Construct: ORF TRCN0000473183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009384.1_s317c1
- Derived from:
- ccsbBroadEn_02181
- DNA Barcode:
- GCGCATACCTTTCGCATTTTCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LITAF (9516)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9516 | LITAF | lipopolysaccharide induced ... | NM_001136472.1 | 100% | 100% | |
| 2 | human | 9516 | LITAF | lipopolysaccharide induced ... | NM_004862.3 | 100% | 100% | |
| 3 | human | 9516 | LITAF | lipopolysaccharide induced ... | XM_006720982.3 | 100% | 100% | |
| 4 | human | 9516 | LITAF | lipopolysaccharide induced ... | XM_006720983.4 | 100% | 100% | |
| 5 | human | 9516 | LITAF | lipopolysaccharide induced ... | XM_006720984.4 | 100% | 100% | |
| 6 | human | 9516 | LITAF | lipopolysaccharide induced ... | XM_006720985.3 | 100% | 100% | |
| 7 | human | 9516 | LITAF | lipopolysaccharide induced ... | XM_017023896.1 | 100% | 100% | |
| 8 | human | 9516 | LITAF | lipopolysaccharide induced ... | XM_011522754.3 | 84.2% | 84.2% | 1_90del |
| 9 | human | 9516 | LITAF | lipopolysaccharide induced ... | NM_001136473.1 | 84.1% | 74.7% | (many diffs) |
| 10 | human | 9516 | LITAF | lipopolysaccharide induced ... | NR_024320.1 | 18.9% | 1_134del;618_2543del | |
| 11 | mouse | 56722 | Litaf | LPS-induced TN factor | NM_019980.2 | 80.6% | 85.7% | (many diffs) |
| 12 | mouse | 56722 | Litaf | LPS-induced TN factor | XM_006522427.1 | 80.6% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 552
- ORF length:
- 483
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggttcca ggaccttacc aggcggccac tgggccttcc tcagcaccat 121 ccgcacctcc atcctatgaa gagacagtgg ctgttaacag ttattacccc acacctccag 181 ctcccatgcc tgggccaact acggggcttg tgacggggcc tgatgggaag ggcatgaatc 241 ctccttcgta ttatacccag ccagcgccca tccccaataa caatccaatt accgtgcaga 301 cggtcTACGT GCAGCACCCC ATCACCTTTT TGGACCGCCC TATCCAAATG TGTTGTCCTT 361 CCTGCAACAA GATGATCGTG AGTCAGCTGT CCTATAACGC CGGTGCTCTG ACCTGGCTGT 421 CCTGCGGGAG CCTGTGCCTG CTGGGGTGCA TAGCGGGCTG CTGCTTCATC CCCTTCTGCG 481 TGGATGCCCT GCAGGACGTG GACCATTACT GTCCCAACTG CAGAGCTCTC CTGGGCACCT 541 ACAAGCGTTT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCG CATACCTTTC GCATTTTCCA 721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t