Construct: ORF TRCN0000473096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008608.1_s317c1
- Derived from:
- ccsbBroadEn_04908
- DNA Barcode:
- CGTTCCGGTTGCCCGATATTTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASZ1 (136991)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473096
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 136991 | ASZ1 | ankyrin repeat, SAM and bas... | NM_130768.3 | 100% | 100% | |
| 2 | human | 136991 | ASZ1 | ankyrin repeat, SAM and bas... | NM_001301821.2 | 98.1% | 98.1% | 1159_1160ins27 |
| 3 | human | 136991 | ASZ1 | ankyrin repeat, SAM and bas... | NM_001301822.2 | 56.2% | 56.2% | 0_1ins624 |
| 4 | mouse | 74068 | Asz1 | ankyrin repeat, SAM and bas... | NM_023729.3 | 85.9% | 85.2% | (many diffs) |
| 5 | mouse | 74068 | Asz1 | ankyrin repeat, SAM and bas... | XM_006505176.3 | 59.2% | 59.1% | (many diffs) |
| 6 | mouse | 74068 | Asz1 | ankyrin repeat, SAM and bas... | XM_006505178.3 | 59.2% | 59.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1494
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgagc gcgctgcgag gcctgccagt ggctggcgga ggcgagagta 121 gcgagagcga ggatgatggc tgggagattg ggtatctcga ccggacgtct cagaaattga 181 aaaggctatt acccattgaa gaaaagaaag aaaaatttaa gaaagcaatg accatcggag 241 atgtttcatt ggtccaggag ctcctagatt ctggcattag tgtagattcc aactttcagt 301 atggatggac tccccttatg tatgctgcta gtgttgccaa tgcagagctg gttcgggtcc 361 ttttggacag aggtgctaat gcaagctttg agaaggataa gcaaagtatt ttgataactg 421 catgttctgc tcatggctca gaggaacaga tcttgaagtg tgtagaacta ctactttcaa 481 gaaatgctga tccaaatgtt gcttgtagga gacttatgac cccaatcatg tatgctgctc 541 gagatggtca cacccaggtt gttgctctcc ttgttgctca tggagcagaa gttaataccc 601 aggatgagaa tggttacact gctttaacgt gggcagcacg tcagggtcat aaaaatatag 661 ttttgaagtt gcttgaactt ggagctaata aaatgctaca aaccaaagat ggaaagatgc 721 caagtgagat tgcaaaaaga aacaaacatc atgagatctt caacttactt tcttttactt 781 taaatccatt ggaaggaaaa cttcaacagc taactaaaga agacactatt tgtaaaatat 841 tgacaacaga ttctgataga gaaaaagatc acatttttag ttcatataca gcatttggag 901 atctggaagt atttttacat ggtcttggac ttgaacatat gacagattta ctaaaggaaa 961 gggatataac gttaagacat cttttgaCCA TGAGGGAAGA TGAATTTACA AAGAATGGAA 1021 TTACCAGTAA AGACCAGCAG AAAATTCTGG CTGCTCTTAA AGAACTACAG GTAGAAGAGA 1081 TACAATTTGG AGAGCTATCT GAAGAGACAA AGTTGGAAAT CAGTGGTGAT GAGTTCCTCA 1141 ACTTTCTTCT CAAATTAAAT AAACAGTGTG GCCATTTAAT AACAGCTGTA CAGAATGTTA 1201 TTACTGAGTT ACCTGTAAAT TCTCAAAAGA TAACACTGGA ATGGGCTTCT CCCCAGAATT 1261 TTACTTCAGT TTGTGAAGAA TTGGTTAATA ATGTTGAAGA TTTGAGTGAA AAGGTCTGCA 1321 AACTAAAAGA CCTAATTCAA AAGTTGCAAA ATGAACGGGA AAATGATCCA ACTCATATAC 1381 AATTAAGGGA AGAAGTATCT ACATGGAATA GTAGAATTTT GAAGAGGACA GCTATTACCA 1441 TATGCGGATT CGGTTTTCTT CTTTTCATTT GCAAGCTAAC TTTCCAGAGG AAATTGCCAA 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAC GTTCCGGTTG CCCGATATTT GGAACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att