Construct: ORF TRCN0000473154
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006999.1_s317c1
- Derived from:
- ccsbBroadEn_15642
- DNA Barcode:
- GGAGGCGATCCTGAATTCCGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRADD (8738)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473154
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | NM_001320099.1 | 99.6% | 98.9% | 88C>G;94C>G |
| 2 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | NM_003805.5 | 99.6% | 98.9% | 88C>G;94C>G |
| 3 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | NM_001330126.1 | 61% | 51.2% | (many diffs) |
| 4 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | XM_024449252.1 | 61% | 51.2% | (many diffs) |
| 5 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | XR_001748908.1 | 56.9% | (many diffs) | |
| 6 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | NM_001320101.2 | 55.6% | 48.5% | (many diffs) |
| 7 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | NM_001320100.1 | 53.6% | 52.2% | (many diffs) |
| 8 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | XR_001748909.1 | 53.1% | (many diffs) | |
| 9 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | XM_017020144.1 | 51% | 49.2% | (many diffs) |
| 10 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | XM_017020143.2 | 50.9% | 49.7% | (many diffs) |
| 11 | human | 8738 | CRADD | CASP2 and RIPK1 domain cont... | XM_017020145.1 | 50.7% | 49.7% | (many diffs) |
| 12 | mouse | 12905 | Cradd | CASP2 and RIPK1 domain cont... | NM_001330138.1 | 86.2% | 88.9% | (many diffs) |
| 13 | mouse | 12905 | Cradd | CASP2 and RIPK1 domain cont... | NM_001330141.1 | 86.2% | 88.9% | (many diffs) |
| 14 | mouse | 12905 | Cradd | CASP2 and RIPK1 domain cont... | NM_001330175.1 | 86.2% | 88.9% | (many diffs) |
| 15 | mouse | 12905 | Cradd | CASP2 and RIPK1 domain cont... | NM_009950.3 | 86.2% | 88.9% | (many diffs) |
| 16 | mouse | 12905 | Cradd | CASP2 and RIPK1 domain cont... | XM_006513180.2 | 86.2% | 88.9% | (many diffs) |
| 17 | mouse | 12905 | Cradd | CASP2 and RIPK1 domain cont... | XM_006513181.3 | 86.2% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggccagagac aaacaagtac tccgctcact tcgcctggag ctgggtgcag 121 aggtattggt ggagggactg gttcttcagt acgtctacga ggaaggaatc ttgacggaaa 181 accatattca agaaatcaat gctcaaacca caggcctccg gaaaacaatg ctcctgctgg 241 atatcctacc TTCCAGGGGC CCTAAAGCAT TTGATACATT CCTAGATTCC CTACAGGAGT 301 TTCCCTGGGT CAGGGAGAAG CTGAAGAAGG CAAGGGAAGA GGCCATGACC GACCTGCCTG 361 CAGGTGACAG ATTGACTGGG ATCCCCTCGC ACATCCTCAA CAGCTCCCCA TCAGACCGGC 421 AGATTAACCA GCTGGCCCAG AGGCTGGGCC CTGAGTGGGA GCCCATGGTG CTGTCTCTGG 481 GACTGTCCCA GACGGATATC TACCGCTGTA AGGCCAACCA CCCCCACAAC GTGCAGTCGC 541 AGGTGGTGGA GGCCTTCATC CGTTGGCGGC AGCGCTTCGG GAAGCAGGCC ACCTTCCAGA 601 GCCTGCACAA CGGGCTGCGG GCTGTGGAGG TGGACCCCTC GCTGCTCCTG CACATGTTGG 661 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAGG AGGCGATCCT GAATTCCGGT TCACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt