Construct: ORF TRCN0000473474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000268.1_s317c1
- Derived from:
- ccsbBroadEn_14281
- DNA Barcode:
- TTGTATCTCTTCCGAGTCCTCCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP290 (80184)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538766.3 | 7.2% | 7.1% | (many diffs) |
| 2 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538765.3 | 6.9% | 6.8% | (many diffs) |
| 3 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538764.3 | 6.9% | 6.8% | (many diffs) |
| 4 | human | 80184 | CEP290 | centrosomal protein 290 | XM_017019983.2 | 6.5% | 6.5% | (many diffs) |
| 5 | human | 80184 | CEP290 | centrosomal protein 290 | NM_025114.4 | 6.5% | 6.5% | (many diffs) |
| 6 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538763.3 | 6.5% | 6.5% | (many diffs) |
| 7 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538762.3 | 6.4% | 6.4% | (many diffs) |
| 8 | human | 80184 | CEP290 | centrosomal protein 290 | XM_017019982.1 | 6.2% | 6.2% | (many diffs) |
| 9 | human | 80184 | CEP290 | centrosomal protein 290 | XM_017019981.2 | 6% | 5.9% | (many diffs) |
| 10 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538761.2 | 6% | 5.9% | (many diffs) |
| 11 | human | 80184 | CEP290 | centrosomal protein 290 | XM_017019980.2 | 5.9% | 5.9% | (many diffs) |
| 12 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538760.2 | 5.9% | 5.9% | (many diffs) |
| 13 | human | 80184 | CEP290 | centrosomal protein 290 | XR_001748870.2 | 5.9% | (many diffs) | |
| 14 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538759.2 | 5.9% | 5.8% | (many diffs) |
| 15 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538758.3 | 5.9% | 5.8% | (many diffs) |
| 16 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538756.3 | 5.8% | 5.8% | (many diffs) |
| 17 | human | 80184 | CEP290 | centrosomal protein 290 | XM_011538757.3 | 5.8% | 5.8% | (many diffs) |
| 18 | human | 80184 | CEP290 | centrosomal protein 290 | XR_001748869.1 | 5.8% | (many diffs) | |
| 19 | mouse | 216274 | Cep290 | centrosomal protein 290 | XM_006513528.2 | 5.8% | 6.1% | (many diffs) |
| 20 | mouse | 216274 | Cep290 | centrosomal protein 290 | NM_146009.2 | 5.8% | 6% | (many diffs) |
| 21 | mouse | 216274 | Cep290 | centrosomal protein 290 | XM_006513527.2 | 5.8% | 6% | (many diffs) |
| 22 | mouse | 216274 | Cep290 | centrosomal protein 290 | XM_006513526.2 | 5.2% | 5.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cattttcaag agtgcagctc tccaaaaagt tgtagataat agtgtttctt 121 tgtctgaact agaactggct aataaacagt acaatgaact gactgctaag tacagggaca 181 tcttgcaaaa agataatatg cttGTTCAAA GAACAAGTAA CTTGGAACAC CTGGAGTGTG 241 AAAACATCTC CTTAAAAGAA CAAGTGGAGT CTATAAATAA AGAACTGGAG ATTACCAAGG 301 AAAAACTTCA CACTATTGAA CAAGCCTGGG AACAGGAAAC TAAATTAGGT AATGAATCTA 361 GCATGGATAA GGCAAAGAAA TCAATAACCA ACAGTGACAT TGTTTCCATT TCAAAAAAAA 421 TAACTATGCT GGAAATGAAG GAATTAAATG AAAGGCAGCG GGCTGAACAT TGTCAAAAAA 481 TGTATGAACA CTTACGGACT TCGTTAAAGC AAATGGAGGA ACGTAATTTT GAATTGGAAA 541 CCAAATTTGC TGAGGTTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGTATC TCTTCCGAGT 721 CCTCCGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt