Construct: ORF TRCN0000473372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000129.3_s317c1
- Derived from:
- ccsbBroadEn_12016
- DNA Barcode:
- CTCCGCCTCCTCGTCGTGTAGAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK26 (51765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_001042452.2 | 38.7% | 32.4% | 335_785del;863_1062del |
| 2 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_016542.4 | 30.9% | 27.6% | (many diffs) |
| 3 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_001313744.1 | 29.3% | 28.3% | (many diffs) |
| 4 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_133729.2 | 28.5% | 27.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 501
- ORF length:
- 411
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCCACTC GCCGGTGGCT GTCCAAGTGC 121 CTGGGATGCA GAATAACATA GCTGATCCAG AAGAACTGTT CACAAAATTA GAGCGCATTG 181 GGAAAGGCTC ATTTGGGGAA GTTTTCAAAG GAATTGATAA CCGTACCCAG CAAGTCGTTG 241 CTATTAAAAT CATAGACCTT GAGGAAGCCG AAGATGAAAT AGAAGACATT CAGCAAGAAA 301 TAACTGTCTT GAGTCAATGT GACAGCTCAT ATGTAACAAA ATACTATGGG TCATATTTAA 361 AGGGGTCTAA ATTATGGATA ATAATGGAAT ACCTGGGCGG TGGTTCAGCA CTGGATCTTC 421 TTCGAGCTTT ACCACCGTAC GAAAGAAGCC TGATCCAAAG AAAGTACAGA ATGGGGCAGA 481 GCAAGATCTT GTGCAAACCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCC GCCTCCTCGT 661 CGTGTAGAGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt