Transcript: Human NM_024546.4

Homo sapiens ORC ubiquitin ligase 1 (OBI1), mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
OBI1 (79596)
Length:
3507
CDS:
36..2216

Additional Resources:

NCBI RefSeq record:
NM_024546.4
NBCI Gene record:
OBI1 (79596)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024546.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000285989 AGCAGTTCACATCGACTATTT pLKO_005 2004 CDS 100% 13.200 18.480 N OBI1 n/a
2 TRCN0000277660 ATGTATCTCGGTTGGATATAC pLKO_005 2524 3UTR 100% 13.200 18.480 N OBI1 n/a
3 TRCN0000137082 CCTGGAACGAAGTGATAAGTA pLKO.1 752 CDS 100% 5.625 7.875 N OBI1 n/a
4 TRCN0000277659 TCCAAAGGTTCTCTAACTAAT pLKO_005 1809 CDS 100% 13.200 10.560 N OBI1 n/a
5 TRCN0000136897 CACTGGATTTGGATGGGTTAT pLKO.1 1717 CDS 100% 10.800 8.640 N OBI1 n/a
6 TRCN0000285990 CACTGGATTTGGATGGGTTAT pLKO_005 1717 CDS 100% 10.800 8.640 N OBI1 n/a
7 TRCN0000134397 GTGAGAAATCAGGCTTATGTT pLKO.1 1534 CDS 100% 5.625 4.500 N OBI1 n/a
8 TRCN0000134953 GATAAGCTAAAGGAGGCAAAT pLKO.1 570 CDS 100% 10.800 7.560 N OBI1 n/a
9 TRCN0000137111 CCAGTTCTTGTCCAGTAACTA pLKO.1 1924 CDS 100% 5.625 3.938 N OBI1 n/a
10 TRCN0000277597 CCAGTTCTTGTCCAGTAACTA pLKO_005 1924 CDS 100% 5.625 3.938 N OBI1 n/a
11 TRCN0000133667 GCTTCATTGTTCCAGATGTAA pLKO.1 3047 3UTR 100% 5.625 3.938 N OBI1 n/a
12 TRCN0000125457 CAGTCCAAAGTAGAACAGTAT pLKO.1 702 CDS 100% 4.950 2.970 N Rnf219 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024546.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12571 pDONR223 100% 74.4% 74.1% None 1_231del;1832_1833insT;1854_2178del n/a
2 ccsbBroad304_12571 pLX_304 0% 74.4% 74.1% V5 1_231del;1832_1833insT;1854_2178del n/a
3 TRCN0000473310 CGGCGCGGGCATTGTATCTGGCCC pLX_317 23.6% 74.4% 74.1% V5 1_231del;1832_1833insT;1854_2178del n/a
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