Transcript: Human NM_144665.4

Homo sapiens sestrin 3 (SESN3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-16
Taxon:
Homo sapiens (human)
Gene:
SESN3 (143686)
Length:
9563
CDS:
343..1821

Additional Resources:

NCBI RefSeq record:
NM_144665.4
NBCI Gene record:
SESN3 (143686)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_144665.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412760 ATCTGATGTCTCTCGATATAT pLKO_005 1305 CDS 100% 15.000 12.000 N SESN3 n/a
2 TRCN0000432269 GGTCTACAATCTCACATATAA pLKO_005 1485 CDS 100% 15.000 10.500 N SESN3 n/a
3 TRCN0000428761 GGCTAATATCAGTCAACAATT pLKO_005 1016 CDS 100% 13.200 9.240 N SESN3 n/a
4 TRCN0000088252 GCCTTAATGGAAAGGATGAAA pLKO.1 1132 CDS 100% 5.625 3.938 N Sesn3 n/a
5 TRCN0000141228 CCCATGAGGATGTTGACACAA pLKO.1 1517 CDS 100% 4.950 3.465 N SESN3 n/a
6 TRCN0000143446 GCTATCCTGAGAGAACTACAA pLKO.1 1667 CDS 100% 4.950 3.465 N SESN3 n/a
7 TRCN0000122213 GCTGAACTTCTTTATGCTCTT pLKO.1 1774 CDS 100% 4.050 2.835 N SESN3 n/a
8 TRCN0000144279 CGTACTAACTTTCTTGTGGAA pLKO.1 514 CDS 100% 2.640 1.848 N SESN3 n/a
9 TRCN0000144367 CAGTTCTCTAGTGTCAAAGTT pLKO.1 1914 3UTR 100% 5.625 3.375 N SESN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144665.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13218 pDONR223 100% 64.1% 62.7% None (many diffs) n/a
2 ccsbBroad304_13218 pLX_304 0% 64.1% 62.7% V5 (many diffs) n/a
3 TRCN0000473047 ATGGACTATCATGTATGCTTCAAC pLX_317 45% 64.1% 62.7% V5 (many diffs) n/a
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