Construct: ORF TRCN0000473147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014363.1_s317c1
- Derived from:
- BRDN0000395092
- DNA Barcode:
- TCTATCCCCGTTAGTGATATAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCOA1 (8648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_003743.5 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | XM_005264625.1 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_147233.2 | 99.7% | 99.7% | (many diffs) |
| 4 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | XM_005264626.1 | 99.7% | 99.7% | (many diffs) |
| 5 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_001362950.1 | 96.3% | 96% | (many diffs) |
| 6 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_001362952.1 | 96.3% | 96% | (many diffs) |
| 7 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_001362954.1 | 96.3% | 96% | (many diffs) |
| 8 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_001362955.1 | 96.3% | 96% | (many diffs) |
| 9 | human | 8648 | NCOA1 | nuclear receptor coactivator 1 | NM_147223.3 | 96.3% | 96% | (many diffs) |
| 10 | mouse | 17977 | Ncoa1 | nuclear receptor coactivator 1 | XM_006515005.3 | 89.5% | 92.3% | (many diffs) |
| 11 | mouse | 17977 | Ncoa1 | nuclear receptor coactivator 1 | XM_006515006.3 | 89.5% | 92.3% | (many diffs) |
| 12 | mouse | 17977 | Ncoa1 | nuclear receptor coactivator 1 | XM_006515007.3 | 89.5% | 92.3% | (many diffs) |
| 13 | mouse | 17977 | Ncoa1 | nuclear receptor coactivator 1 | XM_011243840.2 | 89.5% | 92.2% | (many diffs) |
| 14 | mouse | 17977 | Ncoa1 | nuclear receptor coactivator 1 | NM_010881.2 | 86.1% | 88.5% | (many diffs) |
| 15 | mouse | 17977 | Ncoa1 | nuclear receptor coactivator 1 | XM_011243841.2 | 86.1% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4392
- ORF length:
- 4323
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtggcctc ggggacagtt tggccgaccc tgctaaccca gactcacata 121 agaggaaagg atcgccatgt gacacactgg catcaagcac ggaaaagagg cgcagggagc 181 aagaaaataa atatttagaa gaactagctg agttactgtc tgccaacatt agtgacattg 241 acagcttgag tgtaaaacca gacaaatgca agattttgaa gaaaacagtc gatcagatac 301 agctaatgaa gagaatggaa caagagaaat caacaactga tgacgatgta cagaaatcag 361 acatctcatc aagtagtcaa ggagtgatag aaaaggaatc cttgggacct cttcttttgg 421 aggctttgga tggatttttc tttgttgtga actgtgaagg gagaattgta tttgtgtcag 481 agaatgtaac cagctactta ggttacaatc aggaggaatt aatgaatacc agcgtctaca 541 gcatactgca cgtgggggat catgcagaat ttgtgaagaa tctgctacca aaatcactag 601 taaatggagt tccttggcct caagaggcaa cacgacgaaa tagccatacc tttaactgca 661 ggatgctaat tcaccctcca gatgagccag ggaccgagaa ccaagaagct tgccagcgtt 721 atgaagtaat gcagtgtttc actgtgtcac agccaaaatc aattcaagag gatggagaag 781 atttccagtc atgtctgatt tgtattgcac ggcgattacc tcggcctcca gctattacgg 841 gtgtagaatc ctttatgacc aagcaagata ctacaggtaa aatcatctct attgatacta 901 gttccctgag agctgctggc agaactggtt gggaagattt agtgaggaag tgcatttatg 961 cttttttcca acctcagggc agagaaccat cttatgccag acagctgttc caagaagtga 1021 tgactcgtgg cactgcctcc agcccctcct atagattcat attgaatgat gggacaatgc 1081 ttagcgccca caccaagtgt aaactttgct accctcaaag tccagacatg caacctttca 1141 tcatgggaat tcatatcatc gacagggagc acagtgggct ttctcctcaa gatgacacta 1201 attctggaat gtcaattccc cgagtaaatc cctcggtcaa tcctagtatc tctccagctc 1261 atggtgtggc tcgttcatcc acattgccac catccaacag caacatggta tccaccagaa 1321 taaaccgcca gcagagctca gaccttcata gcagcagtca tagtaattct agcaacagcc 1381 aaggaagttt cggatgctca cccggaagtc agattgtagc caatgttgcc ttaaaccaag 1441 gacaggccag ttcacagagc agtaatccct ctttaaacct caataattct cctatggaag 1501 gtacaggaat atccctagca cagttcatgt ctccaaggag acaggttact tctggattgg 1561 caacaaggcc caggatgcca aacaattcct ttcctcctaa tatttcgaca ttaagctctc 1621 ccgttggcat gacaagtagt gcctgtaata ataataaccg atcttattca aacatcccag 1681 taacatcttt acagggtatg aatgaaggac ccaataactc cgttggcttc tctgccagtt 1741 ctccagtcct caggcagatg agctcacaga attcacctag cagattaaat atacaaccag 1801 caaaagctga gtccaaagat aacaaagaga ttgcctcaat tttaaatgaa atgattcaat 1861 ctgacaacag ctctagtgat ggcaaacctc tggattcagg gcttctgcat aacaatgaca 1921 gactttcaga tggagacagt aaatactctc aaaccagtca caaactagtg cagcttttga 1981 caacaactgc cgaacagcag ttacggcatg ctgatataga cacaagctgc aaagatgtcc 2041 tgtcttgcac aggcacttcc aactctgcct ctgctaactc ttcaggaggt tcttgtccct 2101 cttctcatag ctcattgaca gaacggcata aaattctaca ccggctctta caggagggta 2161 gcccctcaga tatcaccact ttgtctgtcg agcctgataa aaaggacagt gcatctactt 2221 ctgtgtcagt gactggacag gtacaaggaa actccagtat aaaactagaa ctggatgctt 2281 caaagaaaaa agaatcaaaa gaccatcagc tcctacgcta tcttttagat aaagatgaga 2341 aagatttaag atcaactcca aacctgagcc tggatgatgt aaaggtgaaa gtggaaaaga 2401 aagaacagat ggatccatgt aatacaaacc caaccccaat gaccaaaccc actcctgagg 2461 aaataaaact ggaggcccag agccagttta cagctgacct tgaccagttt gatcagttac 2521 tgcccacgct ggagaaggca gcacagttgc caggcttatg tgagacagac aggatggatg 2581 gtgcggtcac cagtgtaacc atcaaatcgg agatcctgcc agcttcactt cagtccgcca 2641 ctgccagacc cacttccagg ctaaatagat tacctgagct ggaattggaa gcaattgata 2701 accaatttgg acaaccagga acaggcgatc agattccatg gacaaataat acagtgacag 2761 ctataaatca gagtaaatca gaagaccagt gtattagctc acaattagat gagcttctct 2821 gtccacccac aacagtagaa gggagaaatg atgagaaggc tcttcttgaa cagctggtat 2881 ccttccttag tggcaaagat gaaactgagc tagctgaact agacagagct ctgggaattg 2941 acaaacttgt tcaggggggt ggattagatg tattatcaga gagatttcca ccacaacaag 3001 caacgccacc tttgatcatg gaagaaagac ccaaccttta ttcccagcct tactcttctc 3061 cttctcctac tgccaatctc cctagccctt tccaaggcat ggtcaggcaa aaaccttcac 3121 tggggacgat gcctgttcaa gtaacacctc cccgaggtgc tttttcacct ggcatgggca 3181 tgcagcccag gcaaactcta aacagacctc cggctgcacc taaccagctt cgacttcaac 3241 tacagcagcg attacaggga caacagcagt tgatacacca aaatcggcaa gctatcttaa 3301 accagtttgc agcaactgct cctgttggca tcaatatgag atcaggcatg caacagcaaa 3361 ttacacctca gccacccctg aatgctcaaa tgttggcaca acgtcagcgg gaactgtaca 3421 gtcaacagca ccgacagagg cagctaatac agcagcaaag agccatgctt atgaggcagc 3481 aaagctttgg gaacaacctc cctccctcat ctggactacc agttcaaatg gggaaccccc 3541 gtcttcctca gggtgctcca cagcaattcc cctatccacc aaactatggt acaaatccag 3601 gaaccccacc tgcttctacc agcccgtttt cacaactagc agcaaatcct gaagcatcct 3661 tggccaaccg caacagcatg gtgagcagag gcatgacagg aaacctcggg ggacagtttg 3721 gcactggaat caatcctcag atgcagcaga atgtcttcca gtatccagga gcaggaatgg 3781 ttccccaagg tgaggccaac tttgctccat ctctaagccc tgggagctcc atggtgccga 3841 tgccaatccc tcctcctcag agttctctgc tccagcaaac tccacctgcc TCCGGGTATC 3901 AGTCACCAGA CATGAAGGCC TGGCAGCAAG GAGCGATAGG AAACAACAAT GTGTTCAGTC 3961 AAGCTGTCCA GAACCAGCCC ACGCCTGCAC AGCCAGGAGT ATACAACAAC ATGAGCATCA 4021 CCGTTTCCAT GGCAGGTGGA AATACGAATG TTCAGAACAT GAACCCAATG ATGGCCCAGA 4081 TGCAGATGAG CTCTTTGCAG ATGCCAGGAA TGAACACTGT GTGCCCTGAG CAGATAAATG 4141 ATCCCGCACT GAGACACACA GGCCTCTACT GCAACCAGCT CTCATCCACT GACCTTCTCA 4201 AAACAGAAGC AGATGGAACC CAGCAGGTGC AACAGGTTCA GGTGTTTGCT GACGTCCAGT 4261 GTACAGTGAA TCTGGTAGGC GGGGACCCTT ACCTGAACCA GCCTGGTCCA CTGGGAACTC 4321 AAAAGCCCAC GTCAGGACCA CAGACCCCCC AGGCCCAGCA GAAGAGCCTC CTTCAGCAGC 4381 TACTGACTGA ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 4441 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 4501 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCT ATCCCCGTTA GTGATATAAC 4561 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t