Construct: ORF TRCN0000473383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013370.1_s317c1
- Derived from:
- ccsbBroadEn_13431
- DNA Barcode:
- AAGTCGTGTGCAGTGTTGCCGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VSTM2A (222008)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222008 | VSTM2A | V-set and transmembrane dom... | NM_001317843.2 | 99.1% | 99.1% | (many diffs) |
| 2 | human | 222008 | VSTM2A | V-set and transmembrane dom... | NM_182546.3 | 91.4% | 87.3% | (many diffs) |
| 3 | human | 222008 | VSTM2A | V-set and transmembrane dom... | XM_006715665.3 | 89.3% | 86.8% | (many diffs) |
| 4 | human | 222008 | VSTM2A | V-set and transmembrane dom... | XM_006715666.3 | 88.1% | 86.4% | (many diffs) |
| 5 | human | 222008 | VSTM2A | V-set and transmembrane dom... | NM_001301009.1 | 86.9% | 80.9% | (many diffs) |
| 6 | human | 222008 | VSTM2A | V-set and transmembrane dom... | XM_017011841.1 | 66.4% | 62.4% | (many diffs) |
| 7 | human | 222008 | VSTM2A | V-set and transmembrane dom... | NR_133927.1 | 19.2% | (many diffs) | |
| 8 | mouse | 211739 | Vstm2a | V-set and transmembrane dom... | NM_145967.2 | 80.5% | 79.6% | (many diffs) |
| 9 | mouse | 211739 | Vstm2a | V-set and transmembrane dom... | XM_006514606.2 | 77.2% | 78.6% | (many diffs) |
| 10 | mouse | 211739 | Vstm2a | V-set and transmembrane dom... | NM_001290539.1 | 76.8% | 73% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 795
- ORF length:
- 729
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gatctttttg gtgtatgttg gatttgtttt cttttccgtt ttatatgtac 121 aacaagggct ttcttctcaa gcaaaattta ccgagtttcc gcggaacgtg acggcgaccg 181 aggggcagaa tgtggagatg tcctgcgcct tccagagcgg ctccgcctcg gtgtatctgg 241 agatccaatg gtggttcctg cgggggccgg aggacctgga tcccggggcc gagggggccg 301 gcgcgcaggt gaagctcttg cccGACAGAG ACCCGGACAG CGACGGGACC AAGATCAGCA 361 CAGTGAAAGT CCAAGGCAAT GACATCTCCC ACAAGCTTCA GATTTCCAAA GTGAGGAAAA 421 AGGATGAAGG CTTATATGAG TGCAGGGTGA CTGATGCCAA CTACGGGGAG CTTCAGGAAC 481 ACAAGGCCCA AGCCTATCTG AAAGTCAATG CAAACAGCCA TGCCCGCAGA ATGCAGGCCT 541 TCGAAGCCTC GCCCATGTGG CTGCAGGATA TGAAGCCCCG CAAGAACGTC TCCGCAGCCA 601 TCCCCAGCAG CATCCATGGC TCTGCCAACC AACGAACGCA CTCCACCTCC AGCCCTCAAG 661 TGGTAGCCAA AATCCCCAAA CAAAGTCCAC AATCAGGTAT GGAAACCCAT TTCGAGCCTT 721 TTATTTTACC ACTCACAAAC GCTCCACAGA AAGGTCAGTC GTATAGAGTA GACAGATTTA 781 TGAATGGTGA TTTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 841 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 901 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAGTCGTGTG CAGTGTTGCC 961 GGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt