Construct: ORF TRCN0000473305

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013225.1_s317c1
Derived from:
ccsbBroadEn_00454
DNA Barcode:
TCCTGATCAGAATGGATACCCTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DNMT3A (1788)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473305
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1788 DNMT3A DNA methyltransferase 3 alpha NM_022552.4 100% 100%
2 human 1788 DNMT3A DNA methyltransferase 3 alpha NM_175629.2 100% 100%
3 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_005264175.5 100% 100%
4 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_017003526.1 100% 100%
5 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_011532662.2 94.1% 93.6% (many diffs)
6 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_011532663.2 86.9% 85.4% 0_1ins268;180_282del
7 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_011532664.2 85.3% 84.8% (many diffs)
8 human 1788 DNMT3A DNA methyltransferase 3 alpha NM_001320893.1 81.9% 77.8% (many diffs)
9 human 1788 DNMT3A DNA methyltransferase 3 alpha NM_153759.3 78.3% 77% (many diffs)
10 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_011532666.2 78% 76.5% (many diffs)
11 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_005264177.4 75.5% 75.5% 0_1ins669
12 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_011532667.3 75.5% 75.5% 0_1ins669
13 human 1788 DNMT3A DNA methyltransferase 3 alpha XM_017003527.1 75.5% 75.5% 0_1ins669
14 human 1788 DNMT3A DNA methyltransferase 3 alpha XR_001738657.1 61.7% 1_277del;2683_2684ins70;2944_4249del
15 human 1788 DNMT3A DNA methyltransferase 3 alpha NR_135490.2 28.5% 1_231del;2554_2752del;3167_9574del
16 human 1788 DNMT3A DNA methyltransferase 3 alpha NM_001320892.2 17.6% 16.5% (many diffs)
17 human 1788 DNMT3A DNA methyltransferase 3 alpha NM_175630.1 17.6% 16.5% (many diffs)
18 mouse 13435 Dnmt3a DNA methyltransferase 3A NM_001271753.1 90.2% 95.9% (many diffs)
19 mouse 13435 Dnmt3a DNA methyltransferase 3A NM_007872.4 90.2% 95.9% (many diffs)
20 mouse 13435 Dnmt3a DNA methyltransferase 3A XM_006514953.3 90.2% 95.9% (many diffs)
21 mouse 13435 Dnmt3a DNA methyltransferase 3A XM_006514956.3 90.2% 95.9% (many diffs)
22 mouse 13435 Dnmt3a DNA methyltransferase 3A NM_153743.4 69% 74.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2802
ORF length:
2736
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc cgccatgccc tccagcggcc ccggggacac cagcagctct gctgcggagc
121gggaggagga ccgaaaggac ggagaggagc aggaggagcc gcgtggcaag gaggagcgcc
181aagagcccag caccacggca cggaaggtgg ggcggcctgg gaggaagcgc aagcaccccc
241cggtggaaag cggtgacacg ccaaaggacc ctgcggtgat ctccaagtcc ccatccatgg
301cccaggactc aggcgcctca gagctattac ccaatgggga cttggagaag cggagtgagc
361cccagccaga ggaggggagc cctgctgggg ggcagaaggg cggggcccca gcagagggag
421agggtgcagc tgagaccctg cctgaagcct caagagcagt ggaaaatggc tgctgcaccc
481ccaaggaggg ccgaggagcc cctgcagaag cgggcaaaga acagaaggag accaacatcg
541aatccatgaa aatggagggc tcccggggcc ggctgcgggg tggcttgggc tgggagtcca
601gcctccgtca gcggcccatg ccgaggctca ccttccaggc gggggacccc tactacatca
661gcaagcgcaa gcgggacgag tggctggcac gctggaaaag ggaggctgag aagaaagcca
721aggtcattgc aggaatgaat gctgtggaag aaaaccaggg gcccggggag tctcagaagg
781tggaggaggc cagccctcct gctgtgcagc agcccactga ccccgcatcc cccactgtgg
841ctaccacgcc tgagcccgtg gggtccgatg ctggggacaa gaatgccacc aaagcaggcg
901atgacgagcc agagtacgag gacggccggg gctttggcat tggggagctg gtgtggggga
961aactgcgggg cttctcctgg tggccaggcc gcattgtgtc ttggtggatg acgggccgga
1021gccgagcagc tgaaggcacc cgctgggtca tgtggttcgg agacggcaaa ttctcagtgg
1081tgtgtgttga gaagctgatg ccgctgagct cgttttgcag tgcgttccac caggccacgt
1141acaacaagca gcccatgtac cgcaaagcca tctacgaggt cctgcaggtg gccagcagcc
1201gcgcggggaa gctgttcccg gtgtgccacg acagcgatga gagtgacact gccaaggccg
1261tggaggtgca gaacaagccc atgattgaat gggccctggg gggcttccag ccttctggcc
1321ctaagggcct ggagccacca gaagaagaga agaatcccta caaagaagtg tacacggaca
1381tgtgggtgga acctgaggca gctgcctacg caccacctcc accagccaaa aagccccgga
1441agagcacagc ggagaagccc aaggtcaagg agattattga tgagcgcaca agagagcggc
1501tggtgtacga ggtgcggcag aagtgccgga acattgagga catctgcatc tcctgtggga
1561gcctcaatgt taccctggaa caccccctct tcgttggagg aatgtgccaa aactgcaaga
1621actgctttct ggagtgtgcg taccagtacg acgacgacgg ctaccagtcc tactgcacca
1681tctgctgtgg gggccgtgag gtgctcatgt gcggaaacaa caactgctgc aggtgctttt
1741gcgtggagtg tgtggacctc ttggtggggc cgggggctgc ccaggcagcc attaaggaag
1801acccctggaa ctgctacatg tgcgggcaca agggtaccta cgggctgctg cggcggcgag
1861aggactggcc ctcccggctc cagatgttct tcgctaataa ccacgaccag gaatttgacc
1921ctccaaaggt ttacccacct gtcccagctg agaagaggaa gcccatccgg gtgctgtctc
1981tctttgatgg aatcgctaca gggctcctgg tgctgaagga cttgggcatt caggtggacc
2041gctacattgc ctcggaggtg tgtgaggact ccatcacggt gggcatggtg cggcaccagg
2101ggaagatcat gtacgtcggg gacgtccgca gcgtcacaca gaagcatatc caggagtggg
2161gcccattcga tctggtgatt gggggcagtc cctgcaatga cctctccatc gtcaaccctg
2221ctcgcaaggg cctctacgag ggcactggcc ggctcttctt tgagttctac cgcctcctgc
2281atgatgcgcg gcccaaggag ggagatgatc gccccttctt ctggctcttt gagaatgtgg
2341tggccatggg cgttagtgac aagagggaca tctcgcgatt tctcgagtcc aaccctgtga
2401tgattgatgc caaagaagtg TCAGCTGCAC ACAGGGCCCG CTACTTCTGG GGTAACCTTC
2461CCGGTATGAA CAGGCCGTTG GCATCCACTG TGAATGATAA GCTGGAGCTG CAGGAGTGTC
2521TGGAGCATGG CAGGATAGCC AAGTTCAGCA AAGTGAGGAC CATTACTACG AGGTCAAACT
2581CCATAAAGCA GGGCAAAGAC CAGCATTTTC CTGTCTTCAT GAATGAGAAA GAGGACATCT
2641TATGGTGCAC TGAAATGGAA AGGGTATTTG GTTTCCCAGT CCACTATACT GACGTCTCCA
2701ACATGAGCCG CTTGGCGAGG CAGAGACTGC TGGGCCGGTC ATGGAGCGTG CCAGTCATCC
2761GCCACCTCTT CGCTCCGCTG AAGGAGTATT TTGCGTGTGT GTACCCAACT TTCTTGTACA
2821AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2881AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2941AGGACGATCC TGATCAGAAT GGATACCCTA CACGCGTTAA GTCgacaatc aacctctgga
3001ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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