Construct: ORF TRCN0000473443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014352.1_s317c1
- Derived from:
- ccsbBroadEn_11771
- DNA Barcode:
- TTTCTGCTCTAGGACCTTCAATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SULT4A1 (25830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | NM_014351.4 | 89.7% | 87.6% | (many diffs) |
| 2 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XM_011530120.3 | 65.4% | 65.3% | 509delA;513_513delAins269 |
| 3 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XM_024452212.1 | 62.1% | 59.8% | (many diffs) |
| 4 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XR_937839.3 | 56.5% | (many diffs) | |
| 5 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XM_011530121.1 | 50.2% | 47.5% | (many diffs) |
| 6 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XR_001755207.1 | 31.3% | (many diffs) | |
| 7 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XR_937838.2 | 30.9% | 1_50del;831_2524del | |
| 8 | mouse | 29859 | Sult4a1 | sulfotransferase family 4A,... | XM_006521065.3 | 84.4% | 88.9% | (many diffs) |
| 9 | mouse | 29859 | Sult4a1 | sulfotransferase family 4A,... | NM_013873.3 | 82% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 846
- ORF length:
- 780
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagagcgag gccgagaccc ccagcacccc gggggagttc gagagcaagt 121 acttcgagtt ccatggcgtg cggctgccgc ccttctgccg cgggaagatg gaggagatcg 181 ccaacttccc ggtgcggccc agcgacgtgt ggatcgtcac ctaccccaag tccggcacca 241 gcttgctgca ggaggtggtc tacttggtga gccagggcgc tgaccccgat gagatcggct 301 tgatgaacat cgacgagcag ctcccggtcc tggagtaccc acagccgggc ctggacatca 361 tcaaggaact gacctctccc cgcctcatca agagccacct gccctaccgc tttctgccct 421 ctgacctcca caatggagac tccaaggtca tctatatggc tcgcaacccc aaggatctgg 481 tggtgtctta ttatcagttc caccgctctc TGCGGACCAT GAGCTACCGA GGCACCTTTC 541 AAGAATTCTG CCGGAGGTTT ATGAATGATA AGCTGGGCTA CGGCTCCTGG TTTGAGCACG 601 TGCAGGAGTT CTGGGAGCAC CGCATGGACT CGAACGTGCT TTTTCTCAAG TATGAAGACA 661 TGCATCGGGA CCTGGTGACG ATGGTGGAGC AGCTGGCCAG ATTCCTGGGG GTGTCCTGTG 721 ACAAGGCCCA GCTGGAAGCC CTGACGGAGC ACTGCCACCA GCTGGTGGAC CAGTGCTGCA 781 ACGCTGAGGC CCTGCCCGTG GGCCGGGCAC ATTGCGTCTT TGCTCGGAAG ATCTTCTTGA 841 GTTGGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTTCTGCTC TAGGACCTTC AATGAACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt