Construct: ORF TRCN0000473074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006341.1_s317c1
- Derived from:
- ccsbBroadEn_13626
- DNA Barcode:
- GTCGCCGGGCCAGAGCTTCTTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC100287896 (100287896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473074
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100287896 | LOC100287896 | uncharacterized LOC100287896 | NM_001319240.1 | 76% | 76% | 1_282del |
| 2 | human | 100287896 | LOC100287896 | uncharacterized LOC100287896 | XM_017017055.1 | 76% | 76% | 1_282del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac caccatatat gacctgaaga aacagaagga taaactgttg aagttttatg 121 ctgaaagtga tgagcagata ttaatgaaaa atagaaaaac acttcataaa gctaaaaatg 181 aagatcttga tcgtgtattg aaagagtgga tccgtcagcg tcgcagtgaa cacatgccac 241 ttaatggtat gctgatcatg aaacaagcaa agatatatca caatgaacta aaaattgagg 301 ggaactgtga atattcaaca ggctggttgc agaaatttaa gaaaagacat ggcattaaat 361 ttttaaagac ttgtggcaat aaagcatctg ctggtcatga agcaacagag aagtttactg 421 gcaagttcag taatgatgat gaacaagatg gtaactttga aggattcagt atgtcaagtg 481 agaaaaaaat aatgtctgac ctccttacat atacaaaaaa tatacatcca gagactgtca 541 gtaagctgga agaagaggat atcaaagatg tttttaacag taaTAATGAG GCTCCAGTTG 601 TTCATTCATT GTCCAATGGT GAAGTAACAA AAATGGTTCT GAATCAAGAT GATCATGATG 661 ATAATGATAA TGAAGATGAT GTTAACACTG CAGAAAAAGT GCCTATAGAC GACATGGTAA 721 AAATGTGTGA TGGGCTTATT AAAGGACTAG AGCAGCATGC ATTCATAACA GAGCAAGAAA 781 TCATGTCAGT TTATAAAATC AAAGAGAGAC TTCTAAGACA AAAAGCATCA TTAATGAGGC 841 AGATGACTCT GAAAGAAACA TTTAAAAAAG CCATCCAGAG GAATGCTTCT TCCTCTCTAC 901 AGGACCCACT TCTTGGTCCC TCAACTGCTT CTGATGCTTC TTCTCACCTA AAAATAAAAT 961 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAGTCGC CGGGCCAGAG CTTCTTCTTA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt