Construct: ORF TRCN0000473116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002839.1_s317c1
- Derived from:
- ccsbBroadEn_07539
- DNA Barcode:
- TTCTGCAATGCAATCGGTGTTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBA2 (10054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10054 | UBA2 | ubiquitin like modifier act... | NM_005499.3 | 99.9% | 100% | 1776A>G |
| 2 | human | 10054 | UBA2 | ubiquitin like modifier act... | XM_006722962.2 | 95.5% | 95.6% | 137_138ins84;1692A>G |
| 3 | human | 10054 | UBA2 | ubiquitin like modifier act... | XM_024451305.1 | 91.8% | 91.8% | 1245_1246ins156;1620A>G |
| 4 | human | 10054 | UBA2 | ubiquitin like modifier act... | XM_005258404.3 | 85% | 78.5% | 1_242del;380_473del;2112A>G |
| 5 | human | 10054 | UBA2 | ubiquitin like modifier act... | XM_011526304.2 | 78.1% | 71.8% | (many diffs) |
| 6 | human | 10054 | UBA2 | ubiquitin like modifier act... | XR_001753571.1 | 68.3% | (many diffs) | |
| 7 | human | 10054 | UBA2 | ubiquitin like modifier act... | XM_017026134.1 | 65.9% | 56% | (many diffs) |
| 8 | human | 10054 | UBA2 | ubiquitin like modifier act... | XR_935712.2 | 61.8% | (many diffs) | |
| 9 | mouse | 50995 | Uba2 | ubiquitin-like modifier act... | NM_016682.2 | 87.6% | 94.3% | (many diffs) |
| 10 | mouse | 50995 | Uba2 | ubiquitin-like modifier act... | XM_017322348.1 | 81.2% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc actgtcgcgg gggctgcccc gggagctggc tgaggcggtg gccgggggcc 121 gggtgctggt ggtgggggcg ggcggcatcg gctgcgagct cctcaagaat ctcgtgctca 181 ccggtttctc ccacatcgac ctgattgatc tggatactat tgatgtaagc aacctcaaca 241 gacagttttt gtttcaaaag aaacatgttg gaagatcaaa ggcacaggtt gccaaggaaa 301 gtgtactgca gttttacccg aaagctaata tcgttgccta ccatgacagc atcatgaacc 361 ctgactataa tgtggaattt ttccgacagt ttatactggt tatgaatgct ttagataaca 421 gagctgcccg aaaccatgtt aatagaatgt gcctggcagc tgatgttcct cttattgaaa 481 gtggaacagc tgggtatctt ggacaagtaa ctactatcaa aaagggtgtg accgagtgtt 541 atgagtgtca tcctaagccg acccagagaa cctttcctgg ctgtacaatt cgtaacacac 601 cttcagaacc tatacattgc atcgtttggg caaagtactt gttcaaccag ttgtttgggg 661 aagaagatgc tgatcaagaa gtatctcctg acagagctga ccctgaagct gcctgggaac 721 caacggaagc cgaagccaga gctagagcat ctaatgaaga tggtgacatt aaacgtattt 781 ctactaagga atgggctaaa tcaactggat atgatccagt taaacttttt accaagcttt 841 ttaaagatga catcaggtat ctgttgacaa tggacaaact atggcggaaa aggaaacctc 901 cagttccgtt ggactgggct gaagtacaaa gtcaaggaga agaaacgaat gcatcagatc 961 aacagaatga accccagtta ggcctgaaag accagcaggt tctagatgta aagagctatg 1021 cacgtctttt ttcaaagagc atcgagactt tgagagttca tttagcagaa aagggggatg 1081 gagctgagct catatgggat aaggatgacc catctgcaat ggattttgtc acctctgctg 1141 caaacctcag gatgcatatt ttcagtatga atatgaagag tagatttgat atcaaatcaa 1201 tggcagggaa cattattcct gctattgcta ctactaatgc agtaattgct gggttgatag 1261 tattggaagg attgaagatt ttatcaggaa aaatagacca gtgcagaaca atttttttga 1321 ataaacaacc aaacccaaga aagaagcttc ttgtgccttg tgcactggat cctcccaacc 1381 ccaattgtta tgtatgtgcc agcaagccag aggtgactgt gcggctgaat gtccataaag 1441 tgactgttct caccttacaa gacaagatag tgaaagaaaa atttgctatg gtagcaccag 1501 atgtccaaat tgaagatggg aaaggaacaa tcctaatatc ttccgaagag ggagagacgg 1561 aagctaataa tcacaagaag ttgtcagaat ttggaattag aaatggcagc cggcttcaag 1621 cagatgactt ccTCCAGGAC TATACTTTAT TGATCAACAT CCTTCATAGT GAAGACCTAG 1681 GAAAGGACGT TGAATTTGAA GTTGTTGGTG ATGCCCCGGA AAAAGTGGGG CCCAAACAAG 1741 CTGAAGATGC TGCCAAAAGC ATAACCAATG GCAGTGATGA TGGAGCTCAG CCCTCCACCT 1801 CCACAGCTCA AGAGCAAGAT GACGTTCTCA TAGTTGATTC GGATGAAGAA GATTCTTCAA 1861 ATAATGCCGA CGTCAGTGAA GAAGAGAGAA GCCGCAAGAG GAAATTAGAT GAGAAAGAGA 1921 ATCTCAGTGC AAAGAGGTCA CGTATAGAAC AGAAGGAAGA GCTTGATGAT GTCATAGCAT 1981 TAGATTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2041 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2101 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTCTGCAAT GCAATCGGTG TTCCAACGCG 2161 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt