Construct: ORF TRCN0000473219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012078.1_s317c1
- Derived from:
- ccsbBroadEn_12231
- DNA Barcode:
- CTCACAAGAGGAACCAGCTTATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKN2AIP (55602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473219
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55602 | CDKN2AIP | CDKN2A interacting protein | NM_017632.4 | 99.8% | 99.8% | 722_724delCTG |
| 2 | human | 55602 | CDKN2AIP | CDKN2A interacting protein | NM_001317343.2 | 21.7% | 21.5% | 372_373insGTGAAGAA;376G>A;378_379ins1351 |
| 3 | mouse | 70925 | Cdkn2aip | CDKN2A interacting protein | NM_172407.3 | 82.8% | 84.4% | (many diffs) |
| 4 | mouse | 70925 | Cdkn2aip | CDKN2A interacting protein | XM_006509489.2 | 18.4% | 18.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcaggaggtg tcggagtacc tgagccagaa cccgcgggtg gcagcctggg 121 tggaggcgct gcgctgcgac ggcgagactg acaaacactg gcgccaccgc cgggattttt 181 tgcttcgcaa cgccggggac ctggcccccg ctggcggcgc tgcctccgct agcacggatg 241 aagctgccga cgccgagagc gggacccgaa accggcagct gcagcagctc atctcctttt 301 ccatggcctg ggcgaaccac gtcttcctcg ggtgccgata ccctcaaaaa gttatggata 361 aaatacttag tatggctgaa ggcatcaaag tgacagatgc tccaacctat acaacaagag 421 atgaactggt tgccaaggtg aagaaaagag ggatatcgag tagcaatgaa ggggtagaag 481 agccatccaa aaaacgagtt atagaaggaa aaaacagttc tgcagttgag caagatcacg 541 caaaaacctc tgccaagaca gaacgtgcat cagctcagca ggaaaacagt tcaacgtgta 601 tagggtcggc catcaaatca gagagtggga actcagctcg gagctctggc atctccagtc 661 agaatagctc tacaagtgat ggagatcgat ctgtttccag ccaaagcagc agcagcgttt 721 cctctcaggt aacaacggca ggatctggga aagcttctga agcagaagct ccagataaac 781 acggttcatc atttgtttcc ttgctgaaat ccagtgtgaa tagtcacatg acccaatcca 841 ctgattctag acaacaaagt ggatcaccta aaaagagtgc tttggaaggc tcttcagcct 901 cagcttctca aagcagctca gagatcgagg tgcccttgtt gggctcctca ggaagctcag 961 aggtagaatt gccactattg tcttccaaac ctagttcaga gacagcttca agtgggttaa 1021 cttccaaaac tagttcagag gcaagtgttt catcatcagt tgctaaaaac agttcctcat 1081 caggcacatc cttactgact cccaagagca gctcttcaac aaatacatcg ctgctaactt 1141 ccaagagcac ttcccaggta gctgcatcac tactagcttc caagagcagc tcccagacca 1201 gtggatctct ggtttccaaa agcacttcct tagcaagtgt gtcccagttg gcttctaaga 1261 gtagttctca gactagcacc tcacagttgC CTTCTAAAAG TACTTCACAG TCAAGTGAGA 1321 GTTCTGTCAA ATTCTCTTGC AAGTTAACCA ATGAAGATGT GAAACAGAAG CAACCTTTTT 1381 TCAATAGACT ATATAAAACG GTGGCATGGA AGTTGGTAGC TGTTGGTGGC TTTAGTCCCA 1441 ATGTGAATCA TGGAGAGCTC CTAAATGCAG CTATTGAGGC TCTGAAAGCA ACACTGGATG 1501 TATTTTTTGT CCCACTAAAA GAATTGGCAG ATCTGCCTCA AAATAAGAGC TCTCAAGAAA 1561 GTATTGTTTG TGAATTGAGG TGCAAGTCTG TGTATTTGGG CACTGGCTGT GGAAAAAGCA 1621 AAGAAAATGC AAAAGCAGTT GCATCAAGAG AAGCATTGAA GTTATTTCTC AAGAAAAAGG 1681 TGGTGGTAAA AATATGTAAA AGGAAATACA GAGGCAGTGA AATAGAAGAT CTAGTACTCC 1741 TTGATGAAGA ATCGAGGCCT GTAAACTTAC CTCCAGCACT AAAACATCCT CAAGAATTAC 1801 TATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGACT CACAAGAGGA ACCAGCTTAT CCACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt