Construct: ORF TRCN0000473338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008740.1_s317c1
- Derived from:
- ccsbBroadEn_03519
- DNA Barcode:
- TTTTCCTGATGTTTTGTGGGTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC186 (55088)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473338
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55088 | CCDC186 | coiled-coil domain containi... | NM_001321829.1 | 100% | 100% | |
| 2 | human | 55088 | CCDC186 | coiled-coil domain containi... | NM_018017.4 | 100% | 100% | |
| 3 | human | 55088 | CCDC186 | coiled-coil domain containi... | XM_011539915.3 | 72.4% | 72.4% | 0_1ins741 |
| 4 | human | 55088 | CCDC186 | coiled-coil domain containi... | XM_011539916.3 | 64.2% | 60.3% | (many diffs) |
| 5 | human | 55088 | CCDC186 | coiled-coil domain containi... | NR_135815.1 | 33.2% | (many diffs) | |
| 6 | human | 55088 | CCDC186 | coiled-coil domain containi... | NM_153249.1 | 23.4% | 23.4% | 633_634ins2061 |
| 7 | mouse | 213993 | Ccdc186 | coiled-coil domain containi... | XM_006526852.1 | 51% | 54% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2763
- ORF length:
- 2694
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcagagaca gaccacatag cctctacttc ctctgataaa aatgttggga 121 aaacacctga attaaaggaa gactcatgca acttgttttc tggcaatgaa agcagcaaat 181 tagaaaatga gtccaaacta ttgtcattaa acactgataa aactttatgt caacctaatg 241 agcataataa tcgaattgaa gcccaggaaa attatattcc agatcatggt ggaggtgagg 301 attcttgtgc caaaacagac acaggctcag aaaattctga acaaatagct aattttccta 361 gtggaaattt tgctaaacat atttcaaaaa caaatgaaac agaacagaaa gtaacacaaa 421 tattggtgga attaaggtca tctacatttc cagaatcagc taatgaaaag acttattcag 481 aaagccccta tgatacagac tgcaccaaga aatttatttc aaaaataaag agcgtttcag 541 catcagagga tttgttggaa gaaatagaat ctgagctctt atctacggag tttgcagaac 601 atcgagtacc aaatggaatg aataagggag aacatgcatt agttctgttt gaaaagtgtg 661 tgcaagataa atatttgcag caggaacata tcataaaaaa gttaattaaa gaaaataaga 721 agcatcagga gctcttcgta gacatttgtt cagaaaaaga caatttaaga gaagaactaa 781 agaaaagaac agaaactgag aagcagcata tgaacacaat taaacagtta gaatcaagaa 841 tagaagaact taataaagaa gttaaagctt ccagagatca actaatagct caagacgtta 901 cagctaaaaa tgcagttcag cagttacaca aagagatggc ccaacggatg gaacaggcca 961 acaagaaatg tgaagaggca cgccaagaaa aagaagcaat ggtaatgaaa tatgtaagag 1021 gtgagaagga atctttagat cttcgaaagg aaaaagagac acttgagaaa aaacttagag 1081 atgcaaataa ggaacttgag aaaaacacta acaaaattaa gcagctttct caggagaaag 1141 gacggttgca ccagctgtat gaaactaagg aaggcgaaac gactagactc atcagagaaa 1201 tagacaaatt aaaggaagac attaactctc acgtcatcaa agtaaagtgg gcacaaaaca 1261 aattaaaagc tgaaatggat tcacacaagg aaaccaaaga taaactcaaa gaaacaacaa 1321 caaaattaac acaagcaaag gaagaagcag atcagatacg aaaaaactgt caggatatga 1381 taaaaacata tcaggagtca gaagaaatta aatcaaatga gcttgatgca aagcttagag 1441 tcacaaaagg agaacttgaa aaacaaatgc aagaaaaatc tgaccagcta gagatgcatc 1501 atgccaaaat aaaggaacta gaagatctga agagaacatt taaggagggt atggatgagt 1561 taagaacact gagaacaaag gtgaaatgtc tagaagatga acgattaaga acagaagatg 1621 aattatcaaa atataaggaa attattaatc gccaaaaagc tgaaattcag aatttattgg 1681 acaaggtgaa aactgcagat cagctacagg agcagcttca aagaggtaag caagaaattg 1741 aaaatttgaa agaagaagtg gaaagtctta attctttgat taatgaccta caaaaagaca 1801 tcgaaggcag taggaaaaga gaatctgagc tgctgctgtt tacagaaagg ctcactagta 1861 agaatgcaca gcttcagtct gaatccaatt ctttgcagtc acaatttgat aaagtttcct 1921 gtagtgaaag tcagttacaa agccagtgtg aacaaatgaa acagacaaat attaatttgg 1981 aaagtaggtt gttgaaagag gaagaactgc gaaaagagga agtccaaact ctgcaagctg 2041 aactcgcttg tagacaaaca gaagttaaag cattgagtac ccaggtagaa gaattaaaag 2101 atgagttagt aactcagaga cgtaaacatg cctctagtat caaggatctc accaaacaac 2161 ttcagcaagc acgaagaaaa ttagatcagg ttgagagtgg aagctatgac aaagaagtca 2221 gcagcatggg aagtcgttct agttcatcag ggtccctgaa tgctcGAAGC AGTGCAGAAG 2281 ATCGATCTCC AGAAAATACT GGGTCCTCAG TAGCTGTGGA TAACTTTCCA CAAGTAGATA 2341 AGGCCATGTT GATTGAGAGA ATAGTTAGGC TGCAAAAAGC ACATGCCCGG AAAAATGAAA 2401 AGATAGAATT TATGGAGGAC CACATCAAAC AACTGGTGGA AGAAATTAGG AAAAAAACAA 2461 AAATAATTCA AAGTTATATT TTACGAGAAG AATCAGGCAC ACTTTCTTCA GAGGCATCTG 2521 ATTTTAACAA AGTTCATTTA AGTAGACGGG GTGGCATCAT GGCATCTTTA TATACATCCC 2581 ATCCAGCTGA CAATGGATTA ACATTGGAGC TCTCTTTGGA AATCAACCGA AAATTACAGG 2641 CTGTTTTGGA GGATACGTTA CTAAAAAATA TTACTTTGAA GGAAAATCTA CAAACACTTG 2701 GAACAGAAAT AGAACGTCTT ATTAAACACC AGCATGAACT AGAACAGAGG ACAAAGAAAA 2761 CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2821 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2881 GGCTTTATAT ATCTTGTGGA AAGGACGATT TTCCTGATGT TTTGTGGGTG TGACGCGTTA 2941 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt