Construct: ORF TRCN0000473425

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006527.1_s317c1
Derived from:
ccsbBroadEn_15543
DNA Barcode:
ACAACTGTGCAGAGGTGGGGGGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMA6 (5687)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473425
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5687 PSMA6 proteasome 20S subunit alpha 6 NM_002791.3 99.8% 100% 576A>G
2 human 5687 PSMA6 proteasome 20S subunit alpha 6 NM_001282234.1 91.3% 91% (many diffs)
3 human 5687 PSMA6 proteasome 20S subunit alpha 6 NM_001282232.1 67.7% 67.8% 0_1ins237;339A>G
4 human 5687 PSMA6 proteasome 20S subunit alpha 6 NM_001282233.1 67.7% 67.8% 0_1ins237;339A>G
5 human 5687 PSMA6 proteasome 20S subunit alpha 6 XM_017021468.1 67.7% 67.8% 0_1ins237;339A>G
6 human 5687 PSMA6 proteasome 20S subunit alpha 6 NR_104110.1 51.2% 1_160del;569_570ins179;720_912del
7 mouse 26443 Psma6 proteasome (prosome, macrop... NM_011968.3 93.4% 99.5% (many diffs)
8 mouse 26443 Psma6 proteasome (prosome, macrop... NM_001310583.1 85.9% 89% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
804
ORF length:
738
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ccgtggttcc agcgccggtt ttgaccgcca cattaccatt ttttcacccg
121agggtcggct ctaccaagta gaatatgctt ttaaggctat taaccagggt ggccttacat
181cagtagctgt cagagggaaa gactgtgcag taattgtcac acagaagaaa gtacctgaca
241aattattgga ttccagcaca gtgactcact tattcaagat aactgaaaac attggttgtg
301tgatgaccgg aatgacagct gacagcagat cccaggtaca gagggcacgc tatgaggcag
361ctaactggaa atacaagtat ggctatgaga ttcctgtgga catgctgtgt aaaagaattg
421ccgatatttc tcaggtctac acacagaatg ctgaaatgag gcctcttggt tgttgtatga
481ttttaattgg tatagatgaa gagcaaggcc cTCAGGTATA TAAGTGTGAT CCTGCAGGTT
541ACTACTGTGG GTTTAAAGCC ACTGCAGCGG GAGTTAAACA AACTGAGTCA ACCAGCTTCC
601TTGAAAAAAA AGTGAAGAAG AAATTTGATT GGACATTTGA GCAGACAGTG GAAACTGCAA
661TTACATGCCT GTCTACTGTT CTATCAATTG ATTTCAAACC TTCAGAAATA GAAGTTGGAG
721TAGTGACAGT TGAAAATCCT AAATTCAGGA TTCTTACAGA AGCAGAGATT GATGCTCACC
781TTGTTGCTCT AGCAGAGAGA GACTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
841AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
901TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CAACTGTGCA
961GAGGTGGGGG GATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1021att

Download FASTA (ORF) (Full)