Construct: ORF TRCN0000473224
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015587.1_s317c1
- Derived from:
- ccsbBroadEn_06602
- DNA Barcode:
- TACAGGCAATACACCTGTCCTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTM1 (4534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473224
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4534 | MTM1 | myotubularin 1 | NM_000252.2 | 99.9% | 99.8% | 1228G>A |
| 2 | human | 4534 | MTM1 | myotubularin 1 | XM_005274687.2 | 99.9% | 99.8% | 1228G>A |
| 3 | human | 4534 | MTM1 | myotubularin 1 | XM_011531173.2 | 99.9% | 99.8% | 1228G>A |
| 4 | human | 4534 | MTM1 | myotubularin 1 | XM_017029549.1 | 99.7% | 99.6% | 1228G>A;1644_1645insCAG |
| 5 | human | 4534 | MTM1 | myotubularin 1 | XM_011531171.1 | 97.5% | 97.4% | 231_275del;1273G>A |
| 6 | human | 4534 | MTM1 | myotubularin 1 | XM_011531172.1 | 97.5% | 97.4% | 231_275del;1273G>A |
| 7 | human | 4534 | MTM1 | myotubularin 1 | XM_017029548.1 | 97.5% | 97.4% | 231_275del;1273G>A |
| 8 | human | 4534 | MTM1 | myotubularin 1 | XM_017029547.1 | 97.3% | 97.2% | 231_275del;1273G>A;1689_1690insCAG |
| 9 | human | 4534 | MTM1 | myotubularin 1 | XM_017029550.1 | 93.8% | 93.6% | 231_232ins111;1117G>A |
| 10 | human | 4534 | MTM1 | myotubularin 1 | XM_017029551.2 | 58.8% | 58.7% | 0_1ins744;484G>A |
| 11 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164190.1 | 88.7% | 91.8% | (many diffs) |
| 12 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164191.1 | 88.7% | 91.8% | (many diffs) |
| 13 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_019926.3 | 88.7% | 91.8% | (many diffs) |
| 14 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164192.1 | 84.1% | 86.8% | (many diffs) |
| 15 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | XM_006527890.1 | 80.1% | 82.5% | (many diffs) |
| 16 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | XM_006527891.1 | 74.6% | 78.1% | (many diffs) |
| 17 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | XM_017318416.1 | 63.8% | 65.9% | (many diffs) |
| 18 | mouse | 17772 | Mtm1 | X-linked myotubular myopath... | NM_001164193.1 | 23.4% | 21.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1875
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttctgcatca acttctaaat ataattcaca ctccttggag aatgagtcta 121 ttaagaggac gtctcgagat ggagtcaatc gagatctcac tgaggctgtt cctcgacttc 181 caggagaaac actaatcact gacaaagaag ttatttacat atgtcctttc aatggcccca 241 ttaagggaag agtttacatc acaaattatc gtctttattt aagaagtttg gaaacggatt 301 cttctctaat acttgatgtt cctctgggtg tgatctcgag aattgaaaaa atgggaggcg 361 cgacaagtag aggagaaaat tcctatggtc tagatattac ttgtaaagac atgagaaacc 421 tgaggttcgc tttgaaacag gaaggccaca gcagaagaga tatgtttgag atcctcacga 481 gatacgcgtt tcccctggct cacagtctgc cattatttgc atttttaaat gaagaaaagt 541 ttaacgtgga tggatggaca gtttacaatc cagtggaaga atacaggagg cagggcttgc 601 ccaatcacca ttggagaata acttttatta ataagtgcta tgagctctgt gacacttacc 661 ctgctctttt ggtggttccg tatcgtgcct cagatgatga cctccggaga gttgcaactt 721 ttaggtcccg aaatcgaatt ccagtgctgt catggattca tccagaaaat aagacggtca 781 ttgtgcgttg cagtcagcct cttgtcggta tgagtgggaa acgaaataaa gatgatgaga 841 aatatctcga tgttatcagg gagactaata aacaaatttc taaactcacc atttatgatg 901 caagacccag cgtaaatgca gtggccaaca aggcaacagg aggaggatat gaaagtgatg 961 atgcatatca taacgccgaa cttttcttct tagacattca taatattcat gttatgcggg 1021 aatctttaaa aaaagtgaag gacattgttt atcctaatgt agaagaatct cattggttgt 1081 ccagtttgga gtctactcat tggttagaac atatcaagct cgttttgaca ggagccattc 1141 aagtagcaga caaagtttct tcagggaaga gttcagtgct tgtgcattgc agtgacggat 1201 gggacaggac tgctcagctg acatccttgg ccatgctgat gttggatagc ttctatagga 1261 gcattgaagg gttcgaaata ctggtacaaa aaaaatggat aagttttgga cataaatttg 1321 catctcgaat aggtcatggt gataaaaacc acaccGATGC TGACCGTTCT CCTATTTTTC 1381 TCCAGTTTAT TGATTGTGTG TGGCAAATGT CAAAACAGTT CCCTACAGCT TTTGAATTCA 1441 ATGAACAATT TTTGATTATA ATTTTGGATC ATCTGTATAG TTGCCGATTT GGTACTTTCT 1501 TATTCAACTG TGAATCTGCT CGAGAAAGAC AGAAGGTTAC AGAAAGGACT GTTTCTTTAT 1561 GGTCACTGAT AAACAGTAAT AAAGAAAAAT TCAAAAACCC CTTCTATACT AAAGAAATCA 1621 ATCGAGTTTT ATATCCAGTT GCCAGTATGC GTCACTTGGA ACTCTGGGTG AATTACTACA 1681 TTAGATGGAA CCCCAGGATC AAGCAACAAC AGCCGAATCC AGTGGAGCAG CGTTACATGG 1741 AGCTCTTAGC CTTACGCGAC GAATACATAA AGCGGCTTGA GGAACTGCAG CTCGCCAACT 1801 CTGCCAAGCT TTCTGATCCC CCAACTTCAC CTTCCAGTCC TTCGCAAATG ATGCCCCATG 1861 TGCAAACTCA CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1921 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1981 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACAGGCAAT ACACCTGTCC 2041 TGGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt