Construct: ORF TRCN0000473433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009459.1_s317c1
- Derived from:
- ccsbBroadEn_14304
- DNA Barcode:
- ATGACGGTTCTCGCTTTCACACCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC41A2 (84102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84102 | SLC41A2 | solute carrier family 41 me... | NM_001352169.1 | 85.4% | 85.1% | 1_249del;1712delT |
| 2 | human | 84102 | SLC41A2 | solute carrier family 41 me... | NM_001352170.2 | 85.4% | 85.1% | 1_249del;1712delT |
| 3 | human | 84102 | SLC41A2 | solute carrier family 41 me... | NM_001352171.2 | 85.4% | 85.1% | 1_249del;1712delT |
| 4 | human | 84102 | SLC41A2 | solute carrier family 41 me... | NM_001352172.2 | 85.4% | 85.1% | 1_249del;1712delT |
| 5 | human | 84102 | SLC41A2 | solute carrier family 41 me... | NM_032148.5 | 85.4% | 85.1% | 1_249del;1712delT |
| 6 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_005269179.1 | 85.4% | 85.1% | 1_249del;1712delT |
| 7 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_011538808.1 | 85.4% | 85.1% | 1_249del;1712delT |
| 8 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_011538810.2 | 85.4% | 85.1% | 1_249del;1712delT |
| 9 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_011538811.2 | 85.4% | 85.1% | 1_249del;1712delT |
| 10 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_017020013.1 | 85.4% | 85.1% | 1_249del;1712delT |
| 11 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_024449219.1 | 85.4% | 85.1% | 1_249del;1712delT |
| 12 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_017020014.1 | 76.5% | 75.6% | (many diffs) |
| 13 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_017020016.2 | 73.8% | 73.4% | 0_1ins384;1079delT |
| 14 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_017020017.1 | 72.9% | 72% | (many diffs) |
| 15 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_011538813.2 | 70.7% | 70.4% | 0_1ins429;1034delT |
| 16 | human | 84102 | SLC41A2 | solute carrier family 41 me... | XM_017020015.1 | 54.4% | 54.1% | (many diffs) |
| 17 | mouse | 338365 | Slc41a2 | solute carrier family 41, m... | NM_177388.3 | 72.4% | 78.3% | (many diffs) |
| 18 | mouse | 338365 | Slc41a2 | solute carrier family 41, m... | XM_006513811.3 | 72.4% | 78.3% | (many diffs) |
| 19 | mouse | 338365 | Slc41a2 | solute carrier family 41, m... | XM_011243498.2 | 72.4% | 78.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1536
- ORF length:
- 1470
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtatcacagt ttctcagagc agtcttttca tgccaataat gggcacgcat 121 catcaagctg cagccaaaag tatgatgact atgccaatta taattactgt gatggaaggg 181 agacttcaga aaccactgcc atgttacaag atgaagatat atctagtgat ggtgatgaag 241 atgctattgt agaagtgacc ccaaaattac caaaggaatc cagtggcatc atggcattgc 301 aaatacttgt gccctttttg ctagctggtt ttggaacagt ttcagctggc atggtactgg 361 atatagtaca gcactgggag gtgttcagaa aagttacaga agttttcatt ttagtccctg 421 cacttcttgg tctcaaaggg aacttggaaa tgacattggc atccagatta tccactgcag 481 taaatattgg gaagatggat tcacccattg aaaagtggaa cctaataatt ggcaacttgg 541 ctttaaagca ggttcaggca acagtagtgg gttttctagc agctgtggca gcaattatat 601 tgggctggat tccagaagga aaatattacc ttgatcattc catacttctg tgctctagca 661 gtgtggcaac tgccttcatt gcatctcttc tgcagggaat aataatggtt ggggttatcg 721 ttggttcaaa gaagactggt ataaatcctg ataatgttgc tacacccatt gctgctagtt 781 ttggcgacct tataactctt gccatattgg cttggataag tcagggctta tactcctgtc 841 ttgagaccta ttactacatt tctccattag ttggtgtatt tttcttggct ctaaccccta 901 tttggattat aatagctgcc aaacatccag ccacaagaac agttctccac tcaggctggg 961 agcctgtcat aacagctatg gttataagta gcattggggg ccttattctg gacacaactg 1021 tatcagaccc aaacttggtt gggattgttg tttacacgcc agttattaat ggtattggtg 1081 gtaatttggt ggccattcag gctagcagga tttctaccta cctccatttA CATAGCATTC 1141 CAGGAGAATT GCCTGATGAA CCCAAAGGTT GTTACTACCC ATTTAGAACT TTCTTTGGTC 1201 CAGGAGTAAA TAATAAGTCT GCTCAAGTTC TACTGCTTTT AGTGATTCCT GGACATTTAA 1261 TTTTCCTCTA CACTATTCAT TTGATGAAAA GTGGTCATAC TTCTTTAACT ATAATCTTCA 1321 TAGTAGTGTA TTTATTTGGC GCTGTGTTAC AGGTATTTAC CTTGCTGTGG ATTGCTGACT 1381 GGATGGTCCA TCACTTCTGG AGGAAAGGAA AGGACCCGGA TAGTTTCTCC ATCCCCTACC 1441 TAACAGCATT GGGTGATCTG CTCGGGACAG CTCTGTTAGC CTTAAGTTTT CATTTTCTTT 1501 GGCTTATTGG AGATCGAGAT GGAGATGTGG AGACTACCCA ACTTTCTTGT ACAAAGTGGT 1561 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 1621 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 1681 ATGACGGTTC TCGCTTTCAC ACCCACGCGT TAAGTCgaca atcaacctct ggattacaaa 1741 atttgtgaaa gatt