Construct: ORF TRCN0000473120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010151.1_s317c1
- Derived from:
- ccsbBroadEn_06769
- DNA Barcode:
- TGATGACTGGCATGTACAAGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRIM2 (5558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5558 | PRIM2 | DNA primase subunit 2 | NM_000947.5 | 99.9% | 99.8% | 870G>T |
| 2 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_017011004.2 | 88.7% | 88.6% | 693_884del;1062G>T |
| 3 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_017011005.2 | 72.8% | 71.9% | (many diffs) |
| 4 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_017011006.2 | 59.3% | 59.1% | (many diffs) |
| 5 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_011514731.3 | 56.8% | 55% | (many diffs) |
| 6 | human | 5558 | PRIM2 | DNA primase subunit 2 | XM_024446483.1 | 52.3% | 50.3% | 0_1ins658;35_36ins68;144G>T |
| 7 | human | 101930420 | LOC101930420 | DNA primase large subunit-like | XM_017007609.2 | 45.3% | 35.7% | (many diffs) |
| 8 | human | 101930420 | LOC101930420 | DNA primase large subunit-like | XM_017030301.2 | 45.3% | 35.7% | (many diffs) |
| 9 | human | 5558 | PRIM2 | DNA primase subunit 2 | NM_001282487.2 | 30.8% | 30.2% | (many diffs) |
| 10 | human | 5558 | PRIM2 | DNA primase subunit 2 | NM_001282488.1 | 30.8% | 30.2% | (many diffs) |
| 11 | human | 107984021 | LOC107984021 | uncharacterized LOC107984021 | XR_001740805.2 | 28.4% | (many diffs) | |
| 12 | human | 107984021 | LOC107984021 | uncharacterized LOC107984021 | XR_001756820.2 | 28.4% | (many diffs) | |
| 13 | mouse | 19076 | Prim2 | DNA primase, p58 subunit | NM_008922.2 | 83.7% | 89.1% | (many diffs) |
| 14 | mouse | 19076 | Prim2 | DNA primase, p58 subunit | XM_011238455.2 | 48.7% | 48% | (many diffs) |
| 15 | mouse | 19076 | Prim2 | DNA primase, p58 subunit | XM_006495797.2 | 37.8% | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttttctgga agaaagtgga ggaagctgag gttggcaggt gaccagagga 121 atgcttccta ccctcattgc cttcagtttt acttgcagcc accttctgaa aacatatctt 181 taatagaatt tgaaaacttg gctattgata gagttaaatt gttaaaatca gttgaaaatc 241 ttggagtgag ctatgtgaaa ggaactgaac aataccagag taagttggag agtgagcttc 301 ggaagctcaa gttttcctac agagaaaact tagaagatga atatgaacca cgaagaagag 361 atcatatttc tcattttatt ttgcggcttg cttattgcca gtctgaagaa cttagacgct 421 ggttcattca acaagaaatg gatctccttc gatttagatt tagtatttta cccaaggata 481 aaattcagga tttcttaaag gatagccaat tgcagtttga ggctataagt gatgaagaga 541 agactcttcg agaacaggag attgttgcct catcaccaag tttaagtgga cttaagttgg 601 ggttcgagtc catttataag atcccttttg ctgatgctct ggatttgttt cgaggaagga 661 aagtctattt ggaagatggc tttgcttacg taccacttaa ggacattgtg gcaatcatcc 721 tgaatgaatt tagagccaaa ctgtccaagg ctttggcatt aacagccagg tccttgcctg 781 ctgtgcagtc tgatgaaaga cttcagcctc tgctcaatca cctcagtcat tcctacactg 841 gccaagatta cagtacccag ggaaatgttg ggaagatttc tttagatcag attgatttgc 901 tttctaccaa atccttccca ccttgcatgc gtcatttaca taaagccttg cgggaaaatc 961 accatcttcg tcatggaggc cgaatgcagt atggcctatt tctgaagggc attggtttaa 1021 ctttggaaca ggcattgcag ttctggaagc aagaatttat caaaggaaag atggatccag 1081 acaagtttga taaaggttac tcttacaaca tccgtcacag ctttggaaag gaaggcaaga 1141 ggacagacta tacacctttc agttgcctga agattattct gtcCAATCCA CCAAGCCAAG 1201 GGGATTATCA TGGGTGCCCA TTCCGTCACA GTGATCCAGA GCTGCTGAAG CAAAAGTTGC 1261 AGTCATACAA GATCTCTCCT GGAGGGATAA GCCAGATTTT GGATTTAGTA AAGGGGACAC 1321 ATTACCAGGT AGCCTGTCAA AAATACTTTG AGATGATACA CAATGTGGAT GATTGTGGCT 1381 TTTCTTTGAA TCATCCTAAT CAGTTCTTTT GTGAGAGCCA ACGTATTCTA AATGGTGGTA 1441 AAGACATAAA GAAGGAACCT ATCCAACCAG AAACTCCTCA ACCCAAACCA AGTGTCCAGA 1501 AAACCAAGGA TGCATCATCT GCTCTGGCCT CTTTAAATTC CTCTCTGGAA ATGGATATGG 1561 AAGGACTAGA AGATTACTTT AGTGAAGATT CTTACCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1741 ATGACTGGCA TGTACAAGCA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt