Construct: ORF TRCN0000473091
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004392.1_s317c1
- Derived from:
- ccsbBroadEn_04968
- DNA Barcode:
- TTACTACCAGTTTAATTAACAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRICKLE1 (144165)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473091
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | NM_001144881.2 | 100% | 100% | |
| 2 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | NM_001144882.2 | 100% | 100% | |
| 3 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | NM_001144883.2 | 100% | 100% | |
| 4 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | NM_153026.3 | 100% | 100% | |
| 5 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | XM_011537946.1 | 100% | 100% | |
| 6 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | XM_011537947.2 | 100% | 100% | |
| 7 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | XM_017018838.1 | 100% | 100% | |
| 8 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | XM_017018839.1 | 100% | 100% | |
| 9 | human | 144165 | PRICKLE1 | prickle planar cell polarit... | XM_017018840.1 | 100% | 100% | |
| 10 | mouse | 106042 | Prickle1 | prickle planar cell polarit... | NM_001033217.4 | 84.8% | 93.1% | (many diffs) |
| 11 | mouse | 106042 | Prickle1 | prickle planar cell polarit... | XM_006520264.3 | 84.8% | 93.1% | (many diffs) |
| 12 | mouse | 106042 | Prickle1 | prickle planar cell polarit... | XM_006520265.1 | 84.8% | 93.1% | (many diffs) |
| 13 | mouse | 106042 | Prickle1 | prickle planar cell polarit... | XM_006520266.3 | 84.8% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 68
- ORF end:
- 2561
- ORF length:
- 2493
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcacatg cctttggaga tggagcccaa gatgagcaaa ctggcctttg gctgtcagag 121 aagttccaca tcagatgatg actctggctg tgcattggag gagtacgcct gggtcccccc 181 gggcctgaga ccagagcaga tccagctcta ttttgcttgc ttaccagagg aaaaagttcc 241 ttacgttaac agccccggag agaagcatcg gattaaacag cttttgtacc agttaccacc 301 acatgataat gaggtacggt attgccagtc tttgagtgaa gaggagaaaa aagagttgca 361 ggtgttcagt gctcagcgga agaaagaagc actgggaaga ggaacaatta agcttctgtc 421 cagagcagtc atgcatgctg tgtgtgagca gtgtggtttg aagataaatg gaggtgaagt 481 tgcagtgttc gcctcccgtg cgggccctgg tgtgtgctgg cacccatcct gttttgtctg 541 tttcacgtgt aatgagctgc tggtcgacct catctatttt tatcaggatg gaaaaattca 601 ctgtggcagg caccatgcag aactgctcaa accacggtgc tcagcatgtg acgagataat 661 ttttgctgat gagtgcacag aagctgaggg tcgccattgg cacatgaaac acttctgctg 721 ccttgagtgt gaaacggtcc tgggaggaca gaggtatatc atgaaggacg gccgcccctt 781 ctgctgtggc tgttttgagt ctctctatgc ggagtactgt gaaacctgtg gggaacatat 841 tggtgtggac catgcacaga tgacctatga cgggcagcac tggcacgcca cggaagcctg 901 cttttcttgt gcccagtgta aagcctcttt gttgggatgt cccttccttc ccaaacaggg 961 tcagatttac tgctcaaaaa cgtgcagtct tggtgaagac gtccatgcct ctgattcttc 1021 cgactctgca tttcagtcag ctcgatcaag agactcccga agaagtgtcc gaatgggcaa 1081 gagcagccgg tcagcagatc agtgtagaca gtctctcctc ttatcgcctg ctctgaacta 1141 caagtttcct ggcctctcag gcaatgctga tgacaccctt tctcgaaaat tggatgatct 1201 gagtctctcc agacaaggaa caagttttgc cagtgaagaa ttttggaaag gcagagtaga 1261 gcaggaaact ccagaagacc ctgaagaatg ggctgatcat gaagattata tgacgcagct 1321 cctcctcaag tttggtgata aaagcctctt tcagccacag cccaatgaga tggatattcg 1381 agccagtgag cactggatat ctgataacat ggttaaaagt aagaccgagt taaagcaaaa 1441 taaccagagc cttgcaagta aaaaatacca gtctgatatg tactgggcac agtcacaaga 1501 tggactgggc gattctgctt atggcagcca cccaggccct gcaagcagta gaaggcttca 1561 ggaattggaa ctggaccatg gggcttcagg gtataatcat gatgaaacac agtggtatga 1621 agattccctg gagtgtctgt cagacctgaa accagagcaa agtgttcggg attcgatgga 1681 ttctttggca ttgtccaata tcacaggggc ttcggtggat ggagaaaaca agccaaggcc 1741 atcattgtat tctctgcaaa attttgagga gatggaaaca gaagattgtg agaagatgag 1801 caatatggga actttgaact cttccatgct gcacaggagt gcagagtcct taaagagtct 1861 aagttcagag ttgtgtccag agaaaatcct gcctgaagag aagccagtac atctgccagt 1921 gctcagaagg tccaagtctc aatccagacc ccagcaggtc aagttttctg atgatgtcat 1981 tgacaatggg aactatgaca ttgaaatccg gcagcctccg atgagtgaaa ggactcggag 2041 acgcgtctac aattttgaag agaggggatc caggtctcat caccaccgcc gccggagaag 2101 tagaaagtcc cgctccgaca atgccctGAA TCTTGTTACA GAAAGAAAAT ACTCTCCCAA 2161 GGACAGACTG CGGCTGTACA CCCCCGATAA CTATGAGAAA TTTATACAGA ATAAAAGTGC 2221 CCGGGAGATC CAAGCATACA TCCAGAATGC TGATCTCTAC GGACAGTACG CCCATGCCAC 2281 TTCCGATTAT GGCCTGCAGA ACCCAGGAAT GAATCGGTTT CTGGGACTCT ACGGCGAGGA 2341 TGATGATTCC TGGTGTTCTT CCTCCTCCTC CTCTTCCGAC TCGGAAGAAG AAGGATATTT 2401 TCTTGGACAA CCAATCCCTC AACCCCGGCC ACAGAGATTT GCCTACTATA CAGATGACCT 2461 TTCTAGTCCA CCATCTGCAC TTCCCACCCC TCAGTTTGGT CAGAGGACAA CAAAATCCAA 2521 GAAGAAAAAG GGACACAAGG GCAAAAATTG TATTATTTCT TTGCCAACTT TCTTGTACAA 2581 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 2641 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 2701 GGACGATTAC TACCAGTTTA ATTAACAGAT ACGCGTTAAG TCgacaatca acctctggat 2761 tacaaaattt gtgaaagatt