Construct: ORF TRCN0000473466
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017749.1_s317c1
- Derived from:
- ccsbBroadEn_04354
- DNA Barcode:
- TAACTGAGGCGCGGAGGGACCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NICN1 (84276)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473466
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84276 | NICN1 | nicolin 1 | NM_032316.3 | 100% | 100% | |
| 2 | mouse | 66257 | Nicn1 | nicolin 1 | NM_025449.3 | 86.5% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccgcgttttg gtgccttgcc atgtgaaagg ctccgtagcc ctccaggtgg 121 gcgacgtgcg gacctcccaa ggccggcctg gcgtgctggt catcgatgtc accttcccca 181 gcgtcgctcc cttcgagttg caggaaatca cgtttaagaa ttactacaca gcttttttga 241 gcatccgtgt ccgtcagtac accTCAGCAC ACACACCTGC CAAGTGGGTG ACCTGCCTGC 301 GGGACTACTG CCTAATGCCT GACCCACACA GTGAGGAGGG AGCCCAGGAG TATGTATCGC 361 TGTTCAAGCA TCAGATGCTG TGTGACATGG CTAGAATATC GGAGCTACGC CTGATTCTGC 421 GGCAGCCATC ACCACTGTGG CTGTCTTTCA CAGTGGAGGA GCTGCAGATC TATCAGCAGG 481 GACCAAAGAG CCCCTCCGTG ACCTTTCCCA AGTGGCTCTC CCACCCAGTG CCCTGTGAGC 541 AACCTGCACT CCTCCGTGAG GGTCTCCCAG ACCCCAGCAG GGTATCCTCC GAGGTGCAGC 601 AGATGTGGGC ACTGACAGAG ATGATCCGGG CCAGTCACAC CTCCGCAAGG ATCGGCCGCT 661 TTGATGTGGA TGGCTGTTAT GACCTGAACT TGCTCTCCTA CACTTGCCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TAACTGAGGC GCGGAGGGAC CGACACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt