Construct: ORF TRCN0000473077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017647.1_s317c1
- Derived from:
- ccsbBroadEn_03793
- DNA Barcode:
- TTAAATCCGCAGGACACTGCGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTN4 (57142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57142 | RTN4 | reticulon 4 | NM_207520.2 | 100% | 100% | |
| 2 | human | 57142 | RTN4 | reticulon 4 | NM_153828.3 | 95.1% | 95.1% | 555_556ins57 |
| 3 | human | 57142 | RTN4 | reticulon 4 | NM_007008.3 | 49.1% | 47.9% | (many diffs) |
| 4 | human | 57142 | RTN4 | reticulon 4 | NM_020532.5 | 32.8% | 32.8% | 611_3010del |
| 5 | human | 57142 | RTN4 | reticulon 4 | NR_135829.1 | 29.7% | (many diffs) | |
| 6 | human | 57142 | RTN4 | reticulon 4 | NR_135830.2 | 28.2% | (many diffs) | |
| 7 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194053.3 | 85.4% | 87.7% | (many diffs) |
| 8 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194052.3 | 80.7% | 83.2% | (many diffs) |
| 9 | mouse | 68585 | Rtn4 | reticulon 4 | NM_024226.4 | 46.5% | 47.1% | (many diffs) |
| 10 | mouse | 68585 | Rtn4 | reticulon 4 | NM_194054.3 | 28.4% | 29.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1242
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agacctggac cagtctcctc tggtctcgtc ctcggacagc ccaccccggc 121 cgcagcccgc gttcaagtac cagttcgtga gggagcccga ggacgaggag gaagaagagg 181 aggaggaaga ggaggacgag gacgaagacc tggaggagct ggaggtgctg gagaggaagc 241 ccgccgccgg gctgtccgcg gccccagtgc ccaccgcccc tgccgccggc gcgcccctga 301 tggacttcgg aaatgacttc gtgccgccgg cgccccgggg acccctgccg gccgctcccc 361 ccgtcgcccc ggagcggcag ccgtcttggg acccgagccc ggtgtcgtcg accgtgcccg 421 cgccatcccc gctgtctgct gccgcagtct cgccctccaa gctccctgag gacgacgagc 481 ctccggcccg gcctccccct cctcccccgg ccagcgtgag cccccaggca gagcccgtgt 541 ggaccccgcc agccccggct cccgccgcgc ccccctccac cccggccgcg cccaagcgca 601 ggggctcctc gggctcagtg gatgagaccc tttttgctct tcctgctgca tctgagcctg 661 tgatacgctc ctctgcagtt gttgacctcc tgtactggag agacattaag aagactggag 721 tggtgtttgg tgccagccta ttcctgctgc tttcattgac agtattcagc attgtgagcg 781 taacagccta cattgccttg gcccTGCTCT CTGTGACCAT CAGCTTTAGG ATATACAAGG 841 GTGTGATCCA AGCTATCCAG AAATCAGATG AAGGCCACCC ATTCAGGGCA TATCTGGAAT 901 CTGAAGTTGC TATATCTGAG GAGTTGGTTC AGAAGTACAG TAATTCTGCT CTTGGTCATG 961 TGAACTGCAC GATAAAGGAA CTCAGGCGCC TCTTCTTAGT TGATGATTTA GTTGATTCTC 1021 TGAAGTTTGC AGTGTTGATG TGGGTATTTA CCTATGTTGG TGCCTTGTTT AATGGTCTGA 1081 CACTACTGAT TTTGGCTCTC ATTTCACTCT TCAGTGTTCC TGTTATTTAT GAACGGCATC 1141 AGGCACAGAT AGATCATTAT CTAGGACTTG CAAATAAGAA TGTTAAAGAT GCTATGGCTA 1201 AAATCCAAGC AAAAATCCCT GGATTGAAGC GCAAAGCTGA ATGCCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGATTA AATCCGCAGG ACACTGCGCT GACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t