Transcript: Human NM_003994.5

Homo sapiens KIT ligand (KITLG), transcript variant a, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
KITLG (4254)
Length:
5376
CDS:
196..933

Additional Resources:

NCBI RefSeq record:
NM_003994.5
NBCI Gene record:
KITLG (4254)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003994.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000146635 CCTATTTAATCCTCTCGTCAA pLKO.1 246 CDS 100% 4.050 5.670 N KITLG n/a
2 TRCN0000149952 GTCTTACAAGGGCAGTTGAAA pLKO.1 839 CDS 100% 5.625 4.500 N KITLG n/a
3 TRCN0000059232 CCAAGTCTTACAAGGGCAGTT pLKO.1 835 CDS 100% 4.050 3.240 N KITLG n/a
4 TRCN0000059229 CGTCACTAAATTGGTGGCAAA pLKO.1 312 CDS 100% 4.050 3.240 N KITLG n/a
5 TRCN0000149589 CGTCACTAAATTGGTGGCAAA pLKO.1 312 CDS 100% 4.050 3.240 N KITLG n/a
6 TRCN0000059231 GCAGGAATCGTGTGACTAATA pLKO.1 281 CDS 100% 13.200 9.240 N KITLG n/a
7 TRCN0000149884 GCAGGAATCGTGTGACTAATA pLKO.1 281 CDS 100% 13.200 9.240 N KITLG n/a
8 TRCN0000148186 GACTACATGATAACCCTCAAA pLKO.1 343 CDS 100% 4.950 3.465 N KITLG n/a
9 TRCN0000150221 GAGAGAGTTTCAAGAAGTGTA pLKO.1 912 CDS 100% 4.950 3.465 N KITLG n/a
10 TRCN0000148375 CTTGACTGATCTTCTGGACAA pLKO.1 435 CDS 100% 4.050 2.835 N KITLG n/a
11 TRCN0000149199 GCTGCTCCTATTTAATCCTCT pLKO.1 240 CDS 100% 2.640 1.848 N KITLG n/a
12 TRCN0000150183 GTGAATATAGTGGATGACCTT pLKO.1 508 CDS 100% 2.640 1.848 N KITLG n/a
13 TRCN0000059228 GCTTCATAAATGAAGCAGCTT pLKO.1 991 3UTR 100% 0.264 0.185 N KITLG n/a
14 TRCN0000147684 GCTTCATAAATGAAGCAGCTT pLKO.1 991 3UTR 100% 0.264 0.185 N KITLG n/a
15 TRCN0000067872 GCCTTATACTGGAAGAAGAAA pLKO.1 811 CDS 100% 5.625 3.375 N Kitl n/a
16 TRCN0000303181 GCCTTATACTGGAAGAAGAAA pLKO_005 811 CDS 100% 5.625 3.375 N Kitl n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003994.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01008 pDONR223 100% 89.7% 89.3% None 520_521ins84 n/a
2 ccsbBroad304_01008 pLX_304 0% 89.7% 89.3% V5 520_521ins84 n/a
3 TRCN0000473073 TGACACCGGCCCATAGAATGACCG pLX_317 50% 89.7% 89.3% V5 520_521ins84 n/a
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