Construct: ORF TRCN0000473272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017629.1_s317c1
- Derived from:
- ccsbBroadEn_02057
- DNA Barcode:
- CCGAGGCGTGAGTGCACACGACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P4HA2 (8974)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473272
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001017974.2 | 100% | 100% | |
| 2 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001142598.2 | 100% | 100% | |
| 3 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365678.2 | 100% | 100% | |
| 4 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365679.2 | 100% | 100% | |
| 5 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365680.2 | 100% | 100% | |
| 6 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001142599.2 | 97.5% | 97.7% | (many diffs) |
| 7 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365677.2 | 97.5% | 97.7% | (many diffs) |
| 8 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_004199.3 | 97.5% | 97.7% | (many diffs) |
| 9 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272117.4 | 97.5% | 97.7% | (many diffs) |
| 10 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272118.4 | 97.5% | 97.7% | (many diffs) |
| 11 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272119.4 | 97.5% | 97.7% | (many diffs) |
| 12 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_005272120.4 | 97.5% | 97.7% | (many diffs) |
| 13 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_006714728.3 | 97.5% | 97.7% | (many diffs) |
| 14 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | NM_001365681.2 | 88.9% | 88.9% | 903_904ins177 |
| 15 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_024446256.1 | 88.9% | 88.9% | 903_904ins177 |
| 16 | human | 8974 | P4HA2 | prolyl 4-hydroxylase subuni... | XM_024446255.1 | 86.6% | 86.7% | (many diffs) |
| 17 | mouse | 18452 | P4ha2 | procollagen-proline, 2-oxog... | NM_001136076.2 | 87.1% | 92.3% | (many diffs) |
| 18 | mouse | 18452 | P4ha2 | procollagen-proline, 2-oxog... | NM_011031.2 | 85.5% | 90.1% | (many diffs) |
| 19 | mouse | 18452 | P4ha2 | procollagen-proline, 2-oxog... | XM_006532452.2 | 83.7% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1665
- ORF length:
- 1599
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa actctgggtg tctgcattgc tgatggcctg gtttggtgtc ctgagctgtg 121 tgcaggccga attcttcacc tctattgggc acatgactga cctgatttat gcagagaaag 181 agctggtgca gtctctgaaa gagtacatcc ttgtggagga agccaagctt tccaagatta 241 agagctgggc caacaaaatg gaagccttga ctagcaagtc agctgctgat gctgagggct 301 acctggctca ccctgtgaat gcctacaaac tggtgaagcg gctaaacaca gactggcctg 361 cgctggagga ccttgtcctg caggactcag ctgcaggttt tatcgccaac ctctctgtgc 421 agcggcagtt cttccccact gatgaggacg agataggagc tgccaaagcc ctgatgagac 481 ttcaggacac atacaggctg gacccaggca caatttccag aggggaactt ccaggaacca 541 agtaccaggc aatgctgagt gtggatgact gctttgggat gggccgctcg gcctacaatg 601 aaggggacta ttatcatacg gtgttgtgga tggagcaggt gctaaagcag cttgatgccg 661 gggaggaggc caccacaacc aagtcacagg tgctggacta cctcagctat gctgtcttcc 721 agttgggtga tctgcaccgt gccctggagc tcacccgccg cctgctctcc cttgacccaa 781 gccacgaacg agctggaggg aatctgcggt actttgagca gttattggag gaagagagag 841 aaaaaacgtt aacaaatcag acagaagctg agctagcaac cccagaaggc atctatgaga 901 ggcctgtgga ctacctgcct gagagggatg tttacgagag cctctgtcgt ggggagggtg 961 tcaaactgac accccgtaga cagaagaggc ttttctgtag gtaccaccat ggcaacaggg 1021 ccccacagct gctcattgcc cccttcaaag aggaggacga gtgggacagc ccgcacatcg 1081 tcaggtacta cgatgtcatg tctgatgagg aaatcgagag gatcaaggag atcgcaaaac 1141 CTAAACTTGC ACGAGCCACC GTTCGTGATC CCAAGACAGG AGTCCTCACT GTCGCCAGCT 1201 ACCGGGTTTC CAAAAGCTCC TGGCTAGAGG AAGATGATGA CCCTGTTGTG GCCCGAGTAA 1261 ATCGTCGGAT GCAGCATATC ACAGGGTTAA CAGTAAAGAC TGCAGAATTG TTACAGGTTG 1321 CAAATTATGG AGTGGGAGGA CAGTATGAAC CGCACTTCGA CTTCTCTAGG CGACCTTTTG 1381 ACAGCGGCCT CAAAACAGAG GGGAATAGGT TAGCGACGTT TCTTAACTAC ATGAGTGATG 1441 TAGAAGCTGG TGGTGCCACC GTCTTCCCTG ATCTGGGGGC TGCAATTTGG CCTAAGAAGG 1501 GTACAGCTGT GTTCTGGTAC AACCTCTTGC GGAGCGGGGA AGGTGACTAC CGAACAAGAC 1561 ATGCTGCCTG CCCTGTGCTT GTGGGCTGCA AGTGGGTCTC CAATAAGTGG TTCCATGAAC 1621 GAGGACAGGA GTTCTTGAGA CCTTGTGGAT CAACAGAAGT TGACTGCCCA ACTTTCTTGT 1681 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1741 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1801 GAAAGGACGA CCGAGGCGTG AGTGCACACG ACATACGCGT TAAGTCgaca atcaacctct 1861 ggattacaaa atttgtgaaa gatt