Construct: ORF TRCN0000473501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013936.1_s317c1
- Derived from:
- ccsbBroadEn_09309
- DNA Barcode:
- ATCGACCGTAATGGTCATCTACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CREB3L1 (90993)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473501
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90993 | CREB3L1 | cAMP responsive element bin... | NM_052854.4 | 99.9% | 99.8% | 1049T>C |
| 2 | human | 90993 | CREB3L1 | cAMP responsive element bin... | XM_006718380.3 | 68% | 64.9% | (many diffs) |
| 3 | mouse | 26427 | Creb3l1 | cAMP responsive element bin... | NM_011957.2 | 88.8% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgccgtcttg gaacccttcc cggccgacag gctgttcccc ggatccagct 121 tcctggactt gggggatctg aacgagtcgg acttcctcaa caatgcgcac tttcctgagc 181 acctggacca ctttacggag aacatggagg acttctccaa tgacctgttc agcagcttct 241 ttgatgaccc tgtgctggat gagaagagcc ctctattgga catggaactg gactccccta 301 cgccaggcat ccaggcggag cacagctact ccctgagcgg cgactcagcg ccccagagcc 361 cccttgtgcc catcaagatg gaggacacca cccaagatgc agagcatgga gcatgggcgc 421 tgggacacaa actgtgctcc atcatggtga agcaggagca gagcccggag ctgcccgtgg 481 accctctggc tgccccctcg gccatggctg ccgcggccgc catggccacc accccgctgc 541 tgggcctcag ccccttgtcc aggctgccca tcccccacca ggccccggga gagatgactc 601 agctgccagt gatcaaagca gagcctctgg aggtgaacca gttcctcaaa gtgacaccgg 661 aggacctggt gcagatgcct ccgacgcccc ccagcagcca tggcagtgac agcgacggct 721 cccagagtcc ccgctctctg cccccctcca gccctgtcag gcccatggcg cgctcctcca 781 cggccatctc cacctcccca ctcctcactg cccctcacaa attacagggg acatcagggc 841 cactgctcct gacagaggag gagaagcgga ccctgattgc tgagggctac cccatcccca 901 caaaactccc cctcaccaaa gccgaggaga aggccttgaa gagagtccgg aggaaaatca 961 agaacaagat ctcagcccag gagagccgtc gtaagaagaa ggagtatgtg gagtgtctag 1021 aaaagaaggt ggagacattt acatctgaga acaatgaact gtggaagaag gtggagaccc 1081 tggagaatgc caacaggacc ctgctccagc agccgcagaa actccagact ctggtcacca 1141 acaagatctc cagaccttac aagatggccg ccacccagac tgGGACCTGC CTCATGGTGG 1201 CAGCCTTGTG CTTTGTTCTG GTGCTGGGCT CCCTCGTGCC CTGCCTTCCC GAGTTCTCCT 1261 CCGGCTCCCA GACTGTGAAG GAAGACCCCC TGGCCGCAGA CGGCGTCTAC ACGGCCAGCC 1321 AGATGCCCTC CCGAAGCCTC CTATTCTACG ATGACGGGGC AGGCTTATGG GAAGATGGCC 1381 GCAGCACCCT GCTGCCCATG GAGCCCCCAG ATGGCTGGGA AATCAACCCC GGGGGGCCGG 1441 CAGAGCAGCG GCCCCGGGAC CACCTGCAGC ATGATCACCT GGACAGCACC CACGAGACCA 1501 CCAAGTACCT GAGTGAGGCC TGGCCTAAAG ACGGTGGAAA CGGCACCAGC CCCGACTTCT 1561 CCCACTCCAA GGAGTGGTTC CACGACAGGG ATCTGGGCCC CAACACCACC ATCAAACTCT 1621 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAAT CGACCGTAAT GGTCATCTAC CCACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt