Construct: ORF TRCN0000473153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011035.1_s317c1
- Derived from:
- ccsbBroadEn_07402
- DNA Barcode:
- TGGCCGTGCGGCGTCAATTGTCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RAB28 (9364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_001017979.3 | 99.6% | 22.6% | 24C>T;148delT |
| 2 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_004249.4 | 92.2% | 22.7% | (many diffs) |
| 3 | human | 9364 | RAB28 | RAB28, member RAS oncogene ... | NM_001159601.1 | 87.2% | 24.5% | (many diffs) |
| 4 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_027295.3 | 91.7% | 21.7% | (many diffs) |
| 5 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310589.1 | 85.4% | 21.8% | (many diffs) |
| 6 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | XM_017320585.1 | 85.4% | 21.8% | (many diffs) |
| 7 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | XM_006503651.3 | 84.7% | 12.2% | (many diffs) |
| 8 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310590.1 | 79.9% | 24.8% | (many diffs) |
| 9 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NM_001310592.1 | 59.9% | 11% | (many diffs) |
| 10 | mouse | 100972 | Rab28 | RAB28, member RAS oncogene ... | NR_132325.1 | 31.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 216
- ORF length:
- 150
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggactctgag gaggagagtc aggaccggca actgaaaatc gtcgtgctgg 121 gggacggcgc ctccgggaag acctccttaa cTACGTGTTT TGCTCAAGAA ACTTTTGGGA 181 AACAGTACAA ACAAACTATA GGACTGGATT TCTTTTGAGA AGGATAACAT TGCCAGGAAA 241 CTTGAATGTT ACCCTTCAAA TTTGGGATAT AGGAGGGCAG ACAATAGGAG GCAAAATGTT 301 GGATAAATAT ATCTATGGAG CACAGGGAGT CCTCTTGGTA TATGATATTA CAAATTATCA 361 AAGCTTTGAG AATTTAGAAG ATTGGTATAC TGTGGTGAAG AAAGTGAGCG AGGAGTCAGA 421 AACTCAGCCA CTGGTTGCCT TGGTAGGCAA TAAAATTGAT TTGGAGCATA TGCGAACAAT 481 AAAACCTGAA AAACACTTAC GGTTTTGCCA GGAAAATGGT TTTAGTAGCC ACTTTGTCTC 541 AGCCAAGACA GGAGACTCTG TCTTCCTGTG CTTTCAGAAA GTTGCTGCTG AAATCCTTGG 601 GATCAAATTA AACAAAGCAG AAATAGAACA GTCACAGAGG GTGGTGAAGG CAGATATTGT 661 AAACTACAAC CAGGAACCTA TGTCAAGGAC TGTTAACCCT CCTAGAAGCT CTATGTGTGC 721 AGTTCAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC 781 TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT 841 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGGCCGT GCGGCGTCAA TTGTCCAACG 901 CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt