Construct: ORF TRCN0000473208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004034.1_s317c1
- Derived from:
- ccsbBroadEn_08525
- DNA Barcode:
- ACGCAAGCATAACAGAGCTCCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STRBP (55342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55342 | STRBP | spermatid perinuclear RNA b... | NM_018387.5 | 99.9% | 100% | 960C>T |
| 2 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_017014896.1 | 99.9% | 100% | 960C>T |
| 3 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_024447602.1 | 99.9% | 100% | 960C>T |
| 4 | human | 55342 | STRBP | spermatid perinuclear RNA b... | NM_001171137.2 | 97.8% | 97.9% | 0_1ins42;918C>T |
| 5 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_017014897.1 | 97.7% | 97.7% | 960C>T;1896_1897ins45 |
| 6 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_017014890.1 | 97% | 96.4% | (many diffs) |
| 7 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_017014891.2 | 97% | 96.4% | (many diffs) |
| 8 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_017014892.1 | 97% | 96.4% | (many diffs) |
| 9 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_017014898.1 | 96.3% | 96.4% | 960C>T;1944_1945ins72 |
| 10 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_024447603.1 | 96.3% | 96.4% | 960C>T;1944_1945ins72 |
| 11 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XR_001746349.1 | 75.7% | (many diffs) | |
| 12 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XR_001746348.1 | 74.8% | (many diffs) | |
| 13 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_024447605.1 | 72.8% | 72.9% | 0_1ins546;414C>T |
| 14 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XM_024447604.1 | 70.3% | 69.5% | (many diffs) |
| 15 | human | 55342 | STRBP | spermatid perinuclear RNA b... | NR_033234.2 | 31.4% | (many diffs) | |
| 16 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XR_001746347.1 | 22.3% | (many diffs) | |
| 17 | human | 55342 | STRBP | spermatid perinuclear RNA b... | XR_001746346.1 | 22% | (many diffs) | |
| 18 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | NM_009261.3 | 93.9% | 98.9% | (many diffs) |
| 19 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_011239046.2 | 93.9% | 98.9% | (many diffs) |
| 20 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_017316820.1 | 93.9% | 98.9% | (many diffs) |
| 21 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_017316821.1 | 93.9% | 98.9% | (many diffs) |
| 22 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_017316822.1 | 93.9% | 98.9% | (many diffs) |
| 23 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_017316823.1 | 93.9% | 98.9% | (many diffs) |
| 24 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_011239047.2 | 90.5% | 95.3% | (many diffs) |
| 25 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_011239043.2 | 90.3% | 91.9% | (many diffs) |
| 26 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_011239044.2 | 90.3% | 91.9% | (many diffs) |
| 27 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_011239045.2 | 90.3% | 91.9% | (many diffs) |
| 28 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XM_017316819.1 | 90.3% | 91.9% | (many diffs) |
| 29 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XR_866011.2 | 45.2% | (many diffs) | |
| 30 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XR_001781603.1 | 43.4% | (many diffs) | |
| 31 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XR_866010.2 | 42.3% | (many diffs) | |
| 32 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XR_866009.2 | 41.8% | (many diffs) | |
| 33 | mouse | 20744 | Strbp | spermatid perinuclear RNA b... | XR_001781604.1 | 24% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2082
- ORF length:
- 2016
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag atctattcga tcttttgcta atgatgatcg ccatgttatg gtgaaacatt 121 caacaatcta tccatctccg gaggaacttg aagctgttca gaatatggta tctactgttg 181 aatgtgctct taaacatgtc tcagattggt tggatgaaac aaataaaggc acaaaaacag 241 agggtgagac agaagtgaag aaagatgagg ccggagaaaa ctattccaag gatcaaggtg 301 gtcggacatt gtgtggtgta atgaggattg gcctggttgc aaaaggcttg ctgattaaag 361 atgatatgga cttggagctg gttttaatgt gcaaagacaa acccacagag accctgttaa 421 atacagtcaa agataatctt cctattcaga ttcagaaact cacagaagag aaatatcaag 481 tggaacaatg tgtaaatgag gcatctatta taattcggaa tacaaaagag cccacgctaa 541 ctttgaaggt gatacttacc tcacctctaa ttagggacga attggagaag aaggatggag 601 aaaatgtttc gatgaaagat cctccggact tattggacag gcagaaatgc ctgaacgcct 661 tggcgtctct tcgacatgcc aaatggtttc aggcaagggc aaatggatta aaatcatgtg 721 taattgtcct ccgcattctg cgtgatttgt gcaacagagt ccccacatgg gcaccattga 781 aaggatggcc actagaactt atatgtgaaa agtctatagg tacttgtaat agacctttgg 841 gcgctgggga ggccttgaga cgagtaatgg agtgtttggc atctggaata ctacttcctg 901 ggggtcctgg tcttcatgat ccttgtgagc gagacccaac agatgctctg agctatatga 961 ccatccagca aaaagaagat attacccaca gtgcacagca tgcactcaga ctatcagcct 1021 ttggtcagat ttacaaagtg ctggagatgg acccccttcc atctagtaag ccttttcaga 1081 agtattcctg gtcagttact gataaagaag gtgctgggtc ttcagctcta aagaggccat 1141 ttgaagatgg attaggggat gataaagacc ccaacaagaa gatgaaacga aacttaagga 1201 aaattctgga tagtaaagca atagacctta tgaatgcact aatgaggcta aatcagatca 1261 ggcctgggct tcagtataag ctcctatctc agtctggccc cgttcatgcc ccagtcttca 1321 caatgtctgt agatgtggat ggcacaacat atgaagcctc aggaccatcc aagaaaacag 1381 caaaacttca cgtagcggtg aaggtattgc aggcaatggg atatccaaca ggctttgatg 1441 cagatattga atgtatgagt tccgatgaaa aatcagataa tgaaagtaaa aatgaaacag 1501 tgtcttcaaa ctcaagcaat aatactggaa attctacaac tgaaacctcc agtaccttag 1561 aggtaagaac tcagggccct atcctcacag caagtggcaa aaaccctgta atggagctca 1621 atgaaaaaag aagaggtctc aagtatgaac tcatctcaga gactggtgga agccatgaca 1681 AGCGCTTTGT AATGGAGGTA GAAGTAGATG GACAGAAATT CAGAGGCGCA GGTCCAAATA 1741 AGAAAGTGGC AAAGGCGAGT GCAGCTTTAG CTGCCTTGGA GAAACTGTTT TCTGGACCCA 1801 ATGCGGCAAA TAATAAGAAA AAGAAGATTA TCCCTCAGGC AAAGGGCGTT GTGAATACAG 1861 CTGTGTCTGC AGCAGTCCAA GCTGTTCGGG GCAGAGGAAG AGGAACTCTA ACAAGGGGAG 1921 CTTTTGTTGG GGCGACAGCT GCTCCTGGCT ACATAGCTCC AGGCTATGGA ACACCATATG 1981 GTTACAGCAC AGCTGCCCCT GCCTATGGTT TACCCAAGAG AATGGTTCTG TTACCCGTTA 2041 TGAAATTTCC AACATATCCT GTTCCCCACT ACTCATTCTT TTACCCAACT TTCTTGTACA 2101 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2161 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2221 AGGACGAACG CAAGCATAAC AGAGCTCCCT TACGCGTTAA GTCgacaatc aacctctgga 2281 ttacaaaatt tgtgaaagat t