Construct: ORF TRCN0000473265
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015211.1_s317c1
- Derived from:
- BRDN0000395119
- DNA Barcode:
- CTGACTACCTTCCTCTTTTGTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTAF1 (9044)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473265
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | NM_003972.3 | 99.9% | 99.9% | 3625T>A;3627C>T |
| 2 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_011540326.2 | 97.9% | 97.9% | 1402_1403ins111;3514T>A;3516C>T |
| 3 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_017016877.1 | 97.3% | 97.2% | 251_252ins147;3478T>A;3480C>T |
| 4 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_011540327.2 | 93% | 92.8% | (many diffs) |
| 5 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_011540328.2 | 91.5% | 91.5% | 0_1ins468;3157T>A;3159C>T |
| 6 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_017016878.2 | 88.9% | 88.9% | 0_1ins609;3016T>A;3018C>T |
| 7 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_017016879.2 | 82.1% | 82% | 0_1ins990;2635T>A;2637C>T |
| 8 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_017016880.2 | 82.1% | 82% | 0_1ins990;2635T>A;2637C>T |
| 9 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_017016881.2 | 82.1% | 82% | 0_1ins990;2635T>A;2637C>T |
| 10 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XR_246113.3 | 69.2% | (many diffs) | |
| 11 | human | 9044 | BTAF1 | B-TFIID TATA-box binding pr... | XM_011540329.3 | 59.7% | 59.6% | (many diffs) |
| 12 | mouse | 107182 | Btaf1 | B-TFIID TATA-box binding pr... | NM_001080706.1 | 91.3% | 96.2% | (many diffs) |
| 13 | mouse | 107182 | Btaf1 | B-TFIID TATA-box binding pr... | XM_006526561.3 | 81.2% | 85.6% | (many diffs) |
| 14 | mouse | 107182 | Btaf1 | B-TFIID TATA-box binding pr... | XM_017318039.1 | 52.6% | 55.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5616
- ORF length:
- 5547
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggtctcc aggctagatc gcctttttat tttactggat actggcacta 121 ctcctgttac aagaaaagct gctgcacagc aacttggaga agtggtgaag cttcatcccc 181 atgaactaaa taatctcctg tctaaagtgt tgatatattt aaggagtgca aattgggata 241 cccggattgc agcaggacaa gctgttgaag ctatagtgaa aaatgtacct gagtggaatc 301 cagtgccgag aaccagacaa gaacctactt ccgaaagttc tatggaagat tcacctacta 361 cagagcgatt gaattttgac agatttgata tatgtagatt gttacaacat ggtgcatcac 421 tcctgggatc tgctggtgcc gaatttgaag tccaagatga aaaatcaggt gaagtggatc 481 ctaaagagag gatagcacgc caacgaaaat tattacagaa gaaacttggc cttaatatgg 541 gagaagcaat tggaatgagt actgaagaac ttttcaatga tgaggatttg gattataccc 601 caacttcagc atcctttgtt aacaaacaac ctactcttca ggcagctgaa ttgattgact 661 cagagtttcg agcaggaatg agcaatagac aaaagaacaa agctaaaaga atggccaagt 721 tatttgcaaa acagagatcc agggatgcag tggaaactaa tgagaagagc aatgatagca 781 ctgatgggga gccagaagaa aagagacgga aaatagcaaa tgttgttatt aatcagtctg 841 caaatgattc caaagtcttg attgataata ttccagacag ctcttcctta attgaagaga 901 caaatgaatg gcctttggaa agcttttgtg aagaactttg caatgacctt tttaatccct 961 cctgggaggt tcgacatggt gcaggcactg gacttaggga aattcttaaa gctcatggga 1021 aaagtggtgg taaaatgggt gacagcactt tagaagagat gattcagcag catcaagagt 1081 ggttggaaga cttggttatt agactccttt gtgtttttgc attagacaga tttggagact 1141 ttgtttctga tgaagttgtg gcaccagttc gtgaaacttg tgctcaaaca ttaggtgtgg 1201 ttttaaaaca catgaacgaa acaggagttc ataagactgt ggatgtgctg ctaaaattac 1261 ttacacaaga acaatgggaa gttagacatg gtggtctgct gggaataaaa tatgctttgg 1321 cagtccgtca ggatgtaatt aatactttat tgcctaaagt tttaactaga ataattgaag 1381 gactccagga tcttgatgat gatgtcagag ctgttgctgc agcatcatta gtgcctgtag 1441 tagaaagcct tgtctatctt cagacacaaa aggtgccctt cattataaat acattgtggg 1501 atgctcttct ggaattagat gatctaacag cttcaacaaa tagtattatg actctccttt 1561 catccttgtt aacttaccct caggtccaac aatgcagtat tcagcagtca ctgacagttt 1621 tagttccacg tgtctggcct tttttgcatc acactatatc atcagttcga agagcagcat 1681 tggaaactct gtttacgtta ttatcaacac aggaccagaa ctcttcatct tggcttatac 1741 ctatactgcc tgatatgctc cgacacattt ttcagttctg tgttttggaa agcagccagg 1801 aaattctgga ccttattcac aaggtttgga tggaactgtt gagtaaggct tcagttcagt 1861 atgtggtagc agctgcttgc ccatggatgg gtgcttggct ttgcttgatg atgcagcctt 1921 ctcatttacc tatcgattta aatatgttgc tagaagtaaa agctagagcc aaggaaaaaa 1981 caggtggtaa ggtgcgccaa ggccaaagcc agaataaaga agtacttcag gagtatattg 2041 caggtgccga caccatcatg gaagacccag ccaccaggga ttttgtagtt atgcgggcca 2101 gaatgatggc agccaagttg ttaggagcac tttgttgctg tatttgtgat ccaggtgtaa 2161 atgtggtaac tcaagaaatt aaaccagctg aatccctggg ccagttacta ctcttccatt 2221 tgaactccaa gtctgcttta cagaggatta gtgtagcttt ggtaatctgt gaatgggctg 2281 ctttacaaaa ggagtgtaaa gctgttacct tagctgtgca gccgcgttta cttgatatcc 2341 tttcagaaca tttatattat gacgaaattg ccgttccatt cacacgaatg cagaatgaat 2401 gtaaacaact tatatcatca ttagctgatg tacatattga agttggtaat cgagtaaaca 2461 acaatgtttt aacaatagat caagctagtg acttggtcac tactgttttt aatgaagcca 2521 catcatcttt cgatttaaat cctcaagtgt tacaacagtt agatagtaaa cgacagcagg 2581 tccaaatgac agttacagag accaaccagg agtggcaagt gttgcagttg agagtgcata 2641 cttttgctgc ctgtgcagtt gtgagcttgc agcagcttcc ggagaaatta aatcctatca 2701 taaaaccatt aatggagaca attaaaaaag aagagaatac actagtgcaa aactatgcag 2761 ctcagtgcat agctaaactc cttcagcagt gcacaacaag gacgccctgt cccaattcaa 2821 aaattattaa aaacctctgt agctcacttt gtgtggaccc atatctaact ccttgtgtca 2881 catgtccagt accaacacaa agtggccagg aaaattctaa aggatccacc tcagaaaaag 2941 atggaatgca ccatactgtc accaagcaca gaggtataat tacactctac aggcaccaga 3001 aagctgcctt tgctatcaca agtaggcgag gtcctacccc caaagcagta aaagctcaaa 3061 tagcagatct tcctgcagga agtagtggaa atattcttgt tgaacttgat gaggcccaga 3121 agccttacct ggtacaacgg agaggagctg aatttgcttt gacaactata gtaaagcatt 3181 ttggtggtga aatggcagtg aagttgccac atctctggga tgctatggtt ggcccattga 3241 ggaatacaat cgacataaat aattttgatg gaaaatccct cctggataag ggagatagcc 3301 ctgctcaaga attggtgaat tctctgcagg tttttgaaac agcagcagct tcaatggatt 3361 ctgagcttca tcccttgttg gtccagcatt tgccacatct ttatatgtgc cttcaatacc 3421 ccagtactgc agtgaggcac atggctgctc gttgtgtagg tgtcatgagc aaaatagcta 3481 ccatggaaac aatgaatatt tttttggaga aggttcttcc gtggctggga gcaattgatg 3541 acagtgtcaa acaagagggt gcaattgaag cacttgcctg tgtaatggaa caactagatg 3601 ttggtattgt tccatatatt gtccttttag ttgttcctgt attaggaaga atgagtgatc 3661 agacagacag tgtgagattc atggccacgc agagttttgc aacgctaatt agactcatgc 3721 cacttgaggc aggcattcca gaccctccaa acatgtcagc agaattaatc caattgaaag 3781 ccaaggagcg acactttttg gagcaattgt tagatgggaa aaaattggaa aattataaaa 3841 ttccagtacc aatcaatgct gaactcagaa aatatcagca ggatggtgtg aactggttag 3901 catttcttaa taagtataaa cttcatggaa ttctgtgtga tgacatgggt ttaggaaaaa 3961 ctttacagtc catctgcatt ctagcaggag atcattgtca tagggcccag gaatatgcaa 4021 gatcaaaatt agcagaatgt atgccacttc cttccttagt ggtttgtccg ccaacattaa 4081 caggccattg ggtggatgaa gtaggtaaat tttgctctag agaatatctc aacccgttgc 4141 attacactgg acctcccact gaaagaataa ggttacagca ccaagtaaaa aggcacaatc 4201 taatagtggc ttcatatgat gttgtgagga atgacataga tttctttaga aatattaaat 4261 ttaactactg cattcttgat gaaggccatg tcatcaaaaa tggaaaaaca aagttgtcaa 4321 aagcagtaaa acaactgact gctaattata ggattattct ttctggaaca ccaatccaga 4381 acaacgtttt ggagctgtgg tcattatttg atttcctcat gccaggattt ttgggtactg 4441 aacgccagtt tgctgctcga tatggtaaac ctatattagc aagtagggat gctcgaagct 4501 ccagtcgaga gcaagaagca ggtgttcttg ctatggatgc gctgcaccgc caagtactac 4561 cgtttctttt gagaagaatg aaagaagatg ttttgcagga tcttccacct aaaattattc 4621 aagactatta ttgtactctt agtcctctcc aggttcagct ctatgaagat tttgctaagt 4681 ctcgtgccaa gtgtgatgtt gatgaaacag tttcttcagc tacactttct gaagaaactg 4741 aaaaaccaaa gcttaaagct acaggccacg tattccaggc attacagtac ttacgtaaac 4801 tgtgcaacca tccagcatta gtcttaacac ctcaacatcc agaattcaag accactgccg 4861 aaaaactggc agttcagaat tcttctctac atgatattca acatgcccct aagctctcag 4921 ctttgaaaca attgctgttg gactgcggtt tgggaaatgg cagcacttcc gagagtggca 4981 cagagtctgt tgtggcccag cacaggatac tgatattctg tcagctgaaa agcatgcttg 5041 atatagtaga gcatgatctc ctcaaacctc acttgccctc tgtcacttat ttGAGATTAG 5101 ATGGCAGCAT ACCTCCTGGT CAGAGGCATT CCATTGTTTC CCGGTTTAAT AATGATCCAT 5161 CTATAGACGT TCTGTTACTT ACCACTCACG TTGGTGGCCT GGGACTTAAT TTGACAGGCG 5221 CTGACACAGT AGTATTTGTG GAGCATGACT GGAATCCTAT GCGAGATCTA CAAGCCATGG 5281 ACCGGGCCCA TCGCATTGGG CAGAAACGTG TGGTTAACGT ATACCGATTG ATAACCAGAG 5341 GAACATTGGA AGAAAAAATA ATGGGGTTGC AGAAATTCAA GATGAACATA GCGAATACTG 5401 TTATTAGCCA AGAGAATTCT AGTTTGCAGA GCATGGGGAC TGATCAGCTT CTTGATCTGT 5461 TTACTCTTGA TAAGGATGGC AAAGCAGAAA AAGCTGACAC CTCTACTTCT GGGAAAGCAA 5521 GTATGAAATC AATTCTTGAA AACCTGAGTG ATCTTTGGGA TCAAGAGCAG TATGATTCAG 5581 AGTACAGCCT GGAAAATTTT ATGCATTCTC TCAAGTTGCC AACTTTCTTG TACAAAGTGG 5641 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 5701 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 5761 ACTGACTACC TTCCTCTTTT GTCCCACGCG TTAAGTCgac aatcaacctc tggattacaa 5821 aatttgtgaa agatt