Construct: ORF TRCN0000473047

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004812.1_s317c1
Derived from:
ccsbBroadEn_13218
DNA Barcode:
ATGGACTATCATGTATGCTTCAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SESN3 (143686)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473047
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 143686 SESN3 sestrin 3 NM_144665.4 64.1% 62.7% (many diffs)
2 human 143686 SESN3 sestrin 3 XM_011542614.1 59.2% 57.7% (many diffs)
3 human 143686 SESN3 sestrin 3 XM_024448355.1 48.3% 47% (many diffs)
4 human 143686 SESN3 sestrin 3 NM_001271594.2 36% 34.8% (many diffs)
5 mouse 75747 Sesn3 sestrin 3 NM_030261.4 57.6% 57.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1029
ORF length:
963
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ccggggcggc ggcagcccgt cggccgccgc caactacctg ctctgtacca
121actgccggaa agtgctgcgg aaggataaaa gaatcagagt gtctcaaccc ttgacaagag
181gaccaagtgc ctttattcca gagaaggaag ttgtccaagc aaacacagtg gatgaacgta
241ctaactttct tgtggaagaa tactctacat ccggtcgtct ggacaacatc acacaggtca
301tgagtttaca cactcagtac ctggagtctt tcttgcggag ccagttttac atgttgcgca
361tggatggtcc ccttcctcta ccatacaggc actatattgc aataatggct gcagctagac
421atcagtgttc ttacttaata aacatgcatg tggatgaatt tttaaagact ggaggtattg
481ctgagtggtt gaatggtttg gaatatgtgc cacaaagact gaaaaatctt aatgaaatta
541ataagctgct agcacatcga ccttggctga tcacaaaaga gcacattcag aaacttGTCA
601AAACTGGAGA AAATAATTGG TCTCTGCCTG AACTGGTACA TGCTGTGGTC CTCCTGGCAC
661ATTATCATGC TTTGGCAAGC TTTGTTTTTG GTAGTGGTAT CAATCCAGAG AGAGATCCAG
721AAATCTCCAA TGGATTCAGG CTAATATCAG TCAACAATTT CTGCGTTTGT GATCTTGCTA
781ATGACAACAA CATAGAGAAT GCATCTCTTT CAGGCAGCAA CTTTGGGATT GTGGATTCTC
841TAAGTGAGCT AGAGGCCTTA ATGGAAAGGA TGAAAAGACT TCAAGAAGAA AGGGAAGATG
901AAGAGGCGTC TCAAGAAGAA ATGAGCACTC GTTTTGAAAA GGAGAAGAAA GAAAGTCTTT
961TTGTGGTCTC TGGAGATACT TTTCATTCAT TTCCTCATTC AGGTGCTTTT TTGCACTTTT
1021TTGCCTTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1081CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1141TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGGAC TATCATGTAT GCTTCAACAC
1201GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)