Construct: ORF TRCN0000473047
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004812.1_s317c1
- Derived from:
- ccsbBroadEn_13218
- DNA Barcode:
- ATGGACTATCATGTATGCTTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SESN3 (143686)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473047
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143686 | SESN3 | sestrin 3 | NM_144665.4 | 64.1% | 62.7% | (many diffs) |
| 2 | human | 143686 | SESN3 | sestrin 3 | XM_011542614.1 | 59.2% | 57.7% | (many diffs) |
| 3 | human | 143686 | SESN3 | sestrin 3 | XM_024448355.1 | 48.3% | 47% | (many diffs) |
| 4 | human | 143686 | SESN3 | sestrin 3 | NM_001271594.2 | 36% | 34.8% | (many diffs) |
| 5 | mouse | 75747 | Sesn3 | sestrin 3 | NM_030261.4 | 57.6% | 57.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccggggcggc ggcagcccgt cggccgccgc caactacctg ctctgtacca 121 actgccggaa agtgctgcgg aaggataaaa gaatcagagt gtctcaaccc ttgacaagag 181 gaccaagtgc ctttattcca gagaaggaag ttgtccaagc aaacacagtg gatgaacgta 241 ctaactttct tgtggaagaa tactctacat ccggtcgtct ggacaacatc acacaggtca 301 tgagtttaca cactcagtac ctggagtctt tcttgcggag ccagttttac atgttgcgca 361 tggatggtcc ccttcctcta ccatacaggc actatattgc aataatggct gcagctagac 421 atcagtgttc ttacttaata aacatgcatg tggatgaatt tttaaagact ggaggtattg 481 ctgagtggtt gaatggtttg gaatatgtgc cacaaagact gaaaaatctt aatgaaatta 541 ataagctgct agcacatcga ccttggctga tcacaaaaga gcacattcag aaacttGTCA 601 AAACTGGAGA AAATAATTGG TCTCTGCCTG AACTGGTACA TGCTGTGGTC CTCCTGGCAC 661 ATTATCATGC TTTGGCAAGC TTTGTTTTTG GTAGTGGTAT CAATCCAGAG AGAGATCCAG 721 AAATCTCCAA TGGATTCAGG CTAATATCAG TCAACAATTT CTGCGTTTGT GATCTTGCTA 781 ATGACAACAA CATAGAGAAT GCATCTCTTT CAGGCAGCAA CTTTGGGATT GTGGATTCTC 841 TAAGTGAGCT AGAGGCCTTA ATGGAAAGGA TGAAAAGACT TCAAGAAGAA AGGGAAGATG 901 AAGAGGCGTC TCAAGAAGAA ATGAGCACTC GTTTTGAAAA GGAGAAGAAA GAAAGTCTTT 961 TTGTGGTCTC TGGAGATACT TTTCATTCAT TTCCTCATTC AGGTGCTTTT TTGCACTTTT 1021 TTGCCTTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGGAC TATCATGTAT GCTTCAACAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt