | XM_011524276.2 | 63.2% | 63.3% | 1_129del;327A>G;567_568ins201 |
| 6 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | XM_017024127.1 | 51.2% | 33.3% | (many diffs) |
| 7 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | NR_033464.1 | 51.2% | (many diffs) | |
| 8 | human | 115024 | NT5C3B | 5'-nucleotidase, cytosolic ... | NR_033465.1 | 46.3% | (many diffs) | |
| 9 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_017314726.1 | 83.5% | 85.3% | (many diffs) |
| 10 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_017314724.1 | 81.1% | 82.9% | (many diffs) |
| 11 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_011249201.1 | 78.6% | 77.4% | (many diffs) |
| 12 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_017314727.1 | 78.6% | 77.4% | (many diffs) |
| 13 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_017314728.1 | 78.6% | 77.4% | (many diffs) |
| 14 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | NM_001102650.1 | 74.3% | 76% | (many diffs) |
| 15 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_017314723.1 | 72.4% | 74% | (many diffs) |
| 16 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_006534046.3 | 66.9% | 68.4% | (many diffs) |
| 17 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_006534047.3 | 66.9% | 68.4% | (many diffs) |
| 18 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | NM_026561.4 | 62.4% | 64% | (many diffs) |
| 19 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_006534043.3 | 62.4% | 64% | (many diffs) |
| 20 | mouse | 68106 | Nt5c3b | 5'-nucleotidase, cytosolic ... | XM_017314725.1 | 60.8% | 62.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tcggcatgac cttgagcagg tttgcatata atggaaagcg atgcccttct tcttacaata 121 ttctggataa tagcaagatc atcagtgagg agtgtcggaa agagctcaca gcgctccttc 181 accactatta cccaattgag atcgacccac accggaccgt caaggagaag ctacctcata 241 tggtggaatg gtggaccaaa gcgcacaatc tcctatgtca gcagaagatt cagaagtttc 301 agatagccca ggtggttaga gagtccaatg caatgctcag ggagggatat aagaccTTCT 361 TCAACACACT CTACCATAAC AACATTCCCC TTTTCATCTT TTCTGCGGGC ATTGGTGATA 421 TCCTGGAAGA AATTATCCGA CAGATGAAAG TGTTCCACCC CAACATCCAC ATCGTGTCTA 481 ACTACATGGA TTTTAATGAA GATGGTTTTC TCCAGGGATT TAAGGGCCAG CTGATACACA 541 CATACAACAA GAACAGCTCT GTGTGTGAGA ACTGTGGTTA CTTCCAGCAA CTTGAGGGCA 601 AAACCAATGT CATCCTGCTG GGAGACTCTA TCGGGGACCT CACCATGGCC GATGGGGTTC 661 CTGGTGTGCA GAACATTCTC AAAATTGGCT TCCTGAATGA CAAGGTGGAG GAGCGGCGGG 721 AGCGCTACAT GGACTCCTAT GACATCGTGC TGGAGAAGGA CGAGACTCTG GATGTGGTCA 781 ACGGGCTACT GCAGCACATC CTGTGCCAGG GGGTCCAGCT GGAGATGCAA GGCCCCTGCC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GAACGGGCCT AAATAGGACC CCTGATACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt