Transcript: Human NM_032316.3

Homo sapiens nicolin 1 (NICN1), mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
NICN1 (84276)
Length:
3227
CDS:
86..727

Additional Resources:

NCBI RefSeq record:
NM_032316.3
NBCI Gene record:
NICN1 (84276)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_032316.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000122007 GTTGCAGGAAATCACGTTTAA pLKO.1 217 CDS 100% 13.200 10.560 N NICN1 n/a
2 TRCN0000141444 CCTGGCTGCAACTCAATTCTT pLKO.1 933 3UTR 100% 5.625 3.938 N NICN1 n/a
3 TRCN0000122257 CCAAGAGATATGCTCAGCATA pLKO.1 2095 3UTR 100% 4.950 3.465 N NICN1 n/a
4 TRCN0000143631 GAGTATGTATCGCTGTTCAAG pLKO.1 368 CDS 100% 4.950 3.465 N NICN1 n/a
5 TRCN0000122149 GCAACTCAATTCTTCCTTCTA pLKO.1 940 3UTR 100% 4.950 3.465 N NICN1 n/a
6 TRCN0000141724 GCTTTGATGTGGATGGCTGTT pLKO.1 678 CDS 100% 4.050 2.835 N NICN1 n/a
7 TRCN0000141384 CTGAACTTGCTCTCCTACACT pLKO.1 704 CDS 100% 3.000 2.100 N NICN1 n/a
8 TRCN0000144735 GAAGTGTATTTCCTGTGCATT pLKO.1 797 3UTR 100% 4.950 2.970 N NICN1 n/a
9 TRCN0000034867 CCTTGTGTCGTCCACTTAGTT pLKO.1 3171 3UTR 100% 5.625 2.813 Y AMT n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1429 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000122309 CCTGAGCATAAGAACTGGGAT pLKO.1 1382 3UTR 100% 2.640 1.584 N NICN1 n/a
12 TRCN0000162795 CTTCCAAAGTGCTGGGATTAT pLKO.1 2041 3UTR 100% 13.200 6.600 Y SLC48A1 n/a
13 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1430 3UTR 100% 13.200 6.600 Y LIAS n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_032316.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04354 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04354 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000473466 TAACTGAGGCGCGGAGGGACCGAC pLX_317 68.5% 100% 100% V5 n/a
Download CSV