Construct: ORF TRCN0000473067
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018846.2_s317c1
- Derived from:
- ccsbBroadEn_14888
- DNA Barcode:
- TGACGGGCAGCCGACGCTAGCAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BRD3 (8019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473067
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8019 | BRD3 | bromodomain containing 3 | XM_017015165.2 | 75% | 33.1% | (many diffs) |
| 2 | human | 8019 | BRD3 | bromodomain containing 3 | NM_007371.4 | 74.9% | 33% | (many diffs) |
| 3 | human | 8019 | BRD3 | bromodomain containing 3 | XM_006717291.3 | 74.9% | 33% | (many diffs) |
| 4 | human | 8019 | BRD3 | bromodomain containing 3 | XM_011519052.2 | 74.9% | 33% | (many diffs) |
| 5 | human | 8019 | BRD3 | bromodomain containing 3 | XM_017015166.2 | 31.1% | 13.8% | (many diffs) |
| 6 | mouse | 67382 | Brd3 | bromodomain containing 3 | NM_001113573.1 | 66.5% | 31.6% | (many diffs) |
| 7 | mouse | 67382 | Brd3 | bromodomain containing 3 | NM_023336.4 | 66.5% | 31.6% | (many diffs) |
| 8 | mouse | 67382 | Brd3 | bromodomain containing 3 | NM_001113574.1 | 65% | 30.9% | (many diffs) |
| 9 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_006498249.3 | 65% | 30.9% | (many diffs) |
| 10 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_006498250.2 | 65% | 30.9% | (many diffs) |
| 11 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_011239155.2 | 65% | 30.9% | (many diffs) |
| 12 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_011239156.2 | 65% | 30.9% | (many diffs) |
| 13 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_011239157.2 | 65% | 30.9% | (many diffs) |
| 14 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_011239158.2 | 65% | 30.9% | (many diffs) |
| 15 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_017319221.1 | 28.1% | 13.5% | (many diffs) |
| 16 | mouse | 67382 | Brd3 | bromodomain containing 3 | XM_011239159.2 | 27.4% | 13.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 792
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccaccgcc acgacagtcg cccccgcggg gatcccggcg accccgggcc 121 ctgtgaaccc accccccccg gaggtctcca accccagcaa gcccggccgc aagaccaacc 181 agctgcagta catgcagaat gtggtggtga agacgctctg gaaacaccag ttcgcctggc 241 ccttctacca gcccgtggac gcaatcaaat tgaacctgcc ggattatcat aaaataatta 301 aaaacccaat ggatatgggg actattaaga agagactaga aaataattat tattggagtg 361 caagcgaatg tatgcaggac ttcaacacca tgtttacaaa ttgttacatt tataacaagc 421 ccacagatga catagtgcta atggcccaag ctttagagaa aatttttcta caaaaagtgg 481 cccagatgcc ccaagaggaa gttgaattat taccccctgc tccaaagggc aaaggtcgga 541 agccggctgc gggagcccag agcgcaggta cacagcaagt ggcggccgtg tcctctgtct 601 ccccagcgac cccctttcag agcgtgcccc ccaccgtctc ccagacgccc gtcatcgctg 661 ccacccctgt accaaccatc actgcaaacg tcacgtcggt cccagtcccc ccagctgccg 721 ccccacctcc tcctgccaca cccatcgtcc ccgtggtccc tcctacgccg cctgtcgtca 781 agaaaaaggc gtgaagcgga aagcagacac aaccactccc acgacgtcgg ccatcactgc 841 cagccggagt gagtcgcccc cgccgttgtc agaccccaag caggccaaag tggtggcccg 901 gcgggagagt ggtggccgcc ccatcaagcc tcccaagaag gacctggaga acggccgagg 961 tgccccagca cgccaggcaa gaagggcaag ctgtcggagc acctgcgcta ctgcgacagc 1021 atcctcaggg agatgctatc caagaagcac gcggctacgc ctggccttct acaagccagt 1081 ggatgccgag ggcctggagc tgcacgacta ccacgacatc atcaagcacc cgatggacct 1141 cagcaccgtg aaaaggaaga tggatggccg agagtaccca gacgcacagg gctttgctgc 1201 tgatgtccgg ctgatgttct cgaattgcta caaatacaat cccccagacc acgaggttgt 1261 ggccatggcc cggaagctcc aggacgtgtt tgagatgagg tttgccaaga tgccagatga 1321 gcccgtggag gcaccggcgc tgcctgcccc tgcggccccc atggtgagca agggcgctga 1381 gagcagccgt agcagtgagg agagctcttc ggacTCAGGC AGCTCGGACT CGGAGGAGGA 1441 GCGGGCCACC AGGCTGGCGG AGCTGCAGGA GCAGCTGAAG GCCGTGCACG AGCAGCTGGC 1501 CGCCCTGTCT CAGGCCCCAG TAAACAAACC AAAGAAGAAG AAGGAGAAGA AGGAGAAGGA 1561 GAAGAAGAAG AAGGACAAGG AGAAGGAGAA GGAGAAGCAC AAAGTGAAGG CCGAGGAAGA 1621 GAAGAAGGCC AAGGTGGCTC CGCCTGCCAA GCAGGCTCAG CAGAAGAAGG CTCCTGCCAA 1681 GAAGGCCAAC AGCACGACCA CGGCCGGCAG AGATCATTTC TTGACCTGTG GAGTTTTGCC 1741 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 1801 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 1861 TATATCTTGT GGAAAGGACG ATGACGGGCA GCCGACGCTA GCAGTACGCG TTAAGTCgac 1921 aatcaacctc tggattacaa aatttgtgaa agatt