Construct: ORF TRCN0000473471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011870.1_s317c1
- Derived from:
- ccsbBroadEn_01441
- DNA Barcode:
- GGCAACGGAACGTTATTAGGTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPLP0 (6175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6175 | RPLP0 | ribosomal protein lateral s... | NM_001002.4 | 100% | 100% | |
| 2 | human | 6175 | RPLP0 | ribosomal protein lateral s... | NM_053275.3 | 100% | 100% | |
| 3 | human | 113157 | RPLP0P2 | ribosomal protein lateral s... | NR_002775.2 | 25.4% | (many diffs) | |
| 4 | mouse | 11837 | Rplp0 | ribosomal protein, large, P0 | NM_007475.5 | 90.4% | 97.4% | (many diffs) |
| 5 | mouse | 544707 | Gm5779 | predicted gene 5779; riboso... | NR_033602.1 | 83.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cagggaagac agggcgacct ggaagtccaa ctacttcctt aagatcatcc 121 aactattgga tgattatccg aaatgtttca ttgtgggagc agacaatgtg ggctccaagc 181 agatgcagca gatccgcatg tcccttcgcg ggaaggctgt ggtgctgatg ggcaagaaca 241 ccatgatgcg caaggccatc cgagggcacc tggaaaacaa cccagctctg gagaaactgc 301 tgcctcatat ccgggggaat gtgggctttg tgttcaccaa ggaggacctc actgagatca 361 gggacatgtt gctggccaat aaggtgccag ctgctgcccg tgctggtgcc attgccccat 421 gtgaagtcac tgtgccagcc cagaacactg gtctcgggcc cgagaagacc tcctttttcc 481 aggctttagg tatcaccact aaaatctcca ggggcaccat tgaaatcctg agtgatgtgc 541 agctgatcaa gactggagac aaagtgggag ccagcgaagc cacgctgctg aacatgctca 601 acatctcccc cttctccttt gggctggTCA TCCAGCAGGT GTTCGACAAT GGCAGCATCT 661 ACAACCCTGA AGTGCTTGAT ATCACAGAGG AAACTCTGCA TTCTCGCTTC CTGGAGGGTG 721 TCCGCAATGT TGCCAGTGTC TGTCTGCAGA TTGGCTACCC AACTGTTGCA TCAGTACCCC 781 ATTCTATCAT CAACGGGTAC AAACGAGTCC TGGCCTTGTC TGTGGAGACG GATTACACCT 841 TCCCACTTGC TGAAAAGGTC AAGGCCTTCT TGGCTGATCC ATCTGCCTTT GTGGCTGCTG 901 CCCCTGTGGC TGCTGCCACC ACAGCTGCTC CTGCTGCTGC TGCAGCCCCA GCTAAGGTTG 961 AAGCCAAGGA AGAGTCGGAG GAGTCGGACG AGGATATGGG ATTTGGTCTC TTTGACTACC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GAGGCAACGG AACGTTATTA GGTACTACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt