Construct: ORF TRCN0000473268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004689.1_s317c1
- Derived from:
- ccsbBroadEn_01147
- DNA Barcode:
- GCGCCGCATAGCAAGTTATCCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PC (5091)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473268
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5091 | PC | pyruvate carboxylase | NM_000920.4 | 100% | 100% | |
| 2 | human | 5091 | PC | pyruvate carboxylase | NM_001040716.2 | 100% | 100% | |
| 3 | human | 5091 | PC | pyruvate carboxylase | NM_022172.3 | 100% | 100% | |
| 4 | human | 5091 | PC | pyruvate carboxylase | XM_005274031.4 | 100% | 100% | |
| 5 | human | 5091 | PC | pyruvate carboxylase | XM_005274032.4 | 100% | 100% | |
| 6 | human | 5091 | PC | pyruvate carboxylase | XM_006718578.3 | 100% | 100% | |
| 7 | human | 5091 | PC | pyruvate carboxylase | XM_011545086.2 | 100% | 100% | |
| 8 | human | 5091 | PC | pyruvate carboxylase | XM_017017868.1 | 100% | 100% | |
| 9 | human | 5091 | PC | pyruvate carboxylase | XM_017017869.1 | 100% | 100% | |
| 10 | human | 5091 | PC | pyruvate carboxylase | XM_017017870.1 | 100% | 100% | |
| 11 | human | 5091 | PC | pyruvate carboxylase | XM_017017871.1 | 100% | 100% | |
| 12 | human | 5091 | PC | pyruvate carboxylase | XM_017017872.2 | 100% | 100% | |
| 13 | human | 5091 | PC | pyruvate carboxylase | XM_011545087.2 | 62.3% | 61.6% | (many diffs) |
| 14 | human | 5091 | PC | pyruvate carboxylase | XM_006718579.3 | 56.9% | 56.9% | 0_1ins1521 |
| 15 | mouse | 18563 | Pcx | pyruvate carboxylase | NM_008797.3 | 89.1% | 96.7% | (many diffs) |
| 16 | mouse | 18563 | Pcx | pyruvate carboxylase | XM_006531678.3 | 89.1% | 96.7% | (many diffs) |
| 17 | mouse | 18563 | Pcx | pyruvate carboxylase | NM_001162946.1 | 89% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3600
- ORF length:
- 3534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaagttccga acagtccatg ggggcctgag gctcctggga atccgccgaa 121 cctccaccgc ccccgctgcc tccccaaatg tccggcgcct ggagtataag cccatcaaga 181 aagtcatggt ggccaacaga ggtgagattg ccatccgtgt gttccgggcc tgcacggagc 241 tgggcatccg caccgtagcc atctactctg agcaggacac gggccagatg caccggcaga 301 aagcagatga agcctatctc atcggccgcg gcctggcccc cgtgcaggcc tacctgcaca 361 tcccagacat catcaaggtg gccaaggaga acaacgtaga tgcagtgcac cctggctacg 421 ggttcctctc tgagcgagcg gacttcgccc aggcctgcca ggatgcaggg gtccggttta 481 ttgggccaag cccagaagtg gtccgcaaga tgggagacaa ggtggaggcc cgggccatcg 541 ccattgctgc gggtgttccc gttgtccctg gcacagatgc ccccatcacg tccctgcatg 601 aggcccacga gttctccaac acctacggct tccccatcat cttcaaggcg gcctatgggg 661 gtggagggcg tggcatgagg gtggtgcaca gctacgagga gctggaggag aattacaccc 721 gggcctactc agaggctctg gccgcctttg ggaatggggc gctgtttgtg gagaagttca 781 tcgagaagcc acggcacatc gaggtgcaga tcttggggga ccagtatggg aacatcctgc 841 acctgtacga gcgagactgc tccatccagc ggcggcacca gaaggtggtc gagattgccc 901 ccgccgccca cctggacccg cagcttcgga ctcggctcac cagcgactct gtgaaactcg 961 ctaaacaggt gggctacgag aacgcaggca ccgtggagtt cctggtggac aggcacggca 1021 agcactactt catcgaggtc aactcccgcc tgcaggtgga gcacacggtc acagaggaga 1081 tcaccgacgt agacctggtc catgctcaga tccacgtggc tgagggcagg agcctacccg 1141 acctgggcct gcggcaggag aacatccgca tcaacgggtg tgccatccag tgccgggtca 1201 ccaccgagga ccccgcgcgc agcttccagc cggacaccgg ccgcattgag gtgttccgga 1261 gcggagaggg catgggcatc cgcctggata atgcttccgc cttccaagga gccgtcatct 1321 cgccccacta cgactccctg ctggtcaaag tcattgccca cggcaaagac caccccacgg 1381 ccgccaccaa gatgagcagg gcccttgcgg agttccgcgt ccgaggtgtg aagaccaaca 1441 tcgccttcct gcagaatgtg ctcaacaacc agcagttcct ggcaggcact gtggacaccc 1501 agttcatcga cgagaaccca gagctgttcc agctgcggcc tgcacagaac cgggcccaaa 1561 agctgttgca ctacctcggc catgtcatgg taaacggtcc aaccaccccg attcccgtca 1621 aggccagccc cagccccacg gaccccgttg tccctgcagt gcccataggc ccgcccccgg 1681 ctggtttcag agacatcctg ctgcgagagg ggcctgaggg ctttgctcga gctgtgcgga 1741 accacccggg gctgctgctg atggacacga ccttcaggga cgcccaccag tcactgctgg 1801 ccactcgtgt gcgcacccac gatctcaaaa agatcgcccc ctatgttgcc cacaacttca 1861 gcaagctctt cagcatggag aactggggag gagccacgtt tgacgtcgcc atgcgcttcc 1921 tgtatgagtg cccctggcgg cggctgcagg agctccggga gctcatcccc aacatccctt 1981 tccagatgct gctgcggggg gccaatgctg tgggctacac caactaccca gacaacgtgg 2041 tcttcaagtt ctgtgaagtg gccaaagaga atggcatgga tgtcttccgt gtgtttgact 2101 ccctcaacta cttgcccaac atgctgctgg gcatggaggc ggcaggaagt gccggaggcg 2161 tggtggaggc tgccatctca tacacgggcg acgtggccga ccccagccgc accaagtact 2221 cactgcagta ctacatgggc ttggccgaag agctggtgcg agctggcacc cacatcctgt 2281 gcatcaagga catggccggg ctgctgaagc ccacggcctg caccatgctg gtcagctccc 2341 tccgggaccg cttccccgac ctcccactgc acatccacac ccacgacacg tcaggggcag 2401 gcgtggcagc catgctggcc tgtgcccagg ctggagctga tgtggtggat gtggcagctg 2461 attccatgtc tgggatgact tcacagccca gcatgggggc cctggtggcc tgtaccagag 2521 ggactcccct ggacacagag gtgcccatgg agcgcgtgtt tgactacagt gagtactggg 2581 agggggctcg gggactgtac gcggccttcg actgcacggc caccatgaag tctggcaact 2641 cggacgtgta tgaaaatgag atcccagggg gccagtacac caacctgcac ttccaggccc 2701 acagcatggg gcttggctcc aagttcaagg aggtcaagaa ggcctatgtg gaggccaacc 2761 agatgctggg cgatctcatc aaggtgacgc cctcctccaa gatcgtgggg gacctggccc 2821 agtttatggt gcagaatgga ttgagccggg cagaggccga agctcaggcg gaagagctgt 2881 cctttccccg ctccgtggtg gagttcctgc agggctacat cggtgtcccc catggggggt 2941 tccccgaacc ctttcgctct aaggtactga aggacctgcc aagggtggag gggcggcctg 3001 gagcctccct ccctcccctg gatctgcagg cactggagaa ggagctggta gaccggcatg 3061 gggaggaggt gacgccggaa gatgtgctcT CAGCAGCTAT GTACCCCGAT GTGTTTGCCC 3121 ACTTCAAGGA CTTCACTGCC ACCTTTGGCC CCCTGGATAG CCTGAATACT CGCCTCTTCC 3181 TGCAGGGACC CAAGATCGCA GAGGAGTTTG AGGTGGAGCT GGAGCGGGGC AAGACGCTGC 3241 ACATCAAAGC CCTGGCCGTG AGCGACCTGA ACCGGGCCGG CCAGAGGCAG GTCTTCTTTG 3301 AGCTCAATGG GCAGCTGCGG TCCATCTTGG TCAAGGACAC CCAGGCCATG AAGGAGATGC 3361 ACTTCCACCC CAAGGCCCTA AAGGACGTGA AGGGCCAGAT CGGGGCGCCC ATGCCTGGGA 3421 AGGTGATAGA CATCAAAGTG GTGGCAGGGG CCAAGGTGGC CAAGGGCCAG CCCCTGTGTG 3481 TGCTCAGTGC CATGAAGATG GAGACTGTGG TGACCTCACC CATGGAGGGT ACTGTCCGCA 3541 AGGTTCATGT GACCAAGGAC ATGACACTGG AAGGTGACGA CCTCATCCTG GAGATCGAGT 3601 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3721 TTTATATATC TTGTGGAAAG GACGAGCGCC GCATAGCAAG TTATCCAATA CGCGTTAAGT 3781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt