Construct: ORF TRCN0000473073
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007816.1_s317c1
- Derived from:
- ccsbBroadEn_01008
- DNA Barcode:
- TGACACCGGCCCATAGAATGACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KITLG (4254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473073
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4254 | KITLG | KIT ligand | NM_000899.5 | 100% | 100% | |
| 2 | human | 4254 | KITLG | KIT ligand | NM_003994.5 | 89.7% | 89.3% | 520_521ins84 |
| 3 | mouse | 17311 | Kitl | kit ligand | NM_013598.3 | 89.2% | 82.7% | (many diffs) |
| 4 | mouse | 17311 | Kitl | kit ligand | NM_001347156.1 | 79% | 72.1% | (many diffs) |
| 5 | mouse | 17311 | Kitl | kit ligand | XM_006513314.3 | 77.9% | 72.2% | (many diffs) |
| 6 | mouse | 17311 | Kitl | kit ligand | XM_006513315.2 | 69% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaagacacaa acttggattc tcacttgcat ttatcttcag ctgctcctat 121 ttaatcctct cgtcaaaact gaagggatct gcaggaatcg tgtgactaat aatgtaaaag 181 acgtcactaa attggtggca aatcttccaa aagactacat gataaccctc aaatatgtcc 241 ccgggatgga tgttttgcca agtcattgtt ggataagcga gatggtagta caattgtcag 301 acagcttgac tgatcttctg gacaagtttt caaatatttc tgaaggcttg agtaattatt 361 ccatcataga caaacttgtg aatatagtgg atgaccttgt ggagtgcgtg aaagaaaact 421 catctaagga tctaaaaaaa tcattcaaga gcccagaacc caggctcttt actccTGAAG 481 AATTCTTTAG AATTTTTAAT AGATCCATTG ATGCCTTCAA GGACTTTGTA GTGGCATCTG 541 AAACTAGTGA TTGTGTGGTT TCTTCAACAT TAAGTCCTGA GAAAGATTCC AGAGTCAGTG 601 TCACAAAACC ATTTATGTTA CCCCCTGTTG CAGCCAGCTC CCTTAGGAAT GACAGCAGTA 661 GCAGTAATAG GAAGGCCAAA AATCCCCCTG GAGACTCCAG CCTACACTGG GCAGCCATGG 721 CATTGCCAGC ATTGTTTTCT CTTATAATTG GCTTTGCTTT TGGAGCCTTA TACTGGAAGA 781 AGAGACAGCC AAGTCTTACA AGGGCAGTTG AAAATATACA AATTAATGAA GAGGATAATG 841 AGATAAGTAT GTTGCAAGAG AAAGAGAGAG AGTTTCAAGA AGTGTACCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA TGACACCGGC CCATAGAATG ACCGACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt