Construct: ORF TRCN0000473176

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004438.1_s317c1
Derived from:
ccsbBroadEn_13853
DNA Barcode:
TATGTAAATCCTCTGCTGACGAAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EPB41L1 (2036)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473176
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2036 EPB41L1 erythrocyte membrane protei... NM_001258329.1 99.9% .5% 12delG
2 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027716.1 99.9% .5% 12delG
3 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027717.2 99.9% .5% 12delG
4 human 2036 EPB41L1 erythrocyte membrane protei... NM_012156.2 99.8% .5% 12delG;2182_2184delCAG
5 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528681.1 98.5% .5% 12delG;1447_1448ins36
6 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528682.1 98.4% .5% 12delG;1298_1299insAGC;1444_1445ins36
7 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027715.1 98.3% .5% (many diffs)
8 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528683.1 96.8% .5% 12delG;2183_2184ins81
9 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027712.2 94.2% .4% (many diffs)
10 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027713.2 92.9% .4% (many diffs)
11 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027714.2 89.9% .4% (many diffs)
12 human 2036 EPB41L1 erythrocyte membrane protei... NM_001258331.2 88.5% .3% 0_1ins185;1261_1262ins36;1961_1962ins81
13 human 2036 EPB41L1 erythrocyte membrane protei... NM_177996.2 88.5% .3% 0_1ins185;1261_1262ins36;1961_1962ins81
14 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528685.1 84.2% .6% 12delG;1664_1665ins411;1771_1773delCAG
15 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027720.1 79.9% .7% (many diffs)
16 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027718.2 78.8% .5% (many diffs)
17 human 2036 EPB41L1 erythrocyte membrane protei... NM_001258330.1 77.5% .5% (many diffs)
18 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027719.2 77.5% .5% (many diffs)
19 human 2036 EPB41L1 erythrocyte membrane protei... XM_024451854.1 74.3% .6% 0_1ins185;1478_1479ins411;1586_1587ins81
20 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528671.1 56.3% .3% 12delG;1668_3704del;4219_4221delCAG
21 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528673.1 56.3% .3% 12delG;1668_3704del;4219_4221delCAG
22 human 2036 EPB41L1 erythrocyte membrane protei... XM_024451851.1 53.8% .1% (many diffs)
23 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528667.1 53.5% .2% (many diffs)
24 human 2036 EPB41L1 erythrocyte membrane protei... XM_011528677.1 52.4% .2% 0_1ins185;1482_3518del;4033_4035delCAG
25 human 2036 EPB41L1 erythrocyte membrane protei... XM_017027711.1 52.4% .2% 0_1ins185;1482_3518del;4033_4035delCAG
26 human 2036 EPB41L1 erythrocyte membrane protei... XM_024451852.1 52.4% .2% 0_1ins185;1482_3518del;4033_4035delCAG
27 human 2036 EPB41L1 erythrocyte membrane protei... XM_024451853.1 52.4% .2% 0_1ins185;1482_3518del;4033_4035delCAG
28 mouse 13821 Epb41l1 erythrocyte membrane protei... NM_001006664.3 90.8% .5% (many diffs)
29 mouse 13821 Epb41l1 erythrocyte membrane protei... NM_001291120.1 90.8% .5% (many diffs)
30 mouse 13821 Epb41l1 erythrocyte membrane protei... NM_013510.4 90.8% .5% (many diffs)
31 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_017315393.1 90.7% .5% (many diffs)
32 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_017315394.1 90% .5% (many diffs)
33 mouse 13821 Epb41l1 erythrocyte membrane protei... NM_001291121.1 89.5% .5% (many diffs)
34 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239281.1 76.8% .6% (many diffs)
35 mouse 13821 Epb41l1 erythrocyte membrane protei... NM_001291122.1 75.6% .6% (many diffs)
36 mouse 13821 Epb41l1 erythrocyte membrane protei... NM_001291123.1 75.4% .6% (many diffs)
37 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_006498711.3 50.2% .3% (many diffs)
38 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_006498713.3 50.2% .3% (many diffs)
39 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_006498714.3 50.2% .3% (many diffs)
40 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239280.2 50.2% .3% (many diffs)
41 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_017315383.1 50.2% .3% (many diffs)
42 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239275.2 50.1% .3% (many diffs)
43 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_006498708.3 49.8% .3% (many diffs)
44 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239276.1 49.7% .3% (many diffs)
45 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239277.1 49.5% .3% (many diffs)
46 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239278.1 49% .3% (many diffs)
47 mouse 13821 Epb41l1 erythrocyte membrane protei... XM_011239279.1 46.7% .3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
96
ORF length:
30
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac aacagaacag gccccgactc tgaggtgaag aaagctcagg aggaggcccc
121gcagcagccc gaggctgctg ccgctgtgac cacccctgtg acccctgcag gccacggcca
181cccagaggcc aactccaatg agaagcatcc atcccagcag gacacgcggc ctgctgaaca
241gagcctagac atggaggaga aggactacag tgaggccgat ggcctttcgg agaggaccac
301gcccagcaag gcccagaaat cgccccagaa gattgccaag aaatacaaga gtgccatctg
361ccgggtcact ctgcttgatg cctcggagta tgagtgtgag gtggagaaac atggccgggg
421ccaggtgctg tttgacctgg tctgtgaaca cctcaacctc ctagagaagg actacttcgg
481cctgaccttc tgtgatgctg acagccagaa gaactggctg gacccctcca aggagatcaa
541gaagcagatc cggagtagcc cctggaattt tgccttcaca gtcaagttct acccgcctga
601tcctgcccag ctgacagaag acatcacaag atactacctg tgcctgcagc tgcgggcaga
661catcatcacg ggccggctgc catgctcctt tgtcacgcat gccctactgg gctcctacgc
721tgtgcaggct gagctgggtg actatgatgc tgaggagcat gtgggcaact atgtcagcga
781gctccgcttc gcccctaacc agacccggga gctggaggag aggatcatgg agctgcataa
841gacatatagg gggatgaccc cgggagaagc agaaatccac ttcttagaga atgccaagaa
901gctttccatg tacggagtag acctgcacca tgccaaggac tctgagggca tcgacatcat
961gttaggcgtt tgtgccaatg gcctgctcat ctaccgggac cggctgagaa tcaaccgctt
1021tgcctggccc aagatcctca agatctccta caagaggagt aacttctata tcaagatccg
1081gcctggggag tatgagcaat ttgagagcac aattggcttt aagctcccaa accaccggtc
1141agccaagaga ctgtggaagg tctgcatcga gcatcataca ttcttccggc tggtgtcccc
1201tgagccccca cccaagggct tcctggtgat gggctccaag ttccggtaca gtgggaggac
1261ccaggcacag actcgccagg ccagcgccct cattgaccgg cctgcaccct tctttgagcg
1321ttcttccagc aaacggtaca ccatgtcccg cagccttgat ggagcagagt tctcccgccc
1381agcctcggtc agcgagaacc atgatgcagg gcctgacggt gacaagcggg atgaggatgg
1441cgagtctggg gggcaacggt cagaggctga ggagggagag gtcaggactc caaccaagat
1501caaggagcta aagccggagc aggaaaccac gccgagacac aagcaggagt tcttagacaa
1561gccagaagat gtcttgctga agcaccaggc cagcatcaat gagctcaaaa ggaccctgaa
1621ggagcccaac agcaaactca tccaccggga tcgagactgg gaacgggagc gcaggctgcc
1681ctcctccccc gcctccccct cccccaaggg cacccctgag aaagccaatg agagagcagg
1741gctgagggag ggctccgagg agaaagtcaa accaccacgt ccccgggccc cagagagtga
1801cacaggcgat gaggaccagg accaggagag ggacacggtg ttcctgaagg acaaccacct
1861ggccattgag cgcaagtgct ccagcatcac ggtcagctct acgtctagcc tggaggctga
1921ggtggacttc acggtcattg gtgactacca tggcagcgcc ttcgaagact tctcccgcag
1981cctgcctgag ctcgaccggg acaaaagcga ctcggacact gagggcctgc tgttctcccg
2041ggatctcaac aagggggccc ccagccagga tgatgagtct gggggcattg aggacagccc
2101ggatcgaggg gcctgctcca ccccggatat gccccagttt gagcccgtga aaacagaaac
2161catgactgtc agcagtctgg ccattagaaa gaagattgag ccggaggccg tactgcagac
2221cagagtctcc gctatggata acacccaggt tgatgggagt gcctcagtgg ggagggagtt
2281catagcaacc actccctcca tcaccacgga gaccatatcg accaccatgg agaacagtct
2341caagtccggg aagggggcag ctgccatgat cccaggccca cagacggtgg ccacggaaat
2401ccgttctctt tctccgatca tcgggaaaga tgtcctcacc agcacctacg gcgccactgc
2461ggaaaccctc tcaacctcca ccaccaccca tgtcaccaaa actgtgaaag gagggttttc
2521tgagacaagg atcgagaagc gaatcatcat tactggggat gaagatgtcg atcaagacca
2581ggccctggct ttggccatca aggaggccaa actgcagcat cctgatatgc tggtaaccaa
2641agctgtcgta tacagagaaa cagacccatc cccagaggag agGGACAAGA AGCCACAGGA
2701ATCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT
2761CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC
2821TTGGCTTTAT ATATCTTGTG GAAAGGACGA TATGTAAATC CTCTGCTGAC GAAAACGCGT
2881TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)