Construct: ORF TRCN0000473305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013225.1_s317c1
- Derived from:
- ccsbBroadEn_00454
- DNA Barcode:
- TCCTGATCAGAATGGATACCCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNMT3A (1788)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NM_022552.4 | 100% | 100% | |
| 2 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NM_175629.2 | 100% | 100% | |
| 3 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_005264175.5 | 100% | 100% | |
| 4 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_017003526.1 | 100% | 100% | |
| 5 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_011532662.2 | 94.1% | 93.6% | (many diffs) |
| 6 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_011532663.2 | 86.9% | 85.4% | 0_1ins268;180_282del |
| 7 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_011532664.2 | 85.3% | 84.8% | (many diffs) |
| 8 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NM_001320893.1 | 81.9% | 77.8% | (many diffs) |
| 9 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NM_153759.3 | 78.3% | 77% | (many diffs) |
| 10 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_011532666.2 | 78% | 76.5% | (many diffs) |
| 11 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_005264177.4 | 75.5% | 75.5% | 0_1ins669 |
| 12 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_011532667.3 | 75.5% | 75.5% | 0_1ins669 |
| 13 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XM_017003527.1 | 75.5% | 75.5% | 0_1ins669 |
| 14 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | XR_001738657.1 | 61.7% | 1_277del;2683_2684ins70;2944_4249del | |
| 15 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NR_135490.2 | 28.5% | 1_231del;2554_2752del;3167_9574del | |
| 16 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NM_001320892.2 | 17.6% | 16.5% | (many diffs) |
| 17 | human | 1788 | DNMT3A | DNA methyltransferase 3 alpha | NM_175630.1 | 17.6% | 16.5% | (many diffs) |
| 18 | mouse | 13435 | Dnmt3a | DNA methyltransferase 3A | NM_001271753.1 | 90.2% | 95.9% | (many diffs) |
| 19 | mouse | 13435 | Dnmt3a | DNA methyltransferase 3A | NM_007872.4 | 90.2% | 95.9% | (many diffs) |
| 20 | mouse | 13435 | Dnmt3a | DNA methyltransferase 3A | XM_006514953.3 | 90.2% | 95.9% | (many diffs) |
| 21 | mouse | 13435 | Dnmt3a | DNA methyltransferase 3A | XM_006514956.3 | 90.2% | 95.9% | (many diffs) |
| 22 | mouse | 13435 | Dnmt3a | DNA methyltransferase 3A | NM_153743.4 | 69% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2802
- ORF length:
- 2736
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgccatgccc tccagcggcc ccggggacac cagcagctct gctgcggagc 121 gggaggagga ccgaaaggac ggagaggagc aggaggagcc gcgtggcaag gaggagcgcc 181 aagagcccag caccacggca cggaaggtgg ggcggcctgg gaggaagcgc aagcaccccc 241 cggtggaaag cggtgacacg ccaaaggacc ctgcggtgat ctccaagtcc ccatccatgg 301 cccaggactc aggcgcctca gagctattac ccaatgggga cttggagaag cggagtgagc 361 cccagccaga ggaggggagc cctgctgggg ggcagaaggg cggggcccca gcagagggag 421 agggtgcagc tgagaccctg cctgaagcct caagagcagt ggaaaatggc tgctgcaccc 481 ccaaggaggg ccgaggagcc cctgcagaag cgggcaaaga acagaaggag accaacatcg 541 aatccatgaa aatggagggc tcccggggcc ggctgcgggg tggcttgggc tgggagtcca 601 gcctccgtca gcggcccatg ccgaggctca ccttccaggc gggggacccc tactacatca 661 gcaagcgcaa gcgggacgag tggctggcac gctggaaaag ggaggctgag aagaaagcca 721 aggtcattgc aggaatgaat gctgtggaag aaaaccaggg gcccggggag tctcagaagg 781 tggaggaggc cagccctcct gctgtgcagc agcccactga ccccgcatcc cccactgtgg 841 ctaccacgcc tgagcccgtg gggtccgatg ctggggacaa gaatgccacc aaagcaggcg 901 atgacgagcc agagtacgag gacggccggg gctttggcat tggggagctg gtgtggggga 961 aactgcgggg cttctcctgg tggccaggcc gcattgtgtc ttggtggatg acgggccgga 1021 gccgagcagc tgaaggcacc cgctgggtca tgtggttcgg agacggcaaa ttctcagtgg 1081 tgtgtgttga gaagctgatg ccgctgagct cgttttgcag tgcgttccac caggccacgt 1141 acaacaagca gcccatgtac cgcaaagcca tctacgaggt cctgcaggtg gccagcagcc 1201 gcgcggggaa gctgttcccg gtgtgccacg acagcgatga gagtgacact gccaaggccg 1261 tggaggtgca gaacaagccc atgattgaat gggccctggg gggcttccag ccttctggcc 1321 ctaagggcct ggagccacca gaagaagaga agaatcccta caaagaagtg tacacggaca 1381 tgtgggtgga acctgaggca gctgcctacg caccacctcc accagccaaa aagccccgga 1441 agagcacagc ggagaagccc aaggtcaagg agattattga tgagcgcaca agagagcggc 1501 tggtgtacga ggtgcggcag aagtgccgga acattgagga catctgcatc tcctgtggga 1561 gcctcaatgt taccctggaa caccccctct tcgttggagg aatgtgccaa aactgcaaga 1621 actgctttct ggagtgtgcg taccagtacg acgacgacgg ctaccagtcc tactgcacca 1681 tctgctgtgg gggccgtgag gtgctcatgt gcggaaacaa caactgctgc aggtgctttt 1741 gcgtggagtg tgtggacctc ttggtggggc cgggggctgc ccaggcagcc attaaggaag 1801 acccctggaa ctgctacatg tgcgggcaca agggtaccta cgggctgctg cggcggcgag 1861 aggactggcc ctcccggctc cagatgttct tcgctaataa ccacgaccag gaatttgacc 1921 ctccaaaggt ttacccacct gtcccagctg agaagaggaa gcccatccgg gtgctgtctc 1981 tctttgatgg aatcgctaca gggctcctgg tgctgaagga cttgggcatt caggtggacc 2041 gctacattgc ctcggaggtg tgtgaggact ccatcacggt gggcatggtg cggcaccagg 2101 ggaagatcat gtacgtcggg gacgtccgca gcgtcacaca gaagcatatc caggagtggg 2161 gcccattcga tctggtgatt gggggcagtc cctgcaatga cctctccatc gtcaaccctg 2221 ctcgcaaggg cctctacgag ggcactggcc ggctcttctt tgagttctac cgcctcctgc 2281 atgatgcgcg gcccaaggag ggagatgatc gccccttctt ctggctcttt gagaatgtgg 2341 tggccatggg cgttagtgac aagagggaca tctcgcgatt tctcgagtcc aaccctgtga 2401 tgattgatgc caaagaagtg TCAGCTGCAC ACAGGGCCCG CTACTTCTGG GGTAACCTTC 2461 CCGGTATGAA CAGGCCGTTG GCATCCACTG TGAATGATAA GCTGGAGCTG CAGGAGTGTC 2521 TGGAGCATGG CAGGATAGCC AAGTTCAGCA AAGTGAGGAC CATTACTACG AGGTCAAACT 2581 CCATAAAGCA GGGCAAAGAC CAGCATTTTC CTGTCTTCAT GAATGAGAAA GAGGACATCT 2641 TATGGTGCAC TGAAATGGAA AGGGTATTTG GTTTCCCAGT CCACTATACT GACGTCTCCA 2701 ACATGAGCCG CTTGGCGAGG CAGAGACTGC TGGGCCGGTC ATGGAGCGTG CCAGTCATCC 2761 GCCACCTCTT CGCTCCGCTG AAGGAGTATT TTGCGTGTGT GTACCCAACT TTCTTGTACA 2821 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2881 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2941 AGGACGATCC TGATCAGAAT GGATACCCTA CACGCGTTAA GTCgacaatc aacctctgga 3001 ttacaaaatt tgtgaaagat t