Construct: ORF TRCN0000473268

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004689.1_s317c1
Derived from:
ccsbBroadEn_01147
DNA Barcode:
GCGCCGCATAGCAAGTTATCCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PC (5091)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473268
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5091 PC pyruvate carboxylase NM_000920.4 100% 100%
2 human 5091 PC pyruvate carboxylase NM_001040716.2 100% 100%
3 human 5091 PC pyruvate carboxylase NM_022172.3 100% 100%
4 human 5091 PC pyruvate carboxylase XM_005274031.4 100% 100%
5 human 5091 PC pyruvate carboxylase XM_005274032.4 100% 100%
6 human 5091 PC pyruvate carboxylase XM_006718578.3 100% 100%
7 human 5091 PC pyruvate carboxylase XM_011545086.2 100% 100%
8 human 5091 PC pyruvate carboxylase XM_017017868.1 100% 100%
9 human 5091 PC pyruvate carboxylase XM_017017869.1 100% 100%
10 human 5091 PC pyruvate carboxylase XM_017017870.1 100% 100%
11 human 5091 PC pyruvate carboxylase XM_017017871.1 100% 100%
12 human 5091 PC pyruvate carboxylase XM_017017872.2 100% 100%
13 human 5091 PC pyruvate carboxylase XM_011545087.2 62.3% 61.6% (many diffs)
14 human 5091 PC pyruvate carboxylase XM_006718579.3 56.9% 56.9% 0_1ins1521
15 mouse 18563 Pcx pyruvate carboxylase NM_008797.3 89.1% 96.7% (many diffs)
16 mouse 18563 Pcx pyruvate carboxylase XM_006531678.3 89.1% 96.7% (many diffs)
17 mouse 18563 Pcx pyruvate carboxylase NM_001162946.1 89% 96.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3600
ORF length:
3534
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gaagttccga acagtccatg ggggcctgag gctcctggga atccgccgaa
121cctccaccgc ccccgctgcc tccccaaatg tccggcgcct ggagtataag cccatcaaga
181aagtcatggt ggccaacaga ggtgagattg ccatccgtgt gttccgggcc tgcacggagc
241tgggcatccg caccgtagcc atctactctg agcaggacac gggccagatg caccggcaga
301aagcagatga agcctatctc atcggccgcg gcctggcccc cgtgcaggcc tacctgcaca
361tcccagacat catcaaggtg gccaaggaga acaacgtaga tgcagtgcac cctggctacg
421ggttcctctc tgagcgagcg gacttcgccc aggcctgcca ggatgcaggg gtccggttta
481ttgggccaag cccagaagtg gtccgcaaga tgggagacaa ggtggaggcc cgggccatcg
541ccattgctgc gggtgttccc gttgtccctg gcacagatgc ccccatcacg tccctgcatg
601aggcccacga gttctccaac acctacggct tccccatcat cttcaaggcg gcctatgggg
661gtggagggcg tggcatgagg gtggtgcaca gctacgagga gctggaggag aattacaccc
721gggcctactc agaggctctg gccgcctttg ggaatggggc gctgtttgtg gagaagttca
781tcgagaagcc acggcacatc gaggtgcaga tcttggggga ccagtatggg aacatcctgc
841acctgtacga gcgagactgc tccatccagc ggcggcacca gaaggtggtc gagattgccc
901ccgccgccca cctggacccg cagcttcgga ctcggctcac cagcgactct gtgaaactcg
961ctaaacaggt gggctacgag aacgcaggca ccgtggagtt cctggtggac aggcacggca
1021agcactactt catcgaggtc aactcccgcc tgcaggtgga gcacacggtc acagaggaga
1081tcaccgacgt agacctggtc catgctcaga tccacgtggc tgagggcagg agcctacccg
1141acctgggcct gcggcaggag aacatccgca tcaacgggtg tgccatccag tgccgggtca
1201ccaccgagga ccccgcgcgc agcttccagc cggacaccgg ccgcattgag gtgttccgga
1261gcggagaggg catgggcatc cgcctggata atgcttccgc cttccaagga gccgtcatct
1321cgccccacta cgactccctg ctggtcaaag tcattgccca cggcaaagac caccccacgg
1381ccgccaccaa gatgagcagg gcccttgcgg agttccgcgt ccgaggtgtg aagaccaaca
1441tcgccttcct gcagaatgtg ctcaacaacc agcagttcct ggcaggcact gtggacaccc
1501agttcatcga cgagaaccca gagctgttcc agctgcggcc tgcacagaac cgggcccaaa
1561agctgttgca ctacctcggc catgtcatgg taaacggtcc aaccaccccg attcccgtca
1621aggccagccc cagccccacg gaccccgttg tccctgcagt gcccataggc ccgcccccgg
1681ctggtttcag agacatcctg ctgcgagagg ggcctgaggg ctttgctcga gctgtgcgga
1741accacccggg gctgctgctg atggacacga ccttcaggga cgcccaccag tcactgctgg
1801ccactcgtgt gcgcacccac gatctcaaaa agatcgcccc ctatgttgcc cacaacttca
1861gcaagctctt cagcatggag aactggggag gagccacgtt tgacgtcgcc atgcgcttcc
1921tgtatgagtg cccctggcgg cggctgcagg agctccggga gctcatcccc aacatccctt
1981tccagatgct gctgcggggg gccaatgctg tgggctacac caactaccca gacaacgtgg
2041tcttcaagtt ctgtgaagtg gccaaagaga atggcatgga tgtcttccgt gtgtttgact
2101ccctcaacta cttgcccaac atgctgctgg gcatggaggc ggcaggaagt gccggaggcg
2161tggtggaggc tgccatctca tacacgggcg acgtggccga ccccagccgc accaagtact
2221cactgcagta ctacatgggc ttggccgaag agctggtgcg agctggcacc cacatcctgt
2281gcatcaagga catggccggg ctgctgaagc ccacggcctg caccatgctg gtcagctccc
2341tccgggaccg cttccccgac ctcccactgc acatccacac ccacgacacg tcaggggcag
2401gcgtggcagc catgctggcc tgtgcccagg ctggagctga tgtggtggat gtggcagctg
2461attccatgtc tgggatgact tcacagccca gcatgggggc cctggtggcc tgtaccagag
2521ggactcccct ggacacagag gtgcccatgg agcgcgtgtt tgactacagt gagtactggg
2581agggggctcg gggactgtac gcggccttcg actgcacggc caccatgaag tctggcaact
2641cggacgtgta tgaaaatgag atcccagggg gccagtacac caacctgcac ttccaggccc
2701acagcatggg gcttggctcc aagttcaagg aggtcaagaa ggcctatgtg gaggccaacc
2761agatgctggg cgatctcatc aaggtgacgc cctcctccaa gatcgtgggg gacctggccc
2821agtttatggt gcagaatgga ttgagccggg cagaggccga agctcaggcg gaagagctgt
2881cctttccccg ctccgtggtg gagttcctgc agggctacat cggtgtcccc catggggggt
2941tccccgaacc ctttcgctct aaggtactga aggacctgcc aagggtggag gggcggcctg
3001gagcctccct ccctcccctg gatctgcagg cactggagaa ggagctggta gaccggcatg
3061gggaggaggt gacgccggaa gatgtgctcT CAGCAGCTAT GTACCCCGAT GTGTTTGCCC
3121ACTTCAAGGA CTTCACTGCC ACCTTTGGCC CCCTGGATAG CCTGAATACT CGCCTCTTCC
3181TGCAGGGACC CAAGATCGCA GAGGAGTTTG AGGTGGAGCT GGAGCGGGGC AAGACGCTGC
3241ACATCAAAGC CCTGGCCGTG AGCGACCTGA ACCGGGCCGG CCAGAGGCAG GTCTTCTTTG
3301AGCTCAATGG GCAGCTGCGG TCCATCTTGG TCAAGGACAC CCAGGCCATG AAGGAGATGC
3361ACTTCCACCC CAAGGCCCTA AAGGACGTGA AGGGCCAGAT CGGGGCGCCC ATGCCTGGGA
3421AGGTGATAGA CATCAAAGTG GTGGCAGGGG CCAAGGTGGC CAAGGGCCAG CCCCTGTGTG
3481TGCTCAGTGC CATGAAGATG GAGACTGTGG TGACCTCACC CATGGAGGGT ACTGTCCGCA
3541AGGTTCATGT GACCAAGGAC ATGACACTGG AAGGTGACGA CCTCATCCTG GAGATCGAGT
3601GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
3661GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
3721TTTATATATC TTGTGGAAAG GACGAGCGCC GCATAGCAAG TTATCCAATA CGCGTTAAGT
3781Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.