Construct: ORF TRCN0000473310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010973.1_s317c1
- Derived from:
- ccsbBroadEn_12571
- DNA Barcode:
- CGGCGCGGGCATTGTATCTGGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OBI1 (79596)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473310
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79596 | OBI1 | ORC ubiquitin ligase 1 | XM_011535225.1 | 83.2% | 82.8% | 1601_1602insT;1623_1947del |
| 2 | human | 79596 | OBI1 | ORC ubiquitin ligase 1 | XM_024449410.1 | 83.2% | 82.8% | 1601_1602insT;1623_1947del |
| 3 | human | 79596 | OBI1 | ORC ubiquitin ligase 1 | NM_024546.4 | 74.4% | 74.1% | 1_231del;1832_1833insT;1854_2178del |
| 4 | mouse | 72486 | Rnf219 | ring finger protein 219 | XM_011245206.2 | 19.4% | 19.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1689
- ORF length:
- 1623
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct aagccatacg gtcaggaagc atcttcggaa aactagactt gaattactac 121 acaaagaata tgaggacgaa atagattgtt tacagaaaga agtagaagag cttaagagta 181 aaaatctcag cttggagtca cagatcaaaa ctattctgga tcctttaacc ttggtgcagg 241 gcaaccaaaa tgaagacaaa catctagtca cagataatcc aagtaaaatt aacccagaaa 301 ctgtagcaga gtggaagaaa aaactcagaa cagctaatga aatctatgaa aaagtgaaag 361 atgatgtgga taagctaaag gaggcaaata aaaaattgaa attggaaaat ggtggtctgg 421 tgagggagaa tttacgactg aaggctgaag ttgataacag atcacctcaa aagtttggaa 481 ggtttgcagt tgctgctctt cagtccaaag tagaacagta tgagcgtgaa accaatcgcc 541 tcaagaaagc cctggaacga agtgataagt atatagagga actagaatct caagttgcac 601 agctaaaaaa ttcaagtgaa gagaaagaag ctatgaattc catttgccag acagcacttt 661 ctgcagatgg caaagggagc aaaggcagtg aggaggatgt ggtgtcaaag aatcaaggcg 721 atagtgccag aaagcagcct ggctcatcca cctccagttc ttctcaccta gcgaagcctt 781 ccagcagcag actgtgtgac accagttctg caaggcagga aagtaccagc aaagcagacc 841 ttaactgttc taagaacaaa gacctatatc aagaacaggt agaagtaatg ttagatgtga 901 cagatacaag tatggatact tatttggaaa gagaatgggg gaataaacca agtgactgtg 961 taccctacaa agatgaagaa ctttatgatc ttccagctcc ttgtactcct ttgtccctta 1021 gttgccttca gctcagtact ccagaaaata gagagagctc tgtggtccaa gcaggaggtt 1081 ccaaaaagca ctcaaaccat ctcagaaaat tggtgtttga tgatttttgt gattcttcaa 1141 atgtttctaa taaagattct tcagaagatg atataagtag aagtgaaaat gaaaagaaat 1201 cagaatgttt ttcttcccca aagacaggat tttgggactg ttgttccaca agctatgccc 1261 aaaacttaga ttttgaaagt tcagagggga acacgatagc aaattctgtt GGAGAAATAT 1321 CTTCAAAATT GAGTGAGAAA TCAGGCTTAT GTTTATCCAA AAGGTTGAAT TCTATTCGCT 1381 CTTTTGAAAT GAACCGGACA AGAACATCCA GTGAAGCATC GATGGATGCT GCTTACCTTG 1441 ACAAAATCTC TGAGTTGGAT TCAATGATGT CAGAGTCAGA CAACAGCAAG AGCCCTTGTA 1501 ATAACGGTTT TAAGTCACTG GATTTGGATG GGTTATCAAA GTCATCTCAA GGCAGTGAAT 1561 TTCTTGAGGA ACCTGATAAG TTGGAAGAAA AAACTGAGCT AAACCTTTCC AAAGGTTCTC 1621 TAACTAATGA TCAGTTAGAA AATGGAAGTG AATGGAAACC CACTTCTTTT TTTTCTCCTC 1681 TCTCCATCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1741 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1801 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGGCGC GGGCATTGTA TCTGGCCCAC 1861 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt