Transcript: Human NM_001301822.2

Homo sapiens ankyrin repeat, SAM and basic leucine zipper domain containing 1 (ASZ1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-06-01
Taxon:
Homo sapiens (human)
Gene:
ASZ1 (136991)
Length:
1723
CDS:
546..1349

Additional Resources:

NCBI RefSeq record:
NM_001301822.2
NBCI Gene record:
ASZ1 (136991)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001301822.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414728 ACTGTTTGCAGCACAATATAA pLKO_005 1415 3UTR 100% 15.000 10.500 N ASZ1 n/a
2 TRCN0000423563 AGAGGACAGCTATTACCATAT pLKO_005 1276 CDS 100% 10.800 7.560 N ASZ1 n/a
3 TRCN0000128986 GAACAGATCTTGAAGTGTGTA pLKO.1 409 5UTR 100% 4.950 3.465 N ASZ1 n/a
4 TRCN0000131236 GCAATGACCATCGGAGATGTT pLKO.1 190 5UTR 100% 4.950 3.465 N ASZ1 n/a
5 TRCN0000127928 CAAGCTAACTTTCCAGAGGAA pLKO.1 1325 CDS 100% 2.640 1.848 N ASZ1 n/a
6 TRCN0000130485 GAGATACAATTTGGAGAGCTA pLKO.1 930 CDS 100% 2.640 1.848 N ASZ1 n/a
7 TRCN0000129402 GCAAGCTAACTTTCCAGAGGA pLKO.1 1324 CDS 100% 2.640 1.848 N ASZ1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001301822.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04908 pDONR223 100% 56.2% 56.2% None 0_1ins624 n/a
2 ccsbBroad304_04908 pLX_304 0% 56.2% 56.2% V5 0_1ins624 n/a
3 TRCN0000473096 CGTTCCGGTTGCCCGATATTTGGA pLX_317 35.7% 56.2% 56.2% V5 0_1ins624 n/a
4 ccsbBroadEn_10548 pDONR223 100% 38.5% 37.6% None (many diffs) n/a
5 ccsbBroad304_10548 pLX_304 0% 38.5% 37.6% V5 (many diffs) n/a
6 TRCN0000491826 AACGGTTTTCCCCCTAGATCGGAA pLX_317 11.4% 38.5% 37.6% V5 (many diffs) n/a
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