Construct: ORF TRCN0000473045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013745.1_s317c1
- Derived from:
- ccsbBroadEn_09257
- DNA Barcode:
- AAAAAAGTTGCCCGATGAACTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM5 (85363)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473045
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85363 | TRIM5 | tripartite motif containing 5 | NM_033092.3 | 99.9% | 100% | 477A>G |
| 2 | human | 85363 | TRIM5 | tripartite motif containing 5 | NM_033093.3 | 89.4% | 83.1% | (many diffs) |
| 3 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_005253184.3 | 89.4% | 83.1% | (many diffs) |
| 4 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_017018462.2 | 86.2% | 85.8% | (many diffs) |
| 5 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_017018460.2 | 80.4% | 69.4% | (many diffs) |
| 6 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_011520426.3 | 78.1% | 74.2% | (many diffs) |
| 7 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_017018461.2 | 78.1% | 74.2% | (many diffs) |
| 8 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_011520427.2 | 78% | 72.3% | 477A>G;742_743ins23;813_814ins205 |
| 9 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_006718358.3 | 76.1% | 74.9% | 477A>G;765_766ins101;795_795delTins146 |
| 10 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_017018463.2 | 76.1% | 74.9% | 477A>G;765_766ins101;795_795delTins146 |
| 11 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_930921.2 | 66.6% | (many diffs) | |
| 12 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_001748017.2 | 66.5% | (many diffs) | |
| 13 | human | 85363 | TRIM5 | tripartite motif containing 5 | NM_033034.3 | 65% | 60% | (many diffs) |
| 14 | human | 85363 | TRIM5 | tripartite motif containing 5 | XM_005253183.3 | 65% | 60% | (many diffs) |
| 15 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_001748014.2 | 64.5% | (many diffs) | |
| 16 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_001748018.2 | 61.9% | (many diffs) | |
| 17 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_001748015.2 | 61.4% | (many diffs) | |
| 18 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_001748016.2 | 29.5% | (many diffs) | |
| 19 | human | 85363 | TRIM5 | tripartite motif containing 5 | XR_930920.3 | 28.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1107
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttctggaatc ctggttaatg taaaggagga ggtgacctgc cccatctgcc 121 tggaactcct gacacaaccc ctgagcctgg actgcggcca cagcttctgc caagcatgcc 181 tcactgcaaa ccacaagaag tccatgctag acaaaggaga gagtagctgc cctgtgtgcc 241 ggatcagtta ccagcctgag aacatacggc ctaatcggca tgtagccaac atagtggaga 301 agctcaggga ggtcaagttg agcccagagg ggcagaaagt tgatcattgt gcacgccatg 361 gagagaaact tctactcttc tgtcaggagg acgggaaggt catttgctgg ctttgtgagc 421 ggtctcagga gcaccgtggt caccacacgt tcctcacaga ggaggttgcc cgggagtacc 481 aagtgaagct ccaggcagct ctggagatgc tgaggcagaa gcagcaggaa gctgaagagt 541 tggaagctga catcagagaa gagaaagctt cctggaagac tcaaatacag tatgacaaaa 601 ccaacgtctt ggcagatttt gagcaactga gagacatcct ggactgggag gagagcaatg 661 agctgcaaaa cctggagaag gaggaggaag acattctgaa aagccttacg aactctgaaa 721 ctgagatggt gcagcagacc cagtccctga gagagctcat ctcagatctg gagcatcggc 781 tgcaggggtc agtgatggag ctgcttcagg gtgtggatGG CGTCATAAAA AGGACGGAGA 841 ACGTGACCTT GAAGAAGCCA GAAACTTTTC CAAAAAATCA AAGGAGAGTG TTTCGAGCTC 901 CTGATCTGAA AGGAATGCTA GAAGTGTTTA GAGAGCTGAC AGATGTCCGA CGCTACTGGG 961 GTAAGGAGAA GTCACATTAT CATAAGCCAC CCTGCGGCTT ATCATTATTA TTATCTTTAT 1021 CTTTTAGAAT TTTATGTTCT CTATTAGGCT CATGTTTTAA GATTTATGAT TCTCCTTCCA 1081 AGACACACAT AACTTACCCC TCCTTATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1141 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1201 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAAAAAGT 1261 TGCCCGATGA ACTGAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1321 aagatt