Construct: ORF TRCN0000473228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008440.1_s317c1
- Derived from:
- ccsbBroadEn_06483
- DNA Barcode:
- ATCAGGACTCTTCCTTCGAACTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNK1 (3775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3775 | KCNK1 | potassium two pore domain c... | NM_002245.4 | 99.9% | 100% | 900C>A |
| 2 | human | 3775 | KCNK1 | potassium two pore domain c... | XM_011544184.2 | 71.8% | 64.5% | (many diffs) |
| 3 | mouse | 16525 | Kcnk1 | potassium channel, subfamil... | NM_008430.2 | 88.7% | 94.9% | (many diffs) |
| 4 | mouse | 16525 | Kcnk1 | potassium channel, subfamil... | XM_006530716.2 | 85.7% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1074
- ORF length:
- 1008
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcagtccctg gccggcagct cgtgcgtgcg cctggtggag cggcaccgct 121 cggcctggtg cttcggcttc ctggtgctgg gctacttgct ctacctggtc ttcggcgcag 181 tggtcttctc ctcggtggag ctgccctatg aggacctgct gcgccaggag ctgcgcaagc 241 tgaagcgacg cttcttggag gagcacgagt gcctgtctga gcagcagctg gagcagttcc 301 tgggccgggt gctggaggcc agcaactacg gcgtgtcggt gctcagcaac gcctcgggca 361 actggaactg ggacttcacc tccgcgctct tcttcgccag caccgtgctc tccaccacag 421 gttatggcca caccgtgccc ttgtcagatg gaggtaaggc cttctgcatc atctactccg 481 tcattggcat tcccttcacc ctcctgttcc tgacggctgt ggtccagcgc atcaccgtgc 541 acgtcacccg caggccggtc ctctacttcc acatccgctg gggcttctcc aagcaggtgg 601 tggccatcgt ccatgccgtg ctccttgggt ttgtcactgt gtcctgcttc ttcttcatcc 661 cggccgctgt cttctcagtc ctggaggatg actggaactt cctGGAATCC TTTTATTTTT 721 GTTTTATTTC CCTGAGCACC ATTGGCCTGG GGGATTATGT GCCTGGGGAA GGCTACAATC 781 AAAAATTCAG AGAGCTCTAT AAGATTGGGA TCACGTGTTA CCTGCTACTT GGCCTTATTG 841 CCATGTTGGT AGTTCTGGAA ACCTTCTGTG AACTCCATGA GCTGAAAAAA TTCAGAAAAA 901 TGTTCTATGT GAAGAAGGAC AAGGACGAGG ATCAGGTGCA CATCATAGAG CATGACCAAC 961 TGTCATTCTC CTCGATCACA GACCAGGCAG CTGGCATGAA AGAGGACCAG AAGCAAAATG 1021 AGCCTTTTGT GGCCACCCAG TCATCTGCCT GCGTGGATGG CCCTGCAAAC CATTGCCCAA 1081 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1141 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1201 TATCTTGTGG AAAGGACGAA TCAGGACTCT TCCTTCGAAC TCCACGCGTT AAGTCgacaa 1261 tcaacctctg gattacaaaa tttgtgaaag att